TPR2_ARATH
ID TPR2_ARATH Reviewed; 1131 AA.
AC Q9LRZ0;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 10-AUG-2010, sequence version 2.
DT 25-MAY-2022, entry version 147.
DE RecName: Full=Topless-related protein 2;
GN Name=TPR2; OrderedLocusNames=At3g16830; ORFNames=K20I9.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16763149; DOI=10.1126/science.1123841;
RA Long J.A., Ohno C., Smith Z.R., Meyerowitz E.M.;
RT "TOPLESS regulates apical embryonic fate in Arabidopsis.";
RL Science 312:1520-1523(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-214, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [6]
RP INTERACTION WITH AFPH2.
RX PubMed=20360743; DOI=10.1038/nature08854;
RA Pauwels L., Barbero G.F., Geerinck J., Tilleman S., Grunewald W.,
RA Perez A.C., Chico J.M., Bossche R.V., Sewell J., Gil E., Garcia-Casado G.,
RA Witters E., Inze D., Long J.A., De Jaeger G., Solano R., Goossens A.;
RT "NINJA connects the co-repressor TOPLESS to jasmonate signalling.";
RL Nature 464:788-791(2010).
RN [7]
RP INTERACTION WITH SPEAR3/TIE1.
RX PubMed=23444332; DOI=10.1105/tpc.113.109223;
RA Tao Q., Guo D., Wei B., Zhang F., Pang C., Jiang H., Zhang J., Wei T.,
RA Gu H., Qu L.J., Qin G.;
RT "The TIE1 transcriptional repressor links TCP transcription factors with
RT TOPLESS/TOPLESS-RELATED corepressors and modulates leaf development in
RT Arabidopsis.";
RL Plant Cell 25:421-437(2013).
RN [8]
RP INTERACTION WITH SPL.
RX PubMed=25527103; DOI=10.1016/j.jgg.2014.08.009;
RA Chen G.H., Sun J.Y., Liu M., Liu J., Yang W.C.;
RT "SPOROCYTELESS is a novel embryophyte-specific transcription repressor that
RT interacts with TPL and TCP proteins in Arabidopsis.";
RL J. Genet. Genomics 41:617-625(2014).
RN [9]
RP INTERACTION WITH SPL.
RX PubMed=25378179; DOI=10.1038/cr.2014.145;
RA Wei B., Zhang J., Pang C., Yu H., Guo D., Jiang H., Ding M., Chen Z.,
RA Tao Q., Gu H., Qu L.J., Qin G.;
RT "The molecular mechanism of sporocyteless/nozzle in controlling Arabidopsis
RT ovule development.";
RL Cell Res. 25:121-134(2015).
RN [10]
RP INTERACTION WITH SMXL6; SMXL7 AND SMXL8.
RX PubMed=26546446; DOI=10.1105/tpc.15.00605;
RA Wang L., Wang B., Jiang L., Liu X., Li X., Lu Z., Meng X., Wang Y.,
RA Smith S.M., Li J.;
RT "Strigolactone signaling in Arabidopsis regulates shoot development by
RT targeting D53-like SMXL repressor proteins for ubiquitination and
RT degradation.";
RL Plant Cell 27:3128-3142(2015).
RN [11]
RP SUBUNIT, AND DOMAIN.
RX PubMed=26601214; DOI=10.1126/sciadv.1500107;
RA Ke J., Ma H., Gu X., Thelen A., Brunzelle J.S., Li J., Xu H.E., Melcher K.;
RT "Structural basis for recognition of diverse transcriptional repressors by
RT the TOPLESS family of corepressors.";
RL Sci. Adv. 1:E1500107-E1500107(2015).
CC -!- FUNCTION: Transcriptional corepressor. Negative regulator of jasmonate
CC responses (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Tetramer (PubMed:26601214). Interacts with NINJA/AFPH2
CC (PubMed:20360743). Interacts with SMXL6, SMXL7 and SMXL8
CC (PubMed:26546446). Interacts with SPL (via EAR motif) (PubMed:25527103,
CC PubMed:25378179). Interacts with SPEAR3/TIE1 (PubMed:23444332).
CC {ECO:0000269|PubMed:20360743, ECO:0000269|PubMed:23444332,
CC ECO:0000269|PubMed:25378179, ECO:0000269|PubMed:25527103,
CC ECO:0000269|PubMed:26546446, ECO:0000269|PubMed:26601214}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The N-terminal TOPLESS domain (TPD) (1-209) binds directly to a
CC 12-amino acid LxLxL EAR motif peptide. {ECO:0000269|PubMed:26601214}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AY074277; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB028608; BAA95777.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75871.1; -; Genomic_DNA.
DR EMBL; AY074277; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_188306.2; NM_112557.4.
DR AlphaFoldDB; Q9LRZ0; -.
DR BioGRID; 6270; 51.
DR DIP; DIP-58586N; -.
DR IntAct; Q9LRZ0; 4.
DR MINT; Q9LRZ0; -.
DR STRING; 3702.AT3G16830.1; -.
DR iPTMnet; Q9LRZ0; -.
DR PaxDb; Q9LRZ0; -.
DR PRIDE; Q9LRZ0; -.
DR ProteomicsDB; 228410; -.
DR EnsemblPlants; AT3G16830.1; AT3G16830.1; AT3G16830.
DR GeneID; 820936; -.
DR Gramene; AT3G16830.1; AT3G16830.1; AT3G16830.
DR KEGG; ath:AT3G16830; -.
DR Araport; AT3G16830; -.
DR TAIR; locus:2086770; AT3G16830.
DR eggNOG; KOG0266; Eukaryota.
DR HOGENOM; CLU_003103_1_0_1; -.
DR InParanoid; Q9LRZ0; -.
DR OMA; DENRYSM; -.
DR OrthoDB; 94792at2759; -.
DR PhylomeDB; Q9LRZ0; -.
DR PRO; PR:Q9LRZ0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LRZ0; baseline and differential.
DR Genevisible; Q9LRZ0; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0010072; P:primary shoot apical meristem specification; IGI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 4.
DR InterPro; IPR006595; CTLH_C.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR027728; Topless_fam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR44083; PTHR44083; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00668; CTLH; 1.
DR SMART; SM00667; LisH; 1.
DR SMART; SM00320; WD40; 11.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
DR PROSITE; PS50897; CTLH; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Jasmonic acid signaling pathway; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; WD repeat.
FT CHAIN 1..1131
FT /note="Topless-related protein 2"
FT /id="PRO_0000394733"
FT DOMAIN 4..36
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT DOMAIN 34..92
FT /note="CTLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
FT REPEAT 345..385
FT /note="WD 1"
FT REPEAT 407..446
FT /note="WD 2"
FT REPEAT 451..493
FT /note="WD 3"
FT REPEAT 495..535
FT /note="WD 4"
FT REPEAT 585..624
FT /note="WD 5"
FT REPEAT 629..668
FT /note="WD 6"
FT REPEAT 763..802
FT /note="WD 7"
FT REPEAT 829..867
FT /note="WD 8"
FT REPEAT 870..910
FT /note="WD 9"
FT REPEAT 913..952
FT /note="WD 10"
FT REPEAT 959..999
FT /note="WD 11"
FT REPEAT 1005..1044
FT /note="WD 12"
FT REGION 1099..1131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1110..1131
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 214
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19376835"
SQ SEQUENCE 1131 AA; 124760 MW; 7AB804673A5359F4 CRC64;
MSSLSRELVF LILQFLDEEK FKESVHKLEQ ESGFFFNIKY FEEKALAGEW DEVEKYLSGF
TKVDDNRYSM KIFFEIRKQK YLEALDRNDR AKAVEILAKD LKVFATFNEE LYKEITQLLT
LENFRENEQL SKYGDTKSAR SIMYTELKKL IEANPLFREK LAFPSFKASR LRTLINQSLN
WQHQLCKNPR PNPDIKTLFL DHSCSPSNGA RALTPVNLPV AAVARPSNFV PLGVHGGPFQ
SNPAPAPNAN ALAGWMANPN PSSSVPSGVV AASPFPMQPS QVNELKHPRA PSNSLGLMDY
QSADHEQLMK RLRSAQTSNE VTYPAHSHPP ASLDDLPRNV VSTIRQGSVV ISMDFHPSHH
TLLAVGCSSG EVTLWEVGSR EKVVTEPFKI WNMAACSVIF QGSIVKEPSI SVTRVAWSPD
GNLLGVSFTK HLIHVYAYQG SDLRQHLEID AHVGCVNDLA FAHPNKQMCV VTCGDDKLIK
VWDLSGKKLF TFEGHEAPVY SICPHQKENI QFIFSTALDG KIKAWLYDNV GSRVDYDAPG
QWCTTMLYSA DGSRLFSCGT SKEGDSFLVE WNESEGALKR TYLGFRKKSA GVVQFDTTRN
RFLAVGEDNQ IKFWNMDNTN LLTVVEAEGG LPNLPRLRFN KDGNLLAVTT ADNGFKILAN
TDGLRTLRAF EARSFEASKA SIDMKVSTSA MASSISPAIG KIEHMDAGSP ARPTPIPNGI
EAMSRTMEKP RNLDSVDKSK PLELTEIVDP TQCRQVTMPD SKDSVSKVAR LLYTNSGVGV
LALGSNGVQR LWKWIRNEQN PTGKATASVT PQHWQPNSGL LMANDVPENP EGSVPCIALS
KNDSYVMSAC GGKVSLFNMM TFKVMTTFMP PPPASTFLAF HPQDNNIIAI GMEDSSIHIY
NVRVDEVKTK LKGHQKHITG LAFSTALNIL VSSGADAQLF FWTADSWEKK KSSAIQLPPG
KAPVGDTRVQ FHNDQIQLLV SHETQLAIYD ASKMECIHKW VPQEALSSPI TSASYSCNSQ
LVYASFADGN IAVFDAESLR LRCRIAPSAY MPQPTPNSAP IFPQVITAHP QEPNQLAVGL
SDGSVKVIEP SELSRRWGVG VAAGSDKAGT ENGRPSSSSA ANNSSSDQIQ R
