TPR2_ORYSJ
ID TPR2_ORYSJ Reviewed; 1133 AA.
AC Q0J7U6; Q7EYE2;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Protein TOPLESS-RELATED PROTEIN 2 {ECO:0000303|PubMed:24336200};
DE Short=Protein TPR2 {ECO:0000303|PubMed:24336200};
DE AltName: Full=Protein ABERRANT SPIKELET AND PANICLE 1 {ECO:0000303|PubMed:22136599};
DE Short=Protein ASP1 {ECO:0000303|PubMed:22136599};
DE AltName: Full=Protein LISSENCEPHALY TYPE-1-LIKE 1 {ECO:0000303|PubMed:22020753};
DE Short=OsLIS-L1 {ECO:0000303|PubMed:22020753};
DE Short=Protein LIS-L1 {ECO:0000303|PubMed:22020753};
DE AltName: Full=Protein TPL {ECO:0000303|PubMed:26601214};
DE Short=OsTPL {ECO:0000303|PubMed:26601214};
DE AltName: Full=Ramosa1 enhancer locus 2 {ECO:0000303|Ref.7};
DE Short=OsREL2 {ECO:0000303|Ref.7};
DE Short=Protein REL2 {ECO:0000303|Ref.7};
DE AltName: Full=Topless-like protein {ECO:0000303|PubMed:26601214};
GN Name=TPR2 {ECO:0000303|PubMed:24336200};
GN Synonyms=ASP1 {ECO:0000303|PubMed:22136599},
GN LIS-L1 {ECO:0000303|PubMed:22020753}, REL2 {ECO:0000303|Ref.7},
GN TPL {ECO:0000303|PubMed:26601214};
GN OrderedLocusNames=Os08g0162100 {ECO:0000312|EMBL:BAF22969.1},
GN LOC_Os08g06480 {ECO:0000305};
GN ORFNames=OsJ_26138 {ECO:0000312|EMBL:EAZ41606.1},
GN P0577B11.132 {ECO:0000312|EMBL:BAC99788.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947 {ECO:0000312|Proteomes:UP000059680};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=22136599; DOI=10.1111/j.1365-313x.2011.04872.x;
RA Yoshida A., Ohmori Y., Kitano H., Taguchi-Shiobara F., Hirano H.;
RT "Aberrant spikelet and panicle1, encoding a TOPLESS-related transcriptional
RT co-repressor, is involved in the regulation of meristem fate in rice.";
RL Plant J. 70:327-339(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP SUBCELLULAR LOCATION, AND GENE FAMILY.
RX PubMed=22020753; DOI=10.1007/s00425-011-1532-7;
RA Gao X., Chen Z., Zhang J., Li X., Chen G., Li X., Wu C.;
RT "OsLIS-L1 encoding a lissencephaly type-1-like protein with WD40 repeats is
RT required for plant height and male gametophyte formation in rice.";
RL Planta 235:713-727(2012).
RN [7]
RP FUNCTION.
RX DOI=10.1007/s11816-011-0214-z;
RA Kwon Y., Yu S., Park J., Li Y., Han J.H., Alavilli H., Cho J., Kim T.H.,
RA Jeon J.S., Lee B.;
RT "OsREL2, a rice TOPLESS homolog functions in axillary meristem development
RT in rice inflorescence.";
RL Plant Biotechnol. Rep. 6:213-224(2012).
RN [8]
RP INTERACTION WITH D53, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24336200; DOI=10.1038/nature12870;
RA Jiang L., Liu X., Xiong G., Liu H., Chen F., Wang L., Meng X., Liu G.,
RA Yu H., Yuan Y., Yi W., Zhao L., Ma H., He Y., Wu Z., Melcher K., Qian Q.,
RA Xu H.E., Wang Y., Li J.;
RT "DWARF 53 acts as a repressor of strigolactone signalling in rice.";
RL Nature 504:401-405(2013).
RN [9]
RP INTERACTION WITH WOX1.
RX PubMed=25697101; DOI=10.1016/j.jgg.2014.12.005;
RA Lu Z., Shao G., Xiong J., Jiao Y., Wang J., Liu G., Meng X., Liang Y.,
RA Xiong G., Wang Y., Li J.;
RT "MONOCULM 3, an ortholog of WUSCHEL in rice, is required for tiller bud
RT formation.";
RL J. Genet. Genomics 42:71-78(2015).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH AP2-3/SNB; AP2-1/TOE1
RP AND AP2-2/IDS1.
RX PubMed=26631749; DOI=10.1073/pnas.1521949112;
RA Wang L., Sun S., Jin J., Fu D., Yang X., Weng X., Xu C., Li X., Xiao J.,
RA Zhang Q.;
RT "Coordinated regulation of vegetative and reproductive branching in rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:15504-15509(2015).
RN [11]
RP SUBUNIT, DOMAIN, AND NOMENCLATURE.
RX PubMed=26601214; DOI=10.1126/sciadv.1500107;
RA Ke J., Ma H., Gu X., Thelen A., Brunzelle J.S., Li J., Xu H.E., Melcher K.;
RT "Structural basis for recognition of diverse transcriptional repressors by
RT the TOPLESS family of corepressors.";
RL Sci. Adv. 1:E1500107-E1500107(2015).
RN [12]
RP INTERACTION WITH MOF1.
RX PubMed=32680975; DOI=10.1104/pp.20.00658;
RA Ren D., Rao Y., Yu H., Xu Q., Cui Y., Xia S., Yu X., Liu H., Hu H., Xue D.,
RA Zeng D., Hu J., Zhang G., Gao Z., Zhu L., Zhang Q., Shen L., Guo L.,
RA Qian Q.;
RT "MORE FLORET 1 encodes a MYB transcription factor that regulates spikelet
RT development in rice.";
RL Plant Physiol. 184:251-265(2020).
CC -!- FUNCTION: Transcriptional corepressor involved in branch formation
CC regulation, presumably by suppressing primary branch formation and
CC promoting secondary branch formation (PubMed:22136599, Ref.7,
CC PubMed:24336200). Required for the cell elongation in the first
CC internode and pollen development (PubMed:22020753). Probable downstream
CC regulator of strigolactones signaling important in axillary meristem
CC maintenance (PubMed:22136599, Ref.7, PubMed:24336200). Acts in auxin
CC signaling and is associated with the regulation of histone
CC deacetylation (PubMed:22136599). Essential for the function of miR172
CC microRNA and its target genes in regulating panicle development
CC (PubMed:26631749). {ECO:0000269|PubMed:22020753,
CC ECO:0000269|PubMed:22136599, ECO:0000269|PubMed:26631749,
CC ECO:0000269|Ref.7, ECO:0000305|PubMed:24336200}.
CC -!- SUBUNIT: Tetramer (PubMed:26601214). Interacts with D53, probably via
CC the EAR motifs (PubMed:24336200). Binds to AP2-1/TOE1, AP2-3/SNB and
CC AP2-2/IDS1 (PubMed:26631749). Interacts with WOX1 (PubMed:25697101,
CC PubMed:24336200, PubMed:26631749, PubMed:26601214). Interacts with MOF1
CC (PubMed:32680975). {ECO:0000269|PubMed:24336200,
CC ECO:0000269|PubMed:25697101, ECO:0000269|PubMed:26601214,
CC ECO:0000269|PubMed:26631749, ECO:0000269|PubMed:32680975}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22020753,
CC ECO:0000269|PubMed:22136599}.
CC -!- TISSUE SPECIFICITY: Expressed in stems and panicles. Detected in roots,
CC seeds, leaves and sheath (PubMed:22020753). Expressed in the meristem
CC and lateral organ primordia (PubMed:22136599).
CC {ECO:0000269|PubMed:22020753, ECO:0000269|PubMed:22136599}.
CC -!- DEVELOPMENTAL STAGE: Highest expression in endosperm at 7 days after
CC pollination. {ECO:0000269|PubMed:22020753}.
CC -!- DOMAIN: The N-terminal TOPLESS domain (TPD) (1-210) binds directly to a
CC 12-amino acid LxLxL EAR motif peptide. {ECO:0000269|PubMed:26601214}.
CC -!- DISRUPTION PHENOTYPE: Semi-dwarf, with reduced male fertility and
CC shorter panicles with arrested branches and abnormal spikelets.
CC {ECO:0000269|PubMed:22020753, ECO:0000269|PubMed:22136599,
CC ECO:0000269|PubMed:26631749}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC99788.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EAZ41606.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB638269; BAL44266.1; -; mRNA.
DR EMBL; AP005504; BAC99788.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008214; BAF22969.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT03947.1; -; Genomic_DNA.
DR EMBL; CM000145; EAZ41606.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; Q0J7U6; -.
DR STRING; 4530.OS08T0162100-01; -.
DR PaxDb; Q0J7U6; -.
DR PRIDE; Q0J7U6; -.
DR EnsemblPlants; Os08t0162100-01; Os08t0162100-01; Os08g0162100.
DR Gramene; Os08t0162100-01; Os08t0162100-01; Os08g0162100.
DR eggNOG; KOG0266; Eukaryota.
DR HOGENOM; CLU_003103_1_0_1; -.
DR OMA; RLVHKPF; -.
DR PlantReactome; R-OSA-5632095; Brassinosteroid signaling.
DR PlantReactome; R-OSA-5654828; Strigolactone signaling.
DR PlantReactome; R-OSA-6787011; Jasmonic acid signaling.
DR PlantReactome; R-OSA-6788019; Salicylic acid signaling.
DR PlantReactome; R-OSA-9608575; Reproductive meristem phase change.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR ExpressionAtlas; Q0J7U6; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0010229; P:inflorescence development; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR006595; CTLH_C.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR027728; Topless_fam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR44083; PTHR44083; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00668; CTLH; 1.
DR SMART; SM00667; LisH; 1.
DR SMART; SM00320; WD40; 9.
DR SUPFAM; SSF50978; SSF50978; 2.
DR PROSITE; PS50897; CTLH; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; WD repeat.
FT CHAIN 1..1133
FT /note="Protein TOPLESS-RELATED PROTEIN 2"
FT /id="PRO_0000435822"
FT DOMAIN 4..36
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT DOMAIN 34..92
FT /note="CTLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
FT REPEAT 344..384
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 451..492
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 495..536
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 539..582
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 586..625
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 630..669
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 771..810
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REPEAT 838..876
FT /note="WD 8"
FT /evidence="ECO:0000255"
FT REPEAT 879..919
FT /note="WD 9"
FT /evidence="ECO:0000255"
FT REPEAT 922..961
FT /note="WD 10"
FT /evidence="ECO:0000255"
FT REPEAT 970..1011
FT /note="WD 11"
FT /evidence="ECO:0000255"
FT REPEAT 1015..1054
FT /note="WD 12"
FT /evidence="ECO:0000255"
FT REGION 1102..1133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1133 AA; 124778 MW; 84E02BA8FF102922 CRC64;
MSSLSRELVF LILQFLDEEK FKETVHKLEQ ESAFYFNMKH FEDLVQGGEW DEVEKYLSGF
TKVEDNRYSM KIFFEIRKQK YLEALDRHDR AKAVEILVKD LKVFASFNEE LFKEITQLLT
LENFRQNEQL SKYGDTKSAR NIMLMELKKL IEANPLFRDK LNFPPFKVSR LRTLINQSLN
WQHQLCKNPR PNPDIKTLFT DHSCAAPTNG ARAPPPANGP LVGPIPKSAA FPPMGAHAPF
QPVVSPSPNA IAGWMTNANP SLPHAAVAQG PPGLVQPPNT AAFLKHPRTP TSAPAIDYQS
ADSEHLMKRM RVGQPDEVSF SGASHPANIY TQDDLPKQVV RNLNQGSNVM SLDFHPVQQT
ILLVGTNVGD IGIWEVGSRE RIAHKTFKVW DISSCTLPLQ AALMKDAAIS VNRCLWSPDG
SILGVAFSKH IVQTYAFVLN GELRQQAEID AHIGGVNDIA FSHPNKTLSI ITCGDDKLIK
VWDAQTGQKQ YTFEGHEAPV YSVCPHYKES IQFIFSTAID GKIKAWLYDC LGSRVDYDAP
GHWCTTMAYS ADGTRLFSCG TSKDGDSHLV EWNETEGAIK RTYNGFRKRS LGVVQFDTTR
NRFLAAGDEF VVKFWDMDNT NILTTTDCDG GLPASPRLRF NREGSLLAVT ANENGIKILA
NTDGQRLLRM LESRAYEGSR GPPQQINTKP PIVNTLGSVS NVSSPMAVNS ERPDRALPTV
SMSGLAPMDV SRTPDVKPRI TDESEKVKTW KLADIGDSGH LRALRMPDTS ATSSKVVRLL
YTNNGVALLA LGSNAVHKLW KWQRTDRNPN GKSTASFTPQ MWQPANGILM ANDTSDGNPE
EATACIALSK NDSYVMSASG GKVSLFNMMT FKVMTTFMAP PPAATFLAFH PQDNNIIAIG
MEDSTIQIYN VRVDEVKSKL KGHSKKITGL AFSQSMNMLV SSGADAQLCA WSIDGWEKKK
SRYIQSPANR SGALVGDTRV QFHNDQTHIL VVHESQLAIY DAKLECLRSW SPREALPAPI
SSAIYSCDGL LIYAGFCDGA IGVFEAESLR LRCRIAPSAY IPPSMSSGGS VYPMVVAAHP
LEPNQIAVGM SDGAVHVVEP LDSDPKWGVA PPQDNGTHPT ISAAPAAANK PEV
