TPR3_ARATH
ID TPR3_ARATH Reviewed; 1108 AA.
AC Q84JM4; Q9S9U5;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Topless-related protein 3;
GN Name=TPR3; OrderedLocusNames=At5g27030; ORFNames=F15P11.3, F2P16.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16763149; DOI=10.1126/science.1123841;
RA Long J.A., Ohno C., Smith Z.R., Meyerowitz E.M.;
RT "TOPLESS regulates apical embryonic fate in Arabidopsis.";
RL Science 312:1520-1523(2006).
RN [5]
RP INTERACTION WITH AFPH2.
RX PubMed=20360743; DOI=10.1038/nature08854;
RA Pauwels L., Barbero G.F., Geerinck J., Tilleman S., Grunewald W.,
RA Perez A.C., Chico J.M., Bossche R.V., Sewell J., Gil E., Garcia-Casado G.,
RA Witters E., Inze D., Long J.A., De Jaeger G., Solano R., Goossens A.;
RT "NINJA connects the co-repressor TOPLESS to jasmonate signalling.";
RL Nature 464:788-791(2010).
RN [6]
RP INTERACTION WITH SPEAR3/TIE1.
RX PubMed=23444332; DOI=10.1105/tpc.113.109223;
RA Tao Q., Guo D., Wei B., Zhang F., Pang C., Jiang H., Zhang J., Wei T.,
RA Gu H., Qu L.J., Qin G.;
RT "The TIE1 transcriptional repressor links TCP transcription factors with
RT TOPLESS/TOPLESS-RELATED corepressors and modulates leaf development in
RT Arabidopsis.";
RL Plant Cell 25:421-437(2013).
RN [7]
RP INTERACTION WITH SMXL6.
RX PubMed=22065421; DOI=10.1104/pp.111.186999;
RA Causier B., Ashworth M., Guo W., Davies B.;
RT "The TOPLESS interactome: a framework for gene repression in Arabidopsis.";
RL Plant Physiol. 158:423-438(2012).
RN [8]
RP INTERACTION WITH SPL.
RX PubMed=25527103; DOI=10.1016/j.jgg.2014.08.009;
RA Chen G.H., Sun J.Y., Liu M., Liu J., Yang W.C.;
RT "SPOROCYTELESS is a novel embryophyte-specific transcription repressor that
RT interacts with TPL and TCP proteins in Arabidopsis.";
RL J. Genet. Genomics 41:617-625(2014).
RN [9]
RP INTERACTION WITH SPL.
RX PubMed=25378179; DOI=10.1038/cr.2014.145;
RA Wei B., Zhang J., Pang C., Yu H., Guo D., Jiang H., Ding M., Chen Z.,
RA Tao Q., Gu H., Qu L.J., Qin G.;
RT "The molecular mechanism of sporocyteless/nozzle in controlling Arabidopsis
RT ovule development.";
RL Cell Res. 25:121-134(2015).
RN [10]
RP SUBUNIT, AND DOMAIN.
RX PubMed=26601214; DOI=10.1126/sciadv.1500107;
RA Ke J., Ma H., Gu X., Thelen A., Brunzelle J.S., Li J., Xu H.E., Melcher K.;
RT "Structural basis for recognition of diverse transcriptional repressors by
RT the TOPLESS family of corepressors.";
RL Sci. Adv. 1:E1500107-E1500107(2015).
CC -!- FUNCTION: Transcriptional corepressor. Negative regulator of jasmonate
CC responses (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Tetramer (PubMed:26601214). Interacts with NINJA/AFPH2
CC (PubMed:20360743). Interacts with SMXL6 (PubMed:22065421). Interacts
CC with SPL (via EAR motif) (PubMed:25527103, PubMed:25378179). Interacts
CC with SPEAR3/TIE1 (PubMed:23444332). {ECO:0000269|PubMed:20360743,
CC ECO:0000269|PubMed:22065421, ECO:0000269|PubMed:23444332,
CC ECO:0000269|PubMed:25378179, ECO:0000269|PubMed:25527103,
CC ECO:0000269|PubMed:26601214}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q84JM4-1; Sequence=Displayed;
CC -!- DOMAIN: The N-terminal TOPLESS domain (TPD) (1-209) binds directly to a
CC 12-amino acid LxLxL EAR motif peptide. {ECO:0000269|PubMed:26601214}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD48936.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF160760; AAD48936.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED93642.1; -; Genomic_DNA.
DR EMBL; BT004037; AAO42071.1; -; mRNA.
DR EMBL; BT005165; AAO50698.1; -; mRNA.
DR RefSeq; NP_198055.3; NM_122585.5. [Q84JM4-1]
DR AlphaFoldDB; Q84JM4; -.
DR BioGRID; 18036; 73.
DR DIP; DIP-58587N; -.
DR IntAct; Q84JM4; 5.
DR STRING; 3702.AT5G27030.2; -.
DR iPTMnet; Q84JM4; -.
DR PaxDb; Q84JM4; -.
DR PRIDE; Q84JM4; -.
DR ProteomicsDB; 245205; -. [Q84JM4-1]
DR EnsemblPlants; AT5G27030.1; AT5G27030.1; AT5G27030. [Q84JM4-1]
DR GeneID; 832761; -.
DR Gramene; AT5G27030.1; AT5G27030.1; AT5G27030. [Q84JM4-1]
DR KEGG; ath:AT5G27030; -.
DR Araport; AT5G27030; -.
DR eggNOG; KOG0266; Eukaryota.
DR InParanoid; Q84JM4; -.
DR PhylomeDB; Q84JM4; -.
DR PRO; PR:Q84JM4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q84JM4; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0009791; P:post-embryonic development; IEA:UniProt.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0048608; P:reproductive structure development; IEA:UniProt.
DR GO; GO:0048367; P:shoot system development; IEA:UniProt.
DR Gene3D; 2.130.10.10; -; 4.
DR InterPro; IPR006595; CTLH_C.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR027728; Topless_fam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR PANTHER; PTHR44083; PTHR44083; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00668; CTLH; 1.
DR SMART; SM00667; LisH; 1.
DR SMART; SM00320; WD40; 10.
DR SUPFAM; SSF50998; SSF50998; 2.
DR PROSITE; PS50897; CTLH; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Jasmonic acid signaling pathway; Nucleus;
KW Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; WD repeat.
FT CHAIN 1..1108
FT /note="Topless-related protein 3"
FT /id="PRO_0000394734"
FT DOMAIN 4..36
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT DOMAIN 34..92
FT /note="CTLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
FT REPEAT 343..383
FT /note="WD 1"
FT REPEAT 405..444
FT /note="WD 2"
FT REPEAT 450..491
FT /note="WD 3"
FT REPEAT 493..534
FT /note="WD 4"
FT REPEAT 583..623
FT /note="WD 5"
FT REPEAT 628..667
FT /note="WD 6"
FT REPEAT 751..790
FT /note="WD 7"
FT REPEAT 818..856
FT /note="WD 8"
FT REPEAT 859..899
FT /note="WD 9"
FT REPEAT 902..941
FT /note="WD 10"
FT REPEAT 994..1033
FT /note="WD 11"
FT REGION 706..725
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1084..1108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1090..1108
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 214
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q94AI7"
SQ SEQUENCE 1108 AA; 122657 MW; 8E9AF285707FBBA9 CRC64;
MSSLSRELVF LILQFLEEEK FKESVHRLEK ESGFFFNTKY FDEKVLAGEW DDVETYLSGF
TKVDDNRYSM KIFFEIRKQK YLEALDRQEK AKAVEILVQD LRVFSTFNEE LYKEITQLLT
LQNFRENEQL SKYGDTKTAR GIMLGELKKL IEANPLFRDK LMFPTLRSSR LRTLINQSLN
WQHQLCKNPR PNPDIKTLFT DHTCTLPNGP LAPSAVNQPV TTLTKPAAYP SLGPHVPFPP
GPAAANAGAL ASWMAAASGA SAVQAAVVTP ALMPQPQNQM SILKRPRTPP ATPGIVDYQN
PDHELMKRLR PAPSVEEVTY PAPRQQAPWS LEDLPTKAAL ALHQGSTVTS MEFYPMQNTL
LLVGSATGEI TLWELAARER LVSRPFKIWD MSNCSHQFQA LIAKETPISV TRVAWSPDGN
FIGVAFTKHL IQLYAFSGPN DLRQHTEIDA HVGAVNDLAF ANPNRQLCVI TCGDDKLIKV
WDVSGRKHFT FEGHDAPVYS ICPHYKENIQ FIFSTAIDGK IKAWLYDNLG SRVDYDAPGK
WCTRMLYSAD GTRLFSCGTS KDGDSFLVEW NESEGSIKRT YKEFQKKLAG VVQFDTSKNH
FLAVGEDGQI KFWDMNNINV LTSTDAEGGL PALPHLRFNK DGNLLAVTTA DNGFKILANP
AGFRSLRAME TPASETMRTP VDFKAVPGAP VASVNCKVER GSPVRHSQML NGVDPSKSRI
DDSTDKPKSW QLAEILDPSQ CFQATLPDTA GSSTKVVQLL YTNSGAGILA LGSNGIQRLW
KWVPNEQNPS GKATATVVPQ HWQPNSGLLM TNDVSGVNLE NAAPCIALSK NDSYVMSAAG
GKVSLFNMMT FKVMTTFMPP PPASTFLAFH PQDNNVIAIG MEDSTIHIYN VRVDEVKSKL
KGHQKRITGL AFSTALNILV SSGADAQICF WSIDTWEKRK SVAIQMPAGK AANGDTRVQF
HVDQLRILVV HETQLAVFDA SKMECIRQWI PQDSLSAPIS SAVYACNSQL IYTTFRDGNI
GVFDADSLRL RCRISPSAYL PQGNQGLSPL VVAAHPQDPN QFAVGLNDGS VKMMEPTEGE
GKWGMIPPSE AINSPSTTSN QTPEQLQR
