TPRA1_HUMAN
ID TPRA1_HUMAN Reviewed; 373 AA.
AC Q86W33; A8MVB8; D3DNA9; Q8WZ24; Q96AJ6; Q9P2R4;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Transmembrane protein adipocyte-associated 1;
DE AltName: Full=Integral membrane protein GPR175;
DE AltName: Full=Transmembrane protein 227;
GN Name=TPRA1; Synonyms=GPR175, TMEM227; ORFNames=PP6566;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Fetal brain;
RA Saito T., Seki N.;
RT "Seven transmembrane domain orphan receptor.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X.,
RA Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.,
RA Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Pancreas, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP TISSUE SPECIFICITY.
RC TISSUE=Adipocyte;
RX PubMed=10342878; DOI=10.1210/endo.140.6.6830;
RA Yang H., Egan J.M., Rodgers B.D., Bernier M., Montrose-Rafizadeh C.;
RT "Differential expression of a novel seven transmembrane domain protein in
RT epididymal fat from aged and diabetic mice.";
RL Endocrinology 140:2859-2867(1999).
CC -!- INTERACTION:
CC Q86W33; Q9NZ94-2: NLGN3; NbExp=3; IntAct=EBI-16424742, EBI-16423037;
CC Q86W33-2; O00155: GPR25; NbExp=3; IntAct=EBI-18976295, EBI-10178951;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q86W33-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q86W33-2; Sequence=VSP_016932, VSP_016933;
CC Name=3;
CC IsoId=Q86W33-3; Sequence=VSP_016934, VSP_016935;
CC -!- TISSUE SPECIFICITY: Ubiquitous, with higher levels in heart, placenta
CC and kidney. {ECO:0000269|PubMed:10342878}.
CC -!- SIMILARITY: Belongs to the UPF0359 family. {ECO:0000305}.
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DR EMBL; AB037108; BAA89782.1; -; mRNA.
DR EMBL; AF289568; AAL55752.1; -; mRNA.
DR EMBL; AC023593; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC084035; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471052; EAW79343.1; -; Genomic_DNA.
DR EMBL; CH471052; EAW79344.1; -; Genomic_DNA.
DR EMBL; BC017058; AAH17058.1; -; mRNA.
DR EMBL; BC050711; AAH50711.1; -; mRNA.
DR CCDS; CCDS3042.1; -. [Q86W33-1]
DR CCDS; CCDS46899.1; -. [Q86W33-3]
DR RefSeq; NP_001129525.1; NM_001136053.2. [Q86W33-1]
DR RefSeq; NP_001136118.1; NM_001142646.2. [Q86W33-3]
DR RefSeq; XP_006713558.1; XM_006713495.3.
DR RefSeq; XP_006713559.1; XM_006713496.2. [Q86W33-1]
DR RefSeq; XP_006713560.1; XM_006713497.3.
DR RefSeq; XP_011510724.1; XM_011512422.1.
DR RefSeq; XP_016861196.1; XM_017005707.1.
DR RefSeq; XP_016861197.1; XM_017005708.1.
DR AlphaFoldDB; Q86W33; -.
DR BioGRID; 126289; 45.
DR IntAct; Q86W33; 25.
DR STRING; 9606.ENSP00000347748; -.
DR GlyGen; Q86W33; 3 sites.
DR iPTMnet; Q86W33; -.
DR PhosphoSitePlus; Q86W33; -.
DR BioMuta; TPRA1; -.
DR DMDM; 74727643; -.
DR jPOST; Q86W33; -.
DR MassIVE; Q86W33; -.
DR MaxQB; Q86W33; -.
DR PaxDb; Q86W33; -.
DR PeptideAtlas; Q86W33; -.
DR PRIDE; Q86W33; -.
DR ProteomicsDB; 70108; -. [Q86W33-1]
DR ProteomicsDB; 70109; -. [Q86W33-2]
DR ProteomicsDB; 70110; -. [Q86W33-3]
DR Antibodypedia; 17215; 292 antibodies from 30 providers.
DR DNASU; 131601; -.
DR Ensembl; ENST00000296210.11; ENSP00000296210.7; ENSG00000163870.16. [Q86W33-3]
DR Ensembl; ENST00000355552.8; ENSP00000347748.3; ENSG00000163870.16. [Q86W33-1]
DR Ensembl; ENST00000393400.6; ENSP00000377056.2; ENSG00000163870.16. [Q86W33-2]
DR Ensembl; ENST00000450633.6; ENSP00000413428.2; ENSG00000163870.16. [Q86W33-1]
DR Ensembl; ENST00000489960.5; ENSP00000418298.1; ENSG00000163870.16. [Q86W33-1]
DR Ensembl; ENST00000648957.1; ENSP00000498161.1; ENSG00000163870.16. [Q86W33-1]
DR GeneID; 131601; -.
DR KEGG; hsa:131601; -.
DR MANE-Select; ENST00000355552.8; ENSP00000347748.3; NM_001136053.4; NP_001129525.1.
DR UCSC; uc003ejn.4; human. [Q86W33-1]
DR CTD; 131601; -.
DR DisGeNET; 131601; -.
DR GeneCards; TPRA1; -.
DR HGNC; HGNC:30413; TPRA1.
DR HPA; ENSG00000163870; Low tissue specificity.
DR MIM; 608336; gene.
DR neXtProt; NX_Q86W33; -.
DR OpenTargets; ENSG00000163870; -.
DR PharmGKB; PA165698543; -.
DR VEuPathDB; HostDB:ENSG00000163870; -.
DR eggNOG; KOG4536; Eukaryota.
DR GeneTree; ENSGT00390000016807; -.
DR HOGENOM; CLU_056255_0_0_1; -.
DR InParanoid; Q86W33; -.
DR OMA; GGMLFWF; -.
DR OrthoDB; 948626at2759; -.
DR PhylomeDB; Q86W33; -.
DR TreeFam; TF314072; -.
DR PathwayCommons; Q86W33; -.
DR SignaLink; Q86W33; -.
DR BioGRID-ORCS; 131601; 12 hits in 1076 CRISPR screens.
DR ChiTaRS; TPRA1; human.
DR GenomeRNAi; 131601; -.
DR Pharos; Q86W33; Tdark.
DR PRO; PR:Q86W33; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q86W33; protein.
DR Bgee; ENSG00000163870; Expressed in lower esophagus mucosa and 154 other tissues.
DR ExpressionAtlas; Q86W33; baseline and differential.
DR Genevisible; Q86W33; HS.
DR GO; GO:0016021; C:integral component of membrane; TAS:ProtInc.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0007568; P:aging; TAS:ProtInc.
DR GO; GO:0040016; P:embryonic cleavage; IEA:Ensembl.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0006629; P:lipid metabolic process; TAS:ProtInc.
DR GO; GO:1901991; P:negative regulation of mitotic cell cycle phase transition; IEA:Ensembl.
DR InterPro; IPR018781; TPRA1/CAND2/CAND8.
DR PANTHER; PTHR15876; PTHR15876; 1.
DR Pfam; PF10160; Tmemb_40; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..373
FT /note="Transmembrane protein adipocyte-associated 1"
FT /id="PRO_0000076099"
FT TRANSMEM 48..68
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT CARBOHYD 11
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 23
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 361
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 204..293
FT /note="VYSLVVILPKTPLKERISLPSRRSFYVYAGILALLNLLQGLGSVLLCFDIIE
FT GLCCVDATTFLYFSFFAPLIYVAFLRGFFGSEPKILFS -> LCRCHNLPVLQLLRSAH
FT LRGFPPGLLRLGAQDPLLLQMPSGRDRGARCTPTPALRCGPAGGPGGCRGCWGLSCQLL
FT EHAVRLCRRGGLPG (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15498874"
FT /id="VSP_016934"
FT VAR_SEQ 204..239
FT /note="VYSLVVILPKTPLKERISLPSRRSFYVYAGILALLN -> LGGASTCMRASW
FT HCSTYCRGWGVCCCASTSSRGSAV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_016932"
FT VAR_SEQ 240..373
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_016933"
FT VAR_SEQ 294..373
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15498874"
FT /id="VSP_016935"
FT CONFLICT 141..142
FT /note="HL -> TW (in Ref. 1; BAA89782)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 373 AA; 41053 MW; DDD489CBB98EAF1A CRC64;
MDTLEEVTWA NGSTALPPPL APNISVPHRC LLLLYEDIGT SRVRYWDLLL LIPNVLFLIF
LLWKLPSARA KIRITSSPIF ITFYILVFVV ALVGIARAVV SMTVSTSNAA TVADKILWEI
TRFFLLAIEL SVIILGLAFG HLESKSSIKR VLAITTVLSL AYSVTQGTLE ILYPDAHLSA
EDFNIYGHGG RQFWLVSSCF FFLVYSLVVI LPKTPLKERI SLPSRRSFYV YAGILALLNL
LQGLGSVLLC FDIIEGLCCV DATTFLYFSF FAPLIYVAFL RGFFGSEPKI LFSYKCQVDE
TEEPDVHLPQ PYAVARREGL EAAGAAGASA ASYSSTQFDS AGGVAYLDDI ASMPCHTGSI
NSTDSERWKA INA
