ID   TPRKB_RAT               Reviewed;         158 AA.
AC   Q5PQR8;
DT   06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=EKC/KEOPS complex subunit Tprkb {ECO:0000305};
DE   AltName: Full=PRPK-binding protein {ECO:0000250|UniProtKB:Q9Y3C4};
DE   AltName: Full=TP53RK-binding protein {ECO:0000250|UniProtKB:Q9Y3C4};
GN   Name=Tprkb {ECO:0000312|RGD:1309786};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of the EKC/KEOPS complex that is required for the
CC       formation of a threonylcarbamoyl group on adenosine at position 37
CC       (t(6)A37) in tRNAs that read codons beginning with adenine. The complex
CC       is probably involved in the transfer of the threonylcarbamoyl moiety of
CC       threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TPRKB acts as an
CC       allosteric effector that regulates the t(6)A activity of the complex.
CC       TPRKB is not required for tRNA modification.
CC       {ECO:0000250|UniProtKB:Q9Y3C4}.
CC   -!- SUBUNIT: Component of the EKC/KEOPS complex composed of at least GON7,
CC       TP53RK, TPRKB, OSGEP and LAGE3; the whole complex dimerizes. Interacts
CC       with TP53RK/PRPK. {ECO:0000250|UniProtKB:Q9Y3C4}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q9Y3C4}. Nucleus {ECO:0000250|UniProtKB:Q9Y3C4}.
CC   -!- SIMILARITY: Belongs to the CGI121/TPRKB family. {ECO:0000305}.
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DR   EMBL; BC087060; AAH87060.1; -; mRNA.
DR   AlphaFoldDB; Q5PQR8; -.
DR   SMR; Q5PQR8; -.
DR   STRING; 10116.ENSRNOP00000021211; -.
DR   PRIDE; Q5PQR8; -.
DR   Ensembl; ENSRNOT00000078239; ENSRNOP00000074558; ENSRNOG00000050645.
DR   UCSC; RGD:1309786; rat.
DR   RGD; 1309786; Tprkb.
DR   eggNOG; KOG4066; Eukaryota.
DR   GeneTree; ENSGT00390000012942; -.
DR   InParanoid; Q5PQR8; -.
DR   PhylomeDB; Q5PQR8; -.
DR   PRO; PR:Q5PQR8; -.
DR   Proteomes; UP000002494; Chromosome 4.
DR   Bgee; ENSRNOG00000050645; Expressed in kidney and 19 other tissues.
DR   ExpressionAtlas; Q5PQR8; baseline and differential.
DR   Genevisible; Q5PQR8; RN.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0000408; C:EKC/KEOPS complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0019901; F:protein kinase binding; ISO:RGD.
DR   GO; GO:0000722; P:telomere maintenance via recombination; IBA:GO_Central.
DR   GO; GO:0002949; P:tRNA threonylcarbamoyladenosine modification; ISS:UniProtKB.
DR   Gene3D; 3.30.2380.10; -; 1.
DR   InterPro; IPR013926; CGI121/TPRKB.
DR   InterPro; IPR036504; CGI121/TPRKB_sf.
DR   PANTHER; PTHR15840; PTHR15840; 1.
DR   Pfam; PF08617; CGI-121; 1.
DR   SUPFAM; SSF143870; SSF143870; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Reference proteome; tRNA processing.
FT   CHAIN           1..158
FT                   /note="EKC/KEOPS complex subunit Tprkb"
FT                   /id="PRO_0000279222"
SQ   SEQUENCE   158 AA;  17593 MW;  6F0A595BB3549F81 CRC64;
     MQLTQQLDLF PECKVTLLLF KDVKNAGDLR KKAMEGSIDG SLINANVIVD PFQILVAANK
     AVHLHKLGKM KTRTLSTEII FNLSPNNNIS EALKKFGISE SNTSVLIVYV EDGDKQVHQE
     HLVSQVEGQQ VPLESLPEIT RLSEVRKVCS TQPEEVSR