TPRN_MOUSE
ID TPRN_MOUSE Reviewed; 749 AA.
AC A2AI08; Q3TAJ8; Q3TMR8; Q5FWB3; Q7TNG3; Q8BNM5; Q8R1G4;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Taperin;
GN Name=Tprn;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Diencephalon;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 135-749.
RC STRAIN=FVB/N; TISSUE=Brain, and Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-457 AND SER-501, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [5]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=20170899; DOI=10.1016/j.ajhg.2010.01.030;
RA Rehman A.U., Morell R.J., Belyantseva I.A., Khan S.Y., Boger E.T.,
RA Shahzad M., Ahmed Z.M., Riazuddin S., Khan S.N., Riazuddin S.,
RA Friedman T.B.;
RT "Targeted capture and next-generation sequencing identifies C9orf75,
RT encoding taperin, as the mutated gene in nonsyndromic deafness DFNB79.";
RL Am. J. Hum. Genet. 86:378-388(2010).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=20170898; DOI=10.1016/j.ajhg.2010.02.003;
RA Li Y., Pohl E., Boulouiz R., Schraders M., Nurnberg G., Charif M.,
RA Admiraal R.J., von Ameln S., Baessmann I., Kandil M., Veltman J.A.,
RA Nurnberg P., Kubisch C., Barakat A., Kremer H., Wollnik B.;
RT "Mutations in TPRN cause a progressive form of autosomal-recessive
RT nonsyndromic hearing loss.";
RL Am. J. Hum. Genet. 86:479-484(2010).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-457 AND SER-501, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Cell projection, stereocilium
CC {ECO:0000269|PubMed:20170899}. Note=Localized prominently at the taper
CC regions of hair cell stereocilia.
CC -!- TISSUE SPECIFICITY: Expressed in the sensory epithelia of the organ of
CC Corti and vestibular end organs and, to a lesser extent, in Reisner's
CC membrane and the spiral ligament (at protein level). At postnatal day
CC 2, expression is detected in cochlea, liver, brain, kidney, heart and
CC lung. {ECO:0000269|PubMed:20170898, ECO:0000269|PubMed:20170899}.
CC -!- SIMILARITY: Belongs to the taperin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH89508.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC38669.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC38669.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAE38373.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAE42670.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAE42670.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK082888; BAC38669.1; ALT_SEQ; mRNA.
DR EMBL; AK165772; BAE38373.1; ALT_INIT; mRNA.
DR EMBL; AK171799; BAE42670.1; ALT_SEQ; mRNA.
DR EMBL; AL732309; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC024573; AAH24573.1; -; mRNA.
DR EMBL; BC055478; AAH55478.1; -; mRNA.
DR EMBL; BC089508; AAH89508.1; ALT_INIT; mRNA.
DR CCDS; CCDS15759.2; -.
DR RefSeq; NP_780495.2; NM_175286.4.
DR AlphaFoldDB; A2AI08; -.
DR STRING; 10090.ENSMUSP00000109975; -.
DR iPTMnet; A2AI08; -.
DR PhosphoSitePlus; A2AI08; -.
DR EPD; A2AI08; -.
DR MaxQB; A2AI08; -.
DR PaxDb; A2AI08; -.
DR PeptideAtlas; A2AI08; -.
DR PRIDE; A2AI08; -.
DR ProteomicsDB; 259072; -.
DR Antibodypedia; 18953; 40 antibodies from 13 providers.
DR Ensembl; ENSMUST00000114336; ENSMUSP00000109975; ENSMUSG00000048707.
DR GeneID; 97031; -.
DR KEGG; mmu:97031; -.
DR UCSC; uc008ira.2; mouse.
DR CTD; 286262; -.
DR MGI; MGI:2139535; Tprn.
DR VEuPathDB; HostDB:ENSMUSG00000048707; -.
DR eggNOG; ENOG502RHCM; Eukaryota.
DR GeneTree; ENSGT00530000064035; -.
DR HOGENOM; CLU_023685_1_0_1; -.
DR InParanoid; A2AI08; -.
DR OMA; KAKVPCS; -.
DR OrthoDB; 732540at2759; -.
DR PhylomeDB; A2AI08; -.
DR TreeFam; TF333324; -.
DR BioGRID-ORCS; 97031; 1 hit in 73 CRISPR screens.
DR PRO; PR:A2AI08; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; A2AI08; protein.
DR Bgee; ENSMUSG00000048707; Expressed in seminiferous tubule of testis and 148 other tissues.
DR Genevisible; A2AI08; MM.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0032420; C:stereocilium; IDA:UniProtKB.
DR GO; GO:0120044; C:stereocilium base; IDA:MGI.
DR GO; GO:0019902; F:phosphatase binding; IEA:InterPro.
DR GO; GO:0060088; P:auditory receptor cell stereocilium organization; IGI:MGI.
DR GO; GO:0007605; P:sensory perception of sound; IMP:MGI.
DR GO; GO:0120045; P:stereocilium maintenance; IMP:MGI.
DR InterPro; IPR025903; Phostensin/Taperin_N_dom.
DR InterPro; IPR025907; Phostensin/Taperin_PP1-bd_dom.
DR InterPro; IPR026671; PPP1R18/Tprn.
DR InterPro; IPR033359; TPRN.
DR PANTHER; PTHR21685; PTHR21685; 1.
DR PANTHER; PTHR21685:SF1; PTHR21685:SF1; 1.
DR Pfam; PF13914; Phostensin; 1.
DR Pfam; PF13916; Phostensin_N; 1.
PE 1: Evidence at protein level;
KW Cell projection; Hearing; Phosphoprotein; Reference proteome.
FT CHAIN 1..749
FT /note="Taperin"
FT /id="PRO_0000330310"
FT REGION 144..348
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 502..586
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 636..673
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 730..749
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 167..184
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 249..270
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 290..316
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..339
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 534..548
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 555..586
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 644..667
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 274
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4KMQ1"
FT MOD_RES 401
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4KMQ1"
FT MOD_RES 457
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 501
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT CONFLICT 264
FT /note="P -> T (in Ref. 1; BAE38373)"
FT /evidence="ECO:0000305"
FT CONFLICT 265
FT /note="T -> A (in Ref. 1; BAE42670)"
FT /evidence="ECO:0000305"
FT CONFLICT 432
FT /note="A -> V (in Ref. 1; BAE42670)"
FT /evidence="ECO:0000305"
FT CONFLICT 527
FT /note="A -> D (in Ref. 1; BAE42670)"
FT /evidence="ECO:0000305"
FT CONFLICT 531..532
FT /note="HT -> DN (in Ref. 1; BAE42670)"
FT /evidence="ECO:0000305"
FT CONFLICT 650
FT /note="Missing (in Ref. 1; BAE42670)"
FT /evidence="ECO:0000305"
FT CONFLICT 671
FT /note="E -> G (in Ref. 3; AAH89508)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 749 AA; 80089 MW; 02E3D12D60051082 CRC64;
MAGLGRLDPG PRTVMPAWKR EILERRRAKL AALSGGQGSG AAPDGPNERL VLAESLGPLS
QNPFMRLESE RRRGTRPAQQ LLELYCRVPG VRTIRADNIL IIESAPGFPP AVPPAAGIRA
AEVVVYEAPQ PGRVSRLLEK FDSPAAPCRR GSPERFRPAL PQLPVASASA ATRTPTNRSL
APASPVRLSQ PAPPISPVPV AQRAGQRSAC CEPAHPDGTA GPGARRSDFL QKTGSNSFTV
HPRGLPRSAV NRSLSNGPMT QESPTGPANG LSGSPPVPGK WKPKVESKEP SLHPPPSPGT
PSATSVGPPA FPAPSPASAT PSQRQWVSSA TSANDSFEIR PSSKPDMETI PIGDLQARAL
ANLRVNSRNS FVLIPKRKAP GNYPLAGRQF EEPKGEVGWA SQSQGLGSQL VSTVDGAPAL
EKSPLAAEMQ WAVRKGACPR PAISDTDKCV RWQRPASPPP FLPATAEAEP AEGLGVPGLA
KNGQEPVRPG LPVTFIDEVD SEEEAFQEAK LPSSAVGVPS QYHLHPARPG HTSELLNRGS
NTFTVVPKRK PGTLQEPHLS QTNGQSQQGA EEQDAESLSG PHTTLENTLK KRYPTVHEIE
VIGGYLALQK SCLIKAGSSR KKMKISFNDK SLHTTFEYPS ESSLAQEEAE EEEEEEGEED
GEEEEVGPDS EKPFTVFLPR ATFVSSVGPE SSSGLSSYTP KHSMAFSKWQ EQTLVQTPTD
VELPPKEVML TPASQNDLSD FRSEPALYF
