TPS10_ARATH
ID TPS10_ARATH Reviewed; 861 AA.
AC O80738; Q0WP88;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 10;
DE EC=2.4.1.15;
DE AltName: Full=Trehalose-6-phosphate synthase 10;
DE Short=AtTPS10;
GN Name=TPS10; Synonyms=TPS3; OrderedLocusNames=At1g60140; ORFNames=T13D8.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Kim Y.S., Lee E.J., Kim J.S., Kim C.K., Lim D.H., Chung C.H.;
RT "Isolation and characterization of Arabidopsis trehalose-6-phosphate
RT synthase.";
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-
CC trehalose 6-phosphate + H(+) + UDP; Xref=Rhea:RHEA:18889,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58429,
CC ChEBI:CHEBI:58885, ChEBI:CHEBI:61548; EC=2.4.1.15;
CC -!- SIMILARITY: In the N-terminal section; belongs to the
CC glycosyltransferase 20 family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the trehalose
CC phosphatase family. {ECO:0000305}.
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DR EMBL; AF155151; AAO15312.1; -; mRNA.
DR EMBL; AC004473; AAC24048.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33662.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60414.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60415.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60417.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60418.1; -; Genomic_DNA.
DR EMBL; AK229191; BAF01061.1; -; mRNA.
DR PIR; T02267; T02267.
DR RefSeq; NP_001322702.1; NM_001333886.1.
DR RefSeq; NP_001322703.1; NM_001333885.1.
DR RefSeq; NP_001322705.1; NM_001333882.1.
DR RefSeq; NP_001322706.1; NM_001333884.1.
DR RefSeq; NP_176221.1; NM_104705.4.
DR AlphaFoldDB; O80738; -.
DR SMR; O80738; -.
DR IntAct; O80738; 1.
DR STRING; 3702.AT1G60140.1; -.
DR CAZy; GT20; Glycosyltransferase Family 20.
DR iPTMnet; O80738; -.
DR PaxDb; O80738; -.
DR PRIDE; O80738; -.
DR ProteomicsDB; 232493; -.
DR EnsemblPlants; AT1G60140.1; AT1G60140.1; AT1G60140.
DR EnsemblPlants; AT1G60140.2; AT1G60140.2; AT1G60140.
DR EnsemblPlants; AT1G60140.4; AT1G60140.4; AT1G60140.
DR EnsemblPlants; AT1G60140.5; AT1G60140.5; AT1G60140.
DR EnsemblPlants; AT1G60140.6; AT1G60140.6; AT1G60140.
DR GeneID; 842309; -.
DR Gramene; AT1G60140.1; AT1G60140.1; AT1G60140.
DR Gramene; AT1G60140.2; AT1G60140.2; AT1G60140.
DR Gramene; AT1G60140.4; AT1G60140.4; AT1G60140.
DR Gramene; AT1G60140.5; AT1G60140.5; AT1G60140.
DR Gramene; AT1G60140.6; AT1G60140.6; AT1G60140.
DR KEGG; ath:AT1G60140; -.
DR Araport; AT1G60140; -.
DR TAIR; locus:2195678; AT1G60140.
DR eggNOG; KOG1050; Eukaryota.
DR HOGENOM; CLU_002351_3_1_1; -.
DR InParanoid; O80738; -.
DR OrthoDB; 772297at2759; -.
DR PhylomeDB; O80738; -.
DR PRO; PR:O80738; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O80738; baseline and differential.
DR Genevisible; O80738; AT.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0005992; P:trehalose biosynthetic process; IBA:GO_Central.
DR GO; GO:0070413; P:trehalose metabolism in response to stress; IBA:GO_Central.
DR CDD; cd03788; GT20_TPS; 1.
DR Gene3D; 3.40.50.1000; -; 2.
DR InterPro; IPR001830; Glyco_trans_20.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006379; HAD-SF_hydro_IIB.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR003337; Trehalose_PPase.
DR PANTHER; PTHR10788; PTHR10788; 1.
DR Pfam; PF00982; Glyco_transf_20; 1.
DR Pfam; PF02358; Trehalose_PPase; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR TIGRFAMs; TIGR00685; T6PP; 1.
PE 2: Evidence at transcript level;
KW Glycosyltransferase; Phosphoprotein; Reference proteome; Transferase.
FT CHAIN 1..861
FT /note="Probable alpha,alpha-trehalose-phosphate synthase
FT [UDP-forming] 10"
FT /id="PRO_0000324831"
FT REGION 59..546
FT /note="Glycosyltransferase"
FT MOD_RES 5
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O23617"
FT MOD_RES 32
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O23617"
FT CONFLICT 412
FT /note="V -> E (in Ref. 4; BAF01061)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 861 AA; 97324 MW; E00172C50669A7BA CRC64;
MGSKSFGNLL DLASGDLLDI PQTPRYLPRV MTVPGIISDV DGYGISDGDS DVISLPCRER
KIIVANFLPL NGKKDSETGK WKFSLDNDSP LLHLKDGFSP ETEVIYVGSL KTHVDVSEQD
EVSHNLFEEF NCVATFLPQD VHKKFYLGFC KQQLWPLFHY MLPMCPDHGE RFDRGLWQAY
VSANKIFADK VMGVINLEED YIWIHDYHLM VLPTFLRRRF HRVKLGFFLH SPFPSSEIYR
TLPVREELLR GLLNCDLIGF HTFDYARHFL SCCCRMLGLE YESKRGHIAL DYLGRTVFLK
ILPIGIHMGR LESVLNLPAT AEKLKEIQEK YRGKKIILGV DDMDIFKGLS LKILAFEHLL
QQYPSMLGKI VLIQIVNPAR GSGKDVQEAR KETYDTVKRI NERYGSHDYE PVVLIDRPVP
RFEKSAYYAL AECCIVNAVR DGMNLVPYKY TVCRQGTPSM NKSLGVSDDL PRTSTLVLSE
FIGCSPSLSG AIRVNPWDVD AVADSLYSAI TMSDFEKQLR HKKHFHYIST HDVGYWARSF
SQDLERASRD HYSKRCWGVG WGLGFRLVAL SPNFRRLSIE QTVSAYRRSS KRAIFLDYDG
TLVPETSIVK DPSAEVISAL KALCSDPNNT IFIVSGRGKV SLSEWLAPCE NLGIAAEHGY
FTRWNKSSDW ETSGLSDDLE WKKVVEPIMR LYTETTDGSN IEAKESALVW HHQDADPDFG
SCQAKELLDH LETVLVNEPV IVNRGHQIVE VKPQGVSKGL VTGKILSRML EDGIAPDFVV
CIGDDRSDEE MFENISTTLS AQSSSMSTEI FACTVGRKPS KAKYFLDEVS DVVKLLQGLA
NTSSPKPRYP SHLRVSFESV V
