TPS13_RICCO
ID TPS13_RICCO Reviewed; 572 AA.
AC B9RXW4;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=Probable terpene synthase 13;
DE Short=RcSeTPS13;
DE EC=4.2.3.-;
GN Name=TPS13; ORFNames=RCOM_0906740;
OS Ricinus communis (Castor bean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Euphorbiaceae; Acalyphoideae; Acalypheae;
OC Ricinus.
OX NCBI_TaxID=3988;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Hale;
RX PubMed=20729833; DOI=10.1038/nbt.1674;
RA Chan A.P., Crabtree J., Zhao Q., Lorenzi H., Orvis J., Puiu D.,
RA Melake-Berhan A., Jones K.M., Redman J., Chen G., Cahoon E.B., Gedil M.,
RA Stanke M., Haas B.J., Wortman J.R., Fraser-Liggett C.M., Ravel J.,
RA Rabinowicz P.D.;
RT "Draft genome sequence of the oilseed species Ricinus communis.";
RL Nat. Biotechnol. 28:951-956(2010).
RN [2]
RP GENE NAME.
RX PubMed=22459969; DOI=10.1016/j.phytochem.2012.02.022;
RA Xie X., Kirby J., Keasling J.D.;
RT "Functional characterization of four sesquiterpene synthases from Ricinus
RT communis (castor bean).";
RL Phytochemistry 78:20-28(2012).
CC -!- FUNCTION: Probable sesquiterpene synthase. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250};
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: Does not produce any detectable product when tested in
CC vitro. {ECO:0000305|PubMed:22459969}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR EMBL; EQ973828; EEF43970.1; -; Genomic_DNA.
DR RefSeq; XP_002518583.1; XM_002518537.2.
DR AlphaFoldDB; B9RXW4; -.
DR SMR; B9RXW4; -.
DR STRING; 3988.XP_002518583.1; -.
DR PRIDE; B9RXW4; -.
DR GeneID; 8267425; -.
DR KEGG; rcu:8267425; -.
DR eggNOG; ENOG502QTGK; Eukaryota.
DR InParanoid; B9RXW4; -.
DR OrthoDB; 449049at2759; -.
DR Proteomes; UP000008311; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR GO; GO:0120251; P:hydrocarbon biosynthetic process; IEA:UniProt.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 3: Inferred from homology;
KW Lyase; Magnesium; Metal-binding; Reference proteome.
FT CHAIN 1..572
FT /note="Probable terpene synthase 13"
FT /id="PRO_0000422211"
FT MOTIF 326..330
FT /note="DDXXD motif"
FT BINDING 326
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 326
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 330
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 330
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 478
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
SQ SEQUENCE 572 AA; 65858 MW; 0F0E0B26B423A052 CRC64;
MALSSISIFI HCLVDPLPRK QTSKTLPSNH IDFKSAFNVA NWSINQTLVS PLSAQCIHLT
DEDVRIKERR TRAFKHILRK EGEGSHEVLA MIDAIQRLGI DHHFQDEIDE ILQRQYTIPS
YYNDNDLHGL ALRFRLLRQG GYNVSAGVFD KFKDKEGNFD QKLSDDIRGL MELYEASQLS
IGAEDHILDE AGDYSHQLLS SWMTRLDDSQ ARIIKNTLDH PHHKNLARFR ATNFNRYFHM
ANIEGWMNEL QELAKIDFQM VQSQNQQEIF QVAGWWKDLG ISKELKFVRN QPLKWYIWSM
ATLSDPSLSQ QRIDLTKPIS FIYIIDDIFD VQGSLDELTL FTEIVKRWDV EAVEQLPGYM
RACFKALDSV TNEIGYKVYK QHGWNPVHSL RETWASLCKA FLVEARWFAS GHLPAAEEYL
QNGIVSSGVH VVLVHIFYLL GHGVTREGVD FIGNRPAIIT STATILRLWD DLGISKDENQ
DGHDGSYVEC YVKEHKGSLV EIATKKVTVM ISDAWKQLNQ ECLHPNPFSP NFTKSCLNLA
RMVPLMYSYD DNHRLPVLEY YTKSLLFESV SI