ID   TPS13_RICCO             Reviewed;         572 AA.
AC   B9RXW4;
DT   01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Probable terpene synthase 13;
DE            Short=RcSeTPS13;
DE            EC=4.2.3.-;
GN   Name=TPS13; ORFNames=RCOM_0906740;
OS   Ricinus communis (Castor bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Acalyphoideae; Acalypheae;
OC   Ricinus.
OX   NCBI_TaxID=3988;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Hale;
RX   PubMed=20729833; DOI=10.1038/nbt.1674;
RA   Chan A.P., Crabtree J., Zhao Q., Lorenzi H., Orvis J., Puiu D.,
RA   Melake-Berhan A., Jones K.M., Redman J., Chen G., Cahoon E.B., Gedil M.,
RA   Stanke M., Haas B.J., Wortman J.R., Fraser-Liggett C.M., Ravel J.,
RA   Rabinowicz P.D.;
RT   "Draft genome sequence of the oilseed species Ricinus communis.";
RL   Nat. Biotechnol. 28:951-956(2010).
RN   [2]
RP   GENE NAME.
RX   PubMed=22459969; DOI=10.1016/j.phytochem.2012.02.022;
RA   Xie X., Kirby J., Keasling J.D.;
RT   "Functional characterization of four sesquiterpene synthases from Ricinus
RT   communis (castor bean).";
RL   Phytochemistry 78:20-28(2012).
CC   -!- FUNCTION: Probable sesquiterpene synthase. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250};
CC   -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC       the catalytic activity, presumably through binding to Mg(2+).
CC       {ECO:0000250}.
CC   -!- MISCELLANEOUS: Does not produce any detectable product when tested in
CC       vitro. {ECO:0000305|PubMed:22459969}.
CC   -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR   EMBL; EQ973828; EEF43970.1; -; Genomic_DNA.
DR   RefSeq; XP_002518583.1; XM_002518537.2.
DR   AlphaFoldDB; B9RXW4; -.
DR   SMR; B9RXW4; -.
DR   STRING; 3988.XP_002518583.1; -.
DR   PRIDE; B9RXW4; -.
DR   GeneID; 8267425; -.
DR   KEGG; rcu:8267425; -.
DR   eggNOG; ENOG502QTGK; Eukaryota.
DR   InParanoid; B9RXW4; -.
DR   OrthoDB; 449049at2759; -.
DR   Proteomes; UP000008311; Unassembled WGS sequence.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR   GO; GO:0120251; P:hydrocarbon biosynthetic process; IEA:UniProt.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   3: Inferred from homology;
KW   Lyase; Magnesium; Metal-binding; Reference proteome.
FT   CHAIN           1..572
FT                   /note="Probable terpene synthase 13"
FT                   /id="PRO_0000422211"
FT   MOTIF           326..330
FT                   /note="DDXXD motif"
FT   BINDING         326
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         326
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         330
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         330
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         478
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   572 AA;  65858 MW;  0F0E0B26B423A052 CRC64;
     MALSSISIFI HCLVDPLPRK QTSKTLPSNH IDFKSAFNVA NWSINQTLVS PLSAQCIHLT
     DEDVRIKERR TRAFKHILRK EGEGSHEVLA MIDAIQRLGI DHHFQDEIDE ILQRQYTIPS
     YYNDNDLHGL ALRFRLLRQG GYNVSAGVFD KFKDKEGNFD QKLSDDIRGL MELYEASQLS
     IGAEDHILDE AGDYSHQLLS SWMTRLDDSQ ARIIKNTLDH PHHKNLARFR ATNFNRYFHM
     ANIEGWMNEL QELAKIDFQM VQSQNQQEIF QVAGWWKDLG ISKELKFVRN QPLKWYIWSM
     ATLSDPSLSQ QRIDLTKPIS FIYIIDDIFD VQGSLDELTL FTEIVKRWDV EAVEQLPGYM
     RACFKALDSV TNEIGYKVYK QHGWNPVHSL RETWASLCKA FLVEARWFAS GHLPAAEEYL
     QNGIVSSGVH VVLVHIFYLL GHGVTREGVD FIGNRPAIIT STATILRLWD DLGISKDENQ
     DGHDGSYVEC YVKEHKGSLV EIATKKVTVM ISDAWKQLNQ ECLHPNPFSP NFTKSCLNLA
     RMVPLMYSYD DNHRLPVLEY YTKSLLFESV SI