TPS15_ARATH
ID TPS15_ARATH Reviewed; 601 AA.
AC Q9LS76;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Terpenoid synthase 15;
DE Short=AtTPS15;
DE EC=4.2.3.-;
GN Name=TPS15; OrderedLocusNames=At3g29190; ORFNames=MXO21.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12207221; DOI=10.1007/s00438-002-0709-y;
RA Aubourg S., Lecharny A., Bohlmann J.;
RT "Genomic analysis of the terpenoid synthase (AtTPS) gene family of
RT Arabidopsis thaliana.";
RL Mol. Genet. Genomics 267:730-745(2002).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=12566586; DOI=10.1105/tpc.007989;
RA Chen F., Tholl D., D'Auria J.C., Farooq A., Pichersky E., Gershenzon J.;
RT "Biosynthesis and emission of terpenoid volatiles from Arabidopsis
RT flowers.";
RL Plant Cell 15:481-494(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=12777052; DOI=10.1023/a:1023005504702;
RA Lange B.M., Ghassemian M.;
RT "Genome organization in Arabidopsis thaliana: a survey for genes involved
RT in isoprenoid and chlorophyll metabolism.";
RL Plant Mol. Biol. 51:925-948(2003).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in siliques but also in
CC stems. {ECO:0000269|PubMed:12566586}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01815.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB026657; BAB01815.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002686; AEE77550.1; -; Genomic_DNA.
DR RefSeq; NP_189564.2; NM_113843.3.
DR AlphaFoldDB; Q9LS76; -.
DR SMR; Q9LS76; -.
DR BioGRID; 7901; 1.
DR PaxDb; Q9LS76; -.
DR PRIDE; Q9LS76; -.
DR ProteomicsDB; 228380; -.
DR EnsemblPlants; AT3G29190.1; AT3G29190.1; AT3G29190.
DR GeneID; 822572; -.
DR Gramene; AT3G29190.1; AT3G29190.1; AT3G29190.
DR KEGG; ath:AT3G29190; -.
DR Araport; AT3G29190; -.
DR TAIR; locus:2094827; AT3G29190.
DR HOGENOM; CLU_003125_7_2_1; -.
DR InParanoid; Q9LS76; -.
DR OMA; HATIKEC; -.
DR OrthoDB; 20156at2759; -.
DR PhylomeDB; Q9LS76; -.
DR BioCyc; ARA:AT3G29190-MON; -.
DR UniPathway; UPA00213; -.
DR PRO; PR:Q9LS76; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LS76; baseline and differential.
DR Genevisible; Q9LS76; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009975; F:cyclase activity; IBA:GO_Central.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR GO; GO:0051762; P:sesquiterpene biosynthetic process; IBA:GO_Central.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Lyase; Magnesium; Manganese; Metal-binding; Reference proteome.
FT CHAIN 1..601
FT /note="Terpenoid synthase 15"
FT /id="PRO_0000403706"
FT MOTIF 353..357
FT /note="DDXXD motif"
FT BINDING 353
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 353
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 357
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 357
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 498
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 506
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
SQ SEQUENCE 601 AA; 69668 MW; 5A1C2A916AD51663 CRC64;
MEVISIFGPK HGSLISLHSS YNLFKVSKVT RFRRTLFPSN HAKVFRFKAL TCDNQESNNM
FKELPTSEWT HHFHSIQVDV SEMDAIRIEI DALKPKVKNI LMSFQGIDST KKRILMIYML
ISLGLACQFE EEIYETLKEG FGKIEEMMAN EEDLYTVSII FWVFRRYGHY ISSDFFRRFK
GNDGNFKKSL IGDAKGMLSF YEAANMATTK DYILDEALSF TSSHLESLAA NGACPPHMSR
RIRNALNASQ HWNMEMLVAV EYISFYEKEK DHNEMLLKFS KLNFKFLQLQ YLQELKVLTK
WYKEVDFVSK LPPYFRDRIV ENHFFIQTLF VESQHSRARI MMAKYFILLV IQDDTLDRYA
SLPEAESLVN SLNRWAPDHA MDKQPDYLKF VFKFILDTFE EFEKELRPEG GSFGVCATIE
EFKSLVKANL EAEKWALADN MPSFEEYIEV TGVGITAMTT LMGAMMCMGK IVPKEDYKWL
KSRPKIIQAL AIKGRLMNDM KGYKEDMSRG YAANAVTCYM KQYRVTEQEA LKEFEKMVAV
ANKTVNEEFL TTMGVSRLVL KLAMGVGLMI SITYSEDEGY THPEGKIKEK MTTLFVDQIP
L
