TPS16_ARATH
ID TPS16_ARATH Reviewed; 595 AA.
AC Q9LVP7; F4J1T1;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Putative terpenoid synthase 16;
DE Short=AtTPS16;
DE EC=4.2.3.-;
GN Name=TPS16; OrderedLocusNames=At3g29110; ORFNames=MXE2.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12207221; DOI=10.1007/s00438-002-0709-y;
RA Aubourg S., Lecharny A., Bohlmann J.;
RT "Genomic analysis of the terpenoid synthase (AtTPS) gene family of
RT Arabidopsis thaliana.";
RL Mol. Genet. Genomics 267:730-745(2002).
RN [4]
RP GENE FAMILY.
RX PubMed=12777052; DOI=10.1023/a:1023005504702;
RA Lange B.M., Ghassemian M.;
RT "Genome organization in Arabidopsis thaliana: a survey for genes involved
RT in isoprenoid and chlorophyll metabolism.";
RL Plant Mol. Biol. 51:925-948(2003).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily.
CC {ECO:0000305}.
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DR EMBL; AB018121; BAB01988.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77537.2; -; Genomic_DNA.
DR RefSeq; NP_189555.2; NM_113834.2.
DR AlphaFoldDB; Q9LVP7; -.
DR SMR; Q9LVP7; -.
DR STRING; 3702.AT3G29110.1; -.
DR PaxDb; Q9LVP7; -.
DR PRIDE; Q9LVP7; -.
DR EnsemblPlants; AT3G29110.1; AT3G29110.1; AT3G29110.
DR GeneID; 822559; -.
DR Gramene; AT3G29110.1; AT3G29110.1; AT3G29110.
DR KEGG; ath:AT3G29110; -.
DR Araport; AT3G29110; -.
DR eggNOG; ENOG502SHPY; Eukaryota.
DR HOGENOM; CLU_003125_7_2_1; -.
DR InParanoid; Q9LVP7; -.
DR OMA; EVGHDEM; -.
DR OrthoDB; 360509at2759; -.
DR PhylomeDB; Q9LVP7; -.
DR BioCyc; ARA:AT3G29110-MON; -.
DR UniPathway; UPA00213; -.
DR PRO; PR:Q9LVP7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LVP7; baseline and differential.
DR Genevisible; Q9LVP7; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009975; F:cyclase activity; IBA:GO_Central.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR GO; GO:0051762; P:sesquiterpene biosynthetic process; IBA:GO_Central.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Lyase; Magnesium; Manganese; Metal-binding; Reference proteome.
FT CHAIN 1..595
FT /note="Putative terpenoid synthase 16"
FT /id="PRO_0000403707"
FT MOTIF 349..353
FT /note="DDXXD motif"
FT BINDING 349
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 349
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 353
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 353
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 494
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 502
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
SQ SEQUENCE 595 AA; 68541 MW; 440BA753D5CC6FC2 CRC64;
METITVFGPK HGSPLSLPSR TNMCWEMKPS RFPLTSVRGK PAKQVGLKVS ASCDRPISKL
PPSKWTNYFH SVLVDVSEMD VLEREIEALK PNVREMLMSS KGYDSVKKRS LMIYLLVSLG
LAYHFEEEIE KSLKDGFEKI DEIIAGEDDL YTISTIFWVF RTYGYNMSSD VFRRFKEENG
KFKESLIEDA RGMLSLYEAA HLGTTTDYIL DEALDFASNN LVSLAEDGMC PSHLSTHIRN
ALSISQHWNM EIIVAVQYIR FYEQEVGHDE MLLKFAKLNF NLVQRLYLQE VKILTKWYKD
QDIHSKLPPY YRPVVTEMHF FSTATFFEPQ FSHARILQTK LFMAELLVDD TCDRYATFSE
VESLINSLQR WAPDDAMDTH PDYLKVVFKF ILNAFEECEK ELRPQGRSYS LEQTKEEYKR
FAKSNLDLAK LAQAGNVPSF EEYMEVGKDE IGAFVIVAGS LMGMDNIDAV EAYDFLKSRS
KFSQSSAEIV RYLNDLAGFE DDMRRGCVST GLNCYMNQYG VTETEVFREF RKMVMNTCKI
MNEEFLKTTD VPLRVLKTNF SCVRSGFVGY NEGEGVTYPE GKITKYLTSL YVDQI
