TPS19_ARATH
ID TPS19_ARATH Reviewed; 602 AA.
AC Q9LUE0; Q84UU5;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Terpenoid synthase 19;
DE Short=AtTPS19;
DE EC=4.2.3.-;
GN Name=TPS19; OrderedLocusNames=At3g14540; ORFNames=MIE1.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=12566586; DOI=10.1105/tpc.007989;
RA Chen F., Tholl D., D'Auria J.C., Farooq A., Pichersky E., Gershenzon J.;
RT "Biosynthesis and emission of terpenoid volatiles from Arabidopsis
RT flowers.";
RL Plant Cell 15:481-494(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12207221; DOI=10.1007/s00438-002-0709-y;
RA Aubourg S., Lecharny A., Bohlmann J.;
RT "Genomic analysis of the terpenoid synthase (AtTPS) gene family of
RT Arabidopsis thaliana.";
RL Mol. Genet. Genomics 267:730-745(2002).
RN [5]
RP GENE FAMILY.
RX PubMed=12777052; DOI=10.1023/a:1023005504702;
RA Lange B.M., Ghassemian M.;
RT "Genome organization in Arabidopsis thaliana: a survey for genes involved
RT in isoprenoid and chlorophyll metabolism.";
RL Plant Mol. Biol. 51:925-948(2003).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in flowers and siliques but
CC also in roots and leaves. {ECO:0000269|PubMed:12566586}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily.
CC {ECO:0000305}.
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DR EMBL; AF497490; AAO85538.1; -; mRNA.
DR EMBL; AB023038; BAB02386.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75536.1; -; Genomic_DNA.
DR RefSeq; NP_188072.1; NM_112314.3.
DR AlphaFoldDB; Q9LUE0; -.
DR SMR; Q9LUE0; -.
DR STRING; 3702.AT3G14540.1; -.
DR PaxDb; Q9LUE0; -.
DR PRIDE; Q9LUE0; -.
DR ProteomicsDB; 245214; -.
DR EnsemblPlants; AT3G14540.1; AT3G14540.1; AT3G14540.
DR GeneID; 820680; -.
DR Gramene; AT3G14540.1; AT3G14540.1; AT3G14540.
DR KEGG; ath:AT3G14540; -.
DR Araport; AT3G14540; -.
DR TAIR; locus:2089631; AT3G14540.
DR eggNOG; ENOG502SHPY; Eukaryota.
DR HOGENOM; CLU_003125_7_2_1; -.
DR InParanoid; Q9LUE0; -.
DR OMA; YPISSDY; -.
DR OrthoDB; 360509at2759; -.
DR PhylomeDB; Q9LUE0; -.
DR BioCyc; ARA:AT3G14540-MON; -.
DR UniPathway; UPA00213; -.
DR PRO; PR:Q9LUE0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LUE0; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009975; F:cyclase activity; IBA:GO_Central.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR GO; GO:0051762; P:sesquiterpene biosynthetic process; IBA:GO_Central.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Lyase; Magnesium; Manganese; Metal-binding; Reference proteome.
FT CHAIN 1..602
FT /note="Terpenoid synthase 19"
FT /id="PRO_0000403710"
FT MOTIF 353..357
FT /note="DDXXD motif"
FT BINDING 353
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 353
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 357
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 357
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 497
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 501
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 505
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT CONFLICT 145
FT /note="G -> D (in Ref. 1; AAO85538)"
FT /evidence="ECO:0000305"
FT CONFLICT 396
FT /note="E -> K (in Ref. 1; AAO85538)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 602 AA; 69187 MW; CC0E6384B26F480D CRC64;
MEATRMGFGG LSVPLSLTPN LSLIPQRLLD KHNLSLKPVK IHHLVCVRST KSSDDLETSR
PSTYFSPSLW GDHFLSVSLD HAEFVELERE IETMKPLVKD MLMSSQSSDK EKIRLIHLLV
SLGSSYHFDK EIQDILKHSF TKLDGIIVEE DDLETISIMF EVFRLYGHKM SCDAFDRFRG
GDGRFKESLA KDVRGMLQLF EVAHLGTLSE DIMDEALRFT RNHLESLTSG NVSSASPHIL
KHIQNSLYIP RYCNIEVLVA REYISYYEQE EGYNEILLKF AKLNFNFCQC HYIQEIKTLT
KWWKDLDLAS KLPYIRDRSV ESHLGGLGPY FEPQYSLGRI IVAKTIMIIV VADDTYDAHA
TIPEATVLTE YFQRLNIGAD DKLSGYLRIV LESVFEVMGE IEQEMSPKGR SYSVKQVLER
FKIIAKAYKQ LTEWARKGHV PTFDEYMKVG LVTAGMGDYA GYCFIGMEDI NEKEAFEWLN
SNPLLIDALN VLFRIANDVG TYETEINRGE VANGLNCYMK QYGVTKEEAS RELRKMYIYN
KKVVVEEFMN SHDRVPRQVL LRCLNFARLF DVIYTEGDGY SEPKGKIEHF MTSLYVHPIP
LS
