ID   TPS1_METBS              Reviewed;         331 AA.
AC   A0A0B4G504;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   04-MAR-2015, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=(E)-beta farnesene synthase MBR_03882 {ECO:0000303|PubMed:31239482};
DE            EC=4.2.3.47 {ECO:0000269|PubMed:31239482};
DE   AltName: Full=Typical fungal terpene synthase MBR_03882 {ECO:0000303|PubMed:31239482};
GN   ORFNames=MBR_03882;
OS   Metarhizium brunneum (strain ARSEF 3297).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX   NCBI_TaxID=1276141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ARSEF 3297;
RX   PubMed=25368161; DOI=10.1073/pnas.1412662111;
RA   Hu X., Xiao G., Zheng P., Shang Y., Su Y., Zhang X., Liu X., Zhan S.,
RA   St Leger R.J., Wang C.;
RT   "Trajectory and genomic determinants of fungal-pathogen speciation and host
RT   adaptation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 111:16796-16801(2014).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=31239482; DOI=10.1038/s41598-019-45532-1;
RA   Jia Q., Chen X., Koellner T.G., Rinkel J., Fu J., Labbe J., Xiong W.,
RA   Dickschat J.S., Gershenzon J., Chen F.;
RT   "Terpene synthase genes originated from bacteria through horizontal gene
RT   transfer contribute to terpenoid diversity in fungi.";
RL   Sci. Rep. 9:9223-9223(2019).
CC   -!- FUNCTION: Terpene synthase that catalyzes the conversion of (2E,6E)-
CC       farnesyl diphosphate (FPP) into the volatile sesquiterpene (E)-beta-
CC       farnesene. {ECO:0000269|PubMed:31239482}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6E)-farnesyl diphosphate = (E)-beta-farnesene +
CC         diphosphate; Xref=Rhea:RHEA:27425, ChEBI:CHEBI:10418,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:175763; EC=4.2.3.47;
CC         Evidence={ECO:0000269|PubMed:31239482};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:27426;
CC         Evidence={ECO:0000269|PubMed:31239482};
CC   -!- SIMILARITY: Belongs to the trichodiene synthase family. {ECO:0000305}.
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DR   EMBL; AZNG01000003; KID77563.1; -; Genomic_DNA.
DR   RefSeq; XP_014546729.1; XM_014691243.1.
DR   AlphaFoldDB; A0A0B4G504; -.
DR   SMR; A0A0B4G504; -.
DR   EnsemblFungi; KID77563; KID77563; MBR_03882.
DR   GeneID; 26241152; -.
DR   HOGENOM; CLU_052212_0_0_1; -.
DR   GO; GO:0016838; F:carbon-oxygen lyase activity, acting on phosphates; IEA:InterPro.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR024652; Trichodiene_synth.
DR   Pfam; PF06330; TRI5; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   1: Evidence at protein level;
KW   Lyase.
FT   CHAIN           1..331
FT                   /note="(E)-beta farnesene synthase MBR_03882"
FT                   /id="PRO_0000451045"
SQ   SEQUENCE   331 AA;  38026 MW;  788D6618F9C97EDC CRC64;
     METDSFKRQY ADILRRYLCG ISYQKLSCEY DPSIEETVVQ HFRTLNFPND FLKRMMPIIH
     ASAWIATSTY PFTPRHVQEA IAVYTSLAIA IEDTSKESTH DLKRFQQRLF NRQPQPNLLL
     QAMVDCLVSL RGIYGPFICD MVAKSTAEYI SVCAFEAKYD GTLRPTPSSP DFPYYLRLKT
     GVAEVYAFFA FPEVLYPEEA FLHEYILAVP DISRYFNLGN DLLSFYKESI VADERLNYIY
     NCSRVSNSTP LESIWSTHLA LITCVENIRK TLSASPQMRR NIDQLINGYV MYHFGASRYK
     LSDLGIQEVD ELRAKICCST TVDNGVGAYK H