TPS1_SORBI
ID TPS1_SORBI Reviewed; 541 AA.
AC C5YHH7;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2012, sequence version 2.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Zingiberene synthase;
DE Short=SbTPS1;
DE EC=4.2.3.65;
GN Name=TPS1; OrderedLocusNames=Sb07g004470;
OS Sorghum bicolor (Sorghum) (Sorghum vulgare).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Sorghinae; Sorghum.
OX NCBI_TaxID=4558;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF TYR-369; LEU-401 AND VAL-435,
RP FUNCTION, CATALYTIC ACTIVITY, INDUCTION BY HERBIVORY, AND 3D-STRUCTURE
RP MODELING.
RX PubMed=21880075; DOI=10.1111/j.1365-313x.2011.04771.x;
RA Zhuang X., Koellner T.G., Zhao N., Li G., Jiang Y., Zhu L., Ma J.,
RA Degenhardt J., Chen F.;
RT "Dynamic evolution of herbivore-induced sesquiterpene biosynthesis in
RT sorghum and related grass crops.";
RL Plant J. 69:70-80(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. BTx623;
RX PubMed=19189423; DOI=10.1038/nature07723;
RA Paterson A.H., Bowers J.E., Bruggmann R., Dubchak I., Grimwood J.,
RA Gundlach H., Haberer G., Hellsten U., Mitros T., Poliakov A., Schmutz J.,
RA Spannagl M., Tang H., Wang X., Wicker T., Bharti A.K., Chapman J.,
RA Feltus F.A., Gowik U., Grigoriev I.V., Lyons E., Maher C.A., Martis M.,
RA Narechania A., Otillar R.P., Penning B.W., Salamov A.A., Wang Y., Zhang L.,
RA Carpita N.C., Freeling M., Gingle A.R., Hash C.T., Keller B., Klein P.,
RA Kresovich S., McCann M.C., Ming R., Peterson D.G., Mehboob-ur-Rahman M.,
RA Ware D., Westhoff P., Mayer K.F.X., Messing J., Rokhsar D.S.;
RT "The Sorghum bicolor genome and the diversification of grasses.";
RL Nature 457:551-556(2009).
CC -!- FUNCTION: Sesquiterpene synthase converting farnesyl diphosphate into
CC two major products, zingiberene > beta-sesquiphellandrene, and five
CC minor products, 7-epi-sesquithujene, sesquisabinene A, (E)-alpha-
CC bergamotene, (E)-beta-farnesene and beta-bisabolene. Can also accept
CC geranyl diphosphate as substrate, producing nine monoterpenes, with
CC myrcene, limonene and alpha-terpinolene as the major products.
CC {ECO:0000269|PubMed:21880075}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = alpha-zingiberene +
CC diphosphate; Xref=Rhea:RHEA:28643, ChEBI:CHEBI:10115,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:175763; EC=4.2.3.65;
CC Evidence={ECO:0000269|PubMed:21880075};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Up-regulated by herbivory. {ECO:0000269|PubMed:21880075}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EES13421.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; CM000766; EES13421.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; C5YHH7; -.
DR SMR; C5YHH7; -.
DR eggNOG; ENOG502QUCN; Eukaryota.
DR HOGENOM; CLU_003125_7_2_1; -.
DR InParanoid; C5YHH7; -.
DR UniPathway; UPA00213; -.
DR Proteomes; UP000000768; Chromosome 7.
DR ExpressionAtlas; C5YHH7; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0102884; F:alpha-zingiberene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Lyase; Magnesium; Manganese; Metal-binding; Plant defense;
KW Reference proteome.
FT CHAIN 1..541
FT /note="Zingiberene synthase"
FT /id="PRO_0000418831"
FT MOTIF 295..299
FT /note="DDXXD motif"
FT BINDING 295
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 295
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 299
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 299
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 439
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 443
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 447
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT MUTAGEN 369
FT /note="Y->S: No effect on zingiberene production, but
FT increased production of (E)-alpha-bergamotene and (E)-beta-
FT farnesene. Production of beta-sesquiphellandrene and
FT increased production of (E)-alpha-bergamotene and (E)-beta-
FT farnesene; when associated with V-401 or with V-401 and S-
FT 435."
FT /evidence="ECO:0000269|PubMed:21880075"
FT MUTAGEN 401
FT /note="L->V: Changed product specificity and production of
FT mainly beta-sesquiphellandrene. Production of beta-
FT sesquiphellandrene and increased production of (E)-alpha-
FT bergamotene and (E)-beta-farnesene; when associated with S-
FT 369 or with S-369 and S-435."
FT /evidence="ECO:0000269|PubMed:21880075"
FT MUTAGEN 435
FT /note="V->S: Production of mainly beta-sesquiphellandrene
FT and beta-bisabolene. Production of beta-sesquiphellandrene
FT and increased production of (E)-alpha-bergamotene and (E)-
FT beta-farnesene; when associated with S-369 and V-401."
FT /evidence="ECO:0000269|PubMed:21880075"
SQ SEQUENCE 541 AA; 62891 MW; D0F1194A7433940B CRC64;
MAALQYNCDA GLAKAPTFHP SLWGDFFLTY QPPTAPQHVY MKERAELLKE EVREIVKGTN
ELPKLLDLII TLQRLGLDNH YEIEIDEHLH FIYNSSCDVK DLNLVSLRFY LLRKNGYDVS
SDVFLNFKDK DGNFASDDIR SLLSLYNAAY LRTHGEEVLD EAIIFTRRHL EAALTSLESK
LADEVSLSLQ TPLFRRVRIL ETRNYIPIYE MEPSRNEAML EFAKLNFNLL QILYCEELKT
VTAWWKQLNI ETDLSFIRDR IVEMHFWMAG ACSEPKYSLS RVILTKMTAF ITILDDIIDT
HSTTEEGKLL AKAIDRCSQD ANEVLPDYMK HFYMFLLKTF DSCEDELGPN KRYRVFYLKE
LLKILVRGYS QEIEWRDEHY VPETIDKHLE ISRVTVGAFQ LACSSFVGMG DIITKEVLDW
LLTYPELLKC FTTFVRLSND ITSTKREQTG GHHASTVQCY MMEHSTTMHD ACEKIKGLIE
DSWKDMMQLY LTPTEQSKVV AQTVVDFART GDYMYKKTDA FTFSHTIKDM IALLYVEPIL
F
