TPS1_VITAC
ID TPS1_VITAC Reviewed; 798 AA.
AC A0A2K9RFZ7;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 25-APR-2018, sequence version 1.
DT 03-AUG-2022, entry version 13.
DE RecName: Full=Peregrinol diphosphate synthase TPS1, chloroplastic {ECO:0000303|PubMed:29315936};
DE EC=4.2.1.174 {ECO:0000269|PubMed:29315936};
DE AltName: Full=Terpene synthase 1 {ECO:0000303|PubMed:29315936};
DE Short=VacTPS1 {ECO:0000303|PubMed:29315936};
DE Flags: Precursor;
GN Name=TPS1 {ECO:0000303|PubMed:29315936};
OS Vitex agnus-castus (Chaste tree).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Lamiales; Lamiaceae; Viticoideae; Vitex.
OX NCBI_TaxID=54477;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND
RP TISSUE SPECIFICITY.
RC TISSUE=Fruit, Leaf, and Trichome gland;
RX PubMed=29315936; DOI=10.1111/tpj.13822;
RA Heskes A.M., Sundram T.C.M., Boughton B.A., Jensen N.B., Hansen N.L.,
RA Crocoll C., Cozzi F., Rasmussen S., Hamberger B., Hamberger B., Staerk D.,
RA Moeller B.L., Pateraki I.;
RT "Biosynthesis of bioactive diterpenoids in the medicinal plant Vitex agnus-
RT castus.";
RL Plant J. 93:943-958(2018).
RN [2]
RP REVIEW ON MENSTRUAL CYCLE DISORDERS.
RX PubMed=12809367; DOI=10.1078/094471103322004866;
RA Wuttke W., Jarry H., Christoffel V., Spengler B., Seidlova-Wuttke D.;
RT "Chaste tree (Vitex agnus-castus)--pharmacology and clinical indications.";
RL Phytomedicine 10:348-357(2003).
RN [3]
RP PATHWAY, AND REVIEW.
RX PubMed=30468448; DOI=10.1039/c8np00077h;
RA Liu Y., Jing S.-X., Luo S.-H., Li S.-H.;
RT "Non-volatile natural products in plant glandular trichomes: chemistry,
RT biological activities and biosynthesis.";
RL Nat. Prod. Rep. 36:626-665(2019).
CC -!- FUNCTION: Involved in the biosynthesis of labdane-type diterpenoid
CC including cleroda-dienols, and peregrinol lactones and furan
CC derivatives, dopaminergic diterpenoids that can bind to dopamine
CC receptors in the human pituitary gland, have probably ability to lower
CC prolactin levels, and are used to treat menstrual cycle disorders (e.g.
CC premenstrual syndrome and mastodynia) (Probable). Terpene synthase that
CC produces peregrinol diphosphate from geranylgeranyl diphosphate (GGPP)
CC (PubMed:29315936). {ECO:0000269|PubMed:29315936,
CC ECO:0000305|PubMed:12809367, ECO:0000305|PubMed:29315936,
CC ECO:0000305|PubMed:30468448}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=peregrinol diphosphate = (2E,6E,10E)-geranylgeranyl
CC diphosphate + H2O; Xref=Rhea:RHEA:54652, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:58756, ChEBI:CHEBI:138232; EC=4.2.1.174;
CC Evidence={ECO:0000269|PubMed:29315936};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:54654;
CC Evidence={ECO:0000269|PubMed:29315936};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:Q38802};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000305|PubMed:29315936, ECO:0000305|PubMed:30468448}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in trichomes of leaves and fruits.
CC {ECO:0000269|PubMed:29315936}.
CC -!- DOMAIN: The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic
CC activity, presumably through binding to Mg(2+). {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR EMBL; MG696748; AUT77120.1; -; mRNA.
DR AlphaFoldDB; A0A2K9RFZ7; -.
DR SMR; A0A2K9RFZ7; -.
DR UniPathway; UPA00213; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0106238; F:peregrinol diphosphate synthase activity; IDA:UniProtKB.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Isomerase; Lyase; Magnesium; Metal-binding; Plastid;
KW Transit peptide.
FT TRANSIT 1..25
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 26..798
FT /note="Peregrinol diphosphate synthase TPS1, chloroplastic"
FT /id="PRO_0000449307"
FT MOTIF 377..380
FT /note="DXDD motif"
FT /evidence="ECO:0000305"
FT BINDING 243
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q38802"
FT BINDING 377
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:C7BKP9"
FT BINDING 379
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:C7BKP9"
FT BINDING 463
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q38802"
SQ SEQUENCE 798 AA; 92226 MW; 20083B50500EB797 CRC64;
MASLSTPNIN NTTFVNSKTQ LPAVKVHLQK CYVGPWLNRG SKHMFTNYQF GHRQISKVAK
YQASPDVVQV CDKVEHSTAQ SFELVDKKIE DNIRYVKELL NSIDDGHISV SAYDTAWFAL
IRDLDGRDCP QFPSTIEWIA DNQLADGSWG DEDFYSAYDR LINTLACVLA LRTWNVHPEK
SEKGISYIKE NLHELEDAEA ENMTCAFELL FPVLLKRAEN LGINEIPYDA PIIKEIYNIR
DTKLTRIPLE VLHERSTSIL YGMEGLENLD LDWQKLMKLQ TPEGSFLTSP AATAFAFMYT
KDENCLKYIK YILDKFNGAA VDVYPVDLFA RLWAVDRLQR LGISRFFESE IKDCLSYVHR
FWTEKGIFSG RHALFHDLDD TSMGFRLLRQ HGYDMDPNVF KHFQKDGRFH CLGGDMSDSL
TVTYNLYRAS QTQFPGEEIL EEARNFCYNF LQDRAARNQL VDKWVISKHL ADEMRTGLQL
PWYASLPRVE ARYYLQHYAG SGDVWLGKNF FRMEDISNDK YKEIAKLDFS RCQAQHQFEW
TYMQGWYESS NVQEFGISRK DLLVAYFLAA ATIFERERTK ERIVWAKSHI VSRMIKSFFT
NETTSLEEKV ALLTGFEDNI NGLHKITSAK REHEHVDILL ATLHQLLGEF DEYASHQLKN
AWRVWLTKLE QGEAGAEAEL LVTTLNICAG HDIAFKEDIL SQNEYKTLSN LTNKICQQLT
QIQNKKVMET NDSNSIQDKE IEHDMQALVK SVLEEAVGID RNIKQTFLSV AKTYYYGAYI
AAETIDVHIF KVLFEPVI
