ID   TPS2_ANTMA              Reviewed;         581 AA.
AC   Q84ND0;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Tricyclene synthase Oc15, chloroplastic;
DE            Short=AmOc15;
DE            EC=4.2.3.105;
DE   AltName: Full=Myrcene synthase Oc15;
DE            EC=4.2.3.15;
DE   AltName: Full=Terpenoid synthase Oc15;
DE   Flags: Precursor;
GN   Name=Oc15;
OS   Antirrhinum majus (Garden snapdragon).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Plantaginaceae; Antirrhineae; Antirrhinum.
OX   NCBI_TaxID=4151;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, DEVELOPMENTAL STAGE, CATALYTIC ACTIVITY, AND
RP   CIRCADIAN-REGULATION.
RC   TISSUE=Petal;
RX   PubMed=12724546; DOI=10.1105/tpc.011015;
RA   Dudareva N., Martin D., Kish C.M., Kolosova N., Gorenstein N., Faeldt J.,
RA   Miller B., Bohlmann J.;
RT   "(E)-beta-ocimene and myrcene synthase genes of floral scent biosynthesis
RT   in snapdragon: function and expression of three terpene synthase genes of a
RT   new terpene synthase subfamily.";
RL   Plant Cell 15:1227-1241(2003).
CC   -!- FUNCTION: Contributes to floral scent emission.
CC       {ECO:0000269|PubMed:12724546}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-geranyl diphosphate = diphosphate + tricyclene;
CC         Xref=Rhea:RHEA:32687, ChEBI:CHEBI:33019, ChEBI:CHEBI:58057,
CC         ChEBI:CHEBI:64266; EC=4.2.3.105;
CC         Evidence={ECO:0000269|PubMed:12724546};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-geranyl diphosphate = beta-myrcene + diphosphate;
CC         Xref=Rhea:RHEA:16965, ChEBI:CHEBI:17221, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58057; EC=4.2.3.15;
CC         Evidence={ECO:0000269|PubMed:12724546};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Accumulates in flowers; mostly expressed in both
CC       upper and lower petal lobes, and, to a lower extent, in tube and
CC       stamens. {ECO:0000269|PubMed:12724546}.
CC   -!- DEVELOPMENTAL STAGE: First observed in mature flower buds and
CC       accumulates transiently during 4 days after anthesis.
CC       {ECO:0000269|PubMed:12724546}.
CC   -!- INDUCTION: Circadian-regulation with highest levels in early afternoon
CC       and lowest levels during the night.
CC   -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC       the catalytic activity, presumably through binding to Mg(2+).
CC   -!- SIMILARITY: Belongs to the terpene synthase family. Tpsg subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AY195608; AAO41726.1; -; mRNA.
DR   AlphaFoldDB; Q84ND0; -.
DR   SMR; Q84ND0; -.
DR   UniPathway; UPA00213; -.
DR   GO; GO:0009570; C:chloroplast stroma; ISS:UniProtKB.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0050551; F:myrcene synthase activity; IDA:UniProtKB.
DR   GO; GO:0010333; F:terpene synthase activity; IDA:UniProtKB.
DR   GO; GO:0102701; F:tricyclene synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0007623; P:circadian rhythm; IEP:UniProtKB.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Glycoprotein; Lyase; Magnesium; Manganese; Metal-binding;
KW   Plastid; Transit peptide.
FT   TRANSIT         1..68
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           69..581
FT                   /note="Tricyclene synthase Oc15, chloroplastic"
FT                   /id="PRO_0000418162"
FT   MOTIF           338..342
FT                   /note="DDXXD motif"
FT   BINDING         338
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         338
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         342
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         342
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         482
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   BINDING         486
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   BINDING         490
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        27
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        206
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        319
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        384
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        465
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        509
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   581 AA;  66705 MW;  71A795B10A46359C CRC64;
     MAFCISYVGA LLPCSLSTRT KFAICHNTSK LHRAAYKTSR WNIPGDVGST PPPSKLHQAL
     CLNEHSLSCM AELPMDYEGK IKETRHLLHL KGENDPIESL IFVDATLRLG VNHHFQKEIE
     EILRKSYATM KSPIICEYHT LHEVSLFFRL MRQHGRYVSA DVFNNFKGES GRFKEELKRD
     TRGLVELYEA AQLSFEGERI LDEAENFSRQ ILHGNLAGME DNLRRSVGNK LRYPFHTSIA
     RFTGRNYDDD LGGMYEWGKT LRELALMDLQ VERSVYQEEL LQVSKWWNEL GLYKKLNLAR
     NRPFEFYTWS MVILADYINL SEQRVELTKS VAFIYLIDDI FDVYGTLDEL IIFTEAVNKW
     DYSATDTLPE NMKMCCMTLL DTINGTSQKI YEKHGYNPID SLKTTWKSLC SAFLVEAKWS
     ASGSLPSANE YLENEKVSSG VYVVLVHLFC LMGLGGTSRG SIELNDTQEL MSSIAIIFRL
     WNDLGSAKNE HQNGKDGSYL NCYKKEHINL TAAQAHEHAL ELVAIEWKRL NKESFNLNHD
     SVSSFKQAAL NLARMVPLMY SYDHNQRGPV LEEYVKFMLS D