TPS3_COFAR
ID TPS3_COFAR Reviewed; 596 AA.
AC R4YZC3;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2013, sequence version 1.
DT 03-AUG-2022, entry version 33.
DE RecName: Full=Linalool synthase TPS3, chloroplastic {ECO:0000303|PubMed:23398891};
DE EC=4.2.3.- {ECO:0000269|PubMed:23398891};
DE AltName: Full=Cis-ocimene synthase TPS2, chloroplastic {ECO:0000303|PubMed:23398891};
DE EC=4.2.3.- {ECO:0000269|PubMed:23398891};
DE AltName: Full=Myrcene synthase TPS3, chloroplastic {ECO:0000303|PubMed:23398891};
DE EC=4.2.3.15 {ECO:0000269|PubMed:23398891};
DE AltName: Full=Trans-ocimene synthase TPS2, chloroplastic {ECO:0000303|PubMed:23398891};
DE EC=4.2.3.106 {ECO:0000269|PubMed:23398891};
DE Flags: Precursor;
GN Name=TPS3 {ECO:0000303|PubMed:23398891};
GN ORFNames=LOC113713511 {ECO:0000312|RefSeq:XP_027093045.1},
GN LOC113713556 {ECO:0000312|RefSeq:XP_027093111.1};
OS Coffea arabica (Arabian coffee).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Gentianales; Rubiaceae; Ixoroideae; Gardenieae complex;
OC Bertiereae - Coffeeae clade; Coffeeae; Coffea.
OX NCBI_TaxID=13443;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Catuai Red; TISSUE=Flower, Fruit, and Seed;
RX PubMed=23398891; DOI=10.1016/j.phytochem.2013.01.005;
RA Del Terra L., Lonzarich V., Asquini E., Navarini L., Graziosi G.,
RA Suggi Liverani F., Pallavicini A.;
RT "Functional characterization of three Coffea arabica L. monoterpene
RT synthases: insights into the enzymatic machinery of coffee aroma.";
RL Phytochemistry 89:6-14(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Caturra red;
RA Zimin A.V., Yepes M., Maldonado C.E., Navarro L., Kovaka S., Pertea M.,
RA Gaitan A., Aldwinckle H.;
RT "The Coffea arabica cultivar Caturra genome provides a strong foundation
RT for breeding and functional genomics studies in coffee.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Monoterpene synthase (mono-TPS) involved in the biosynthesis
CC of monoterpenes natural products, constituent of coffee beverage aroma
CC (PubMed:23398891). Catalyzes the conversion of (2E)-geranyl diphosphate
CC (GPP) into linalool and beta-myrcene, and, as minor products, cis-
CC ocimene and trans-ocimene (PubMed:23398891). Not able to use
CC geranylgeranyl pyrophosphate (GGPP) and farnesyl pyrophosphate (FPP) as
CC substrates (PubMed:23398891). {ECO:0000269|PubMed:23398891}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = beta-myrcene + diphosphate;
CC Xref=Rhea:RHEA:16965, ChEBI:CHEBI:17221, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58057; EC=4.2.3.15;
CC Evidence={ECO:0000269|PubMed:23398891};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16966;
CC Evidence={ECO:0000269|PubMed:23398891};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate + H2O = diphosphate + linalool;
CC Xref=Rhea:RHEA:68708, ChEBI:CHEBI:15377, ChEBI:CHEBI:17580,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58057;
CC Evidence={ECO:0000269|PubMed:23398891};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68709;
CC Evidence={ECO:0000269|PubMed:23398891};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = (Z)-beta-ocimene + diphosphate;
CC Xref=Rhea:RHEA:68824, ChEBI:CHEBI:33019, ChEBI:CHEBI:58057,
CC ChEBI:CHEBI:87574; Evidence={ECO:0000269|PubMed:23398891};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68825;
CC Evidence={ECO:0000269|PubMed:23398891};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = (E)-beta-ocimene + diphosphate;
CC Xref=Rhea:RHEA:32691, ChEBI:CHEBI:33019, ChEBI:CHEBI:58057,
CC ChEBI:CHEBI:64280; EC=4.2.3.106;
CC Evidence={ECO:0000269|PubMed:23398891};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32692;
CC Evidence={ECO:0000269|PubMed:23398891};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:A0A1C9J6A7};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000250|UniProtKB:A0A1C9J6A7};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit.
CC {ECO:0000250|UniProtKB:A0A1C9J6A7};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000269|PubMed:23398891}.
CC -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q6JD73}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers and fruits.
CC {ECO:0000269|PubMed:23398891}.
CC -!- DEVELOPMENTAL STAGE: Observed at early stages of flowers and fruits
CC development (PubMed:23398891). Expressed in flowers and drupes at 10
CC weeks after pollination, and, at low levels, in fruits at 15 weeks of
CC ripening (PubMed:23398891). {ECO:0000269|PubMed:23398891}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsb subfamily.
CC {ECO:0000305}.
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DR EMBL; HE985294; CCM43929.1; -; mRNA.
DR RefSeq; XP_027093045.1; XM_027237244.1.
DR RefSeq; XP_027093111.1; XM_027237310.1.
DR UniPathway; UPA00213; -.
DR Proteomes; UP000515148; Chromosome 10c.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0050551; F:myrcene synthase activity; IDA:UniProtKB.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR GO; GO:0016098; P:monoterpenoid metabolic process; IDA:UniProtKB.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Metal-binding; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..39
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 40..596
FT /note="Linalool synthase TPS3, chloroplastic"
FT /id="PRO_0000455260"
FT MOTIF 345..349
FT /note="DDXXD motif"
FT /evidence="ECO:0000305"
FT BINDING 345
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 345
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 349
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 349
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 489
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 493
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
SQ SEQUENCE 596 AA; 68839 MW; C8A170F9DE2AD5A8 CRC64;
MISSLNPLFT THRSGVIAQQ FFASSAAASI NSVSSLKIAA CSKTKLVDQS PLRQSGNHQL
LSSDFNHLQS LKNDYAEEKY KSRCEVLKEQ VKMMLDQEMD VVNQLELIDD LQRLGLSYHF
GDEITSVLSG IYNRKSMNKM RNQWGLYATC LEFRLLRQHG FDVSQEIFDC FKDEKGDFRP
SLCEDSKGML YLYEASYLES ENEESNLEMA RRFAAKTLKK NLDEKRVDQD LVALVQHALE
LPLHWRMMRL EARWFIDIYE ERSNRNPILL ELAKLDFNIV QAAHQNDLTY TLRWWRSTCL
AEKLTFARDM MVENFFWTVG IISDPQRGNG RRILTKVVAL ITAIDDIYDC YGTLDELEVF
TTAVERWDVN SIDQLPDCMK ICFLALYNFV NEMAYDALKE QGVNIIPYLR KSWADLCKAY
LQEAKWFFSG EVPTLQQYLN NAWISISAPA FLVHAYFCVD YPINKDHLQY LDNYHKIIRC
SAMILRLTND LGTSPESEVL NVGDVPKSIR CYMKETGACE EKAREHLRFL ITEAWKQMEE
AQTLDSPFSS TFNGIAVNLA RMGLCMYQHG DGHGHQNSEP RDRILSLLFE PICCLA
