TPS3_MAIZE
ID TPS3_MAIZE Reviewed; 574 AA.
AC Q29VN1; A0A1D6GYX5;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Probable terpene synthase 3, chloroplastic {ECO:0000305};
DE EC=4.2.3.- {ECO:0000250|UniProtKB:Q29VN2};
DE Flags: Precursor;
GN Name=TPS3 {ECO:0000303|PubMed:27662898, ECO:0000312|EMBL:AAX99150.1};
GN ORFNames=ZEAMMB73_Zm00001d015054 {ECO:0000312|EMBL:AQK67959.1};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, AND PHYLOGENETIC ANALYSIS.
RC STRAIN=cv. B73;
RX PubMed=27662898; DOI=10.1105/tpc.15.00919;
RA Richter A., Schaff C., Zhang Z., Lipka A.E., Tian F., Koellner T.G.,
RA Schnee C., Preiss S., Irmisch S., Jander G., Boland W., Gershenzon J.,
RA Buckler E.S., Degenhardt J.;
RT "Characterization of biosynthetic pathways for the production of the
RT volatile homoterpenes DMNT and TMTT in Zea mays.";
RL Plant Cell 28:2651-2665(2016).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73;
RX PubMed=19965430; DOI=10.1126/science.1178534;
RA Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT "The B73 maize genome: complexity, diversity, and dynamics.";
RL Science 326:1112-1115(2009).
RN [3]
RP INDUCTION.
RX PubMed=26378100; DOI=10.1104/pp.15.01039;
RA Tzin V., Fernandez-Pozo N., Richter A., Schmelz E.A., Schoettner M.,
RA Schaefer M., Ahern K.R., Meihls L.N., Kaur H., Huffaker A., Mori N.,
RA Degenhardt J., Mueller L.A., Jander G.;
RT "Dynamic maize responses to aphid feeding are revealed by a time series of
RT transcriptomic and metabolomic assays.";
RL Plant Physiol. 169:1727-1743(2015).
RN [4]
RP REVIEW.
RX PubMed=30187155; DOI=10.1007/s00425-018-2999-2;
RA Block A.K., Vaughan M.M., Schmelz E.A., Christensen S.A.;
RT "Biosynthesis and function of terpenoid defense compounds in maize (Zea
RT mays).";
RL Planta 249:21-30(2019).
CC -!- FUNCTION: Probable terpene synthase. {ECO:0000250|UniProtKB:Q29VN2}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:O81192};
CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250|UniProtKB:O81192};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000305|PubMed:30187155}.
CC -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q6JD73}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000250|UniProtKB:Q29VN2}.
CC -!- INDUCTION: By a musk indanone elicitor that mimics herbivory
CC (PubMed:27662898). By corn leaf aphid feeding (PubMed:26378100).
CC {ECO:0000269|PubMed:26378100, ECO:0000269|PubMed:27662898}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000250|UniProtKB:A0A1C9J6A7}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000255,
CC ECO:0000305}.
CC -!- CAUTION: Does not produce any detectable product when tested in vitro.
CC {ECO:0000305|PubMed:27662898}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY928082; AAX99150.1; -; mRNA.
DR EMBL; CM000781; AQK67959.1; -; Genomic_DNA.
DR RefSeq; NP_001105855.1; NM_001112385.1.
DR AlphaFoldDB; Q29VN1; -.
DR SMR; Q29VN1; -.
DR STRING; 4577.GRMZM2G064406_P01; -.
DR PaxDb; Q29VN1; -.
DR PRIDE; Q29VN1; -.
DR GeneID; 732759; -.
DR KEGG; zma:732759; -.
DR MaizeGDB; 9022637; -.
DR eggNOG; ENOG502QTGK; Eukaryota.
DR HOGENOM; CLU_003125_7_1_1; -.
DR OMA; CLFNEAI; -.
DR OrthoDB; 449049at2759; -.
DR UniPathway; UPA00213; -.
DR Proteomes; UP000007305; Chromosome 5.
DR GO; GO:0009507; C:chloroplast; ISS:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0031347; P:regulation of defense response; IEP:UniProtKB.
DR GO; GO:0009611; P:response to wounding; IEP:UniProtKB.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Lyase; Magnesium; Manganese; Metal-binding; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..55
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 56..574
FT /note="Probable terpene synthase 3, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000440868"
FT MOTIF 329..333
FT /note="DDXXD motif"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 301
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:O81192"
FT BINDING 329
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:O81192"
FT BINDING 329
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:O81192"
FT BINDING 329
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 333
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:O81192"
FT BINDING 333
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:O81192"
FT BINDING 333
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 473
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:O81192"
FT BINDING 476
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:O81192"
FT BINDING 480
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 484
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:O81192"
SQ SEQUENCE 574 AA; 64780 MW; 192520E70E55CF11 CRC64;
MYSLPGATMS AAPARVISSS SSSSFVEPLL LAAASSAAAN SHHQVRQRGH LVRTLAASSS
SNTLLRSDFD LQEGLTTDVK RMLRQRQKKS GGGREMLVTI DNLKRLCIDH FFEEEIEGAM
ATGACTRLLH SDDLFDATLA FRLLREAGHD VSAKEDVLRR FIDGVSGDFK LSLNNDVRGL
LGLHDMSHLD VGGEEAALLH RAKEFSSSHL ASAVRYQDNP SLAEYVRQSL DHPYHLSLTQ
YKARHHLRYL QSLPSSCRDA AVERLAVAEF QLNKSLHQRE MREIKRWWMD LGLAEEIPVV
RDQVMKWYMW SMAALQGSSF SRYRVYVVDD IFDLVGTLEE LSAFTEAVKM WDTAAADSLP
SCMRSCYKAL HTVTNEIAEI AHKEHGSNPI NRLRKAWVVL FDGFMVEARW LATDQVPTAE
DYLRNGVVTS GVPLTFLHIF SMLGYDDPST EEEEEAIIDH MPSIISCPAK ILRLWDDMGS
AEDEAQEGFD GSYRDFYLME NPSRSPGEAE AHMRGLIARE WVELNRECFC RRTFPSDIAQ
VCLNTARMVS VMYSYNKEQR LLVLEDYAAM MLVL
