TPS4_ARATH
ID TPS4_ARATH Reviewed; 795 AA.
AC Q9T079;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 4;
DE EC=2.4.1.15;
DE AltName: Full=Trehalose-6-phosphate synthase 4;
DE Short=AtTPS4;
GN Name=TPS4; OrderedLocusNames=At4g27550; ORFNames=T29A15.40;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11701378; DOI=10.1016/s1360-1385(01)02125-2;
RA Leyman B., Van Dijck P., Thevelein J.M.;
RT "An unexpected plethora of trehalose biosynthesis genes in Arabidopsis
RT thaliana.";
RL Trends Plant Sci. 6:510-513(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-
CC trehalose 6-phosphate + H(+) + UDP; Xref=Rhea:RHEA:18889,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58429,
CC ChEBI:CHEBI:58885, ChEBI:CHEBI:61548; EC=2.4.1.15;
CC -!- SIMILARITY: In the N-terminal section; belongs to the
CC glycosyltransferase 20 family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the trehalose
CC phosphatase family. {ECO:0000305}.
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DR EMBL; AL035602; CAB38267.1; -; Genomic_DNA.
DR EMBL; AL161571; CAB81405.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85356.1; -; Genomic_DNA.
DR PIR; T05860; T05860.
DR RefSeq; NP_194485.1; NM_118890.2.
DR AlphaFoldDB; Q9T079; -.
DR SMR; Q9T079; -.
DR STRING; 3702.AT4G27550.1; -.
DR CAZy; GT20; Glycosyltransferase Family 20.
DR PaxDb; Q9T079; -.
DR PRIDE; Q9T079; -.
DR ProteomicsDB; 232501; -.
DR EnsemblPlants; AT4G27550.1; AT4G27550.1; AT4G27550.
DR GeneID; 828864; -.
DR Gramene; AT4G27550.1; AT4G27550.1; AT4G27550.
DR KEGG; ath:AT4G27550; -.
DR Araport; AT4G27550; -.
DR TAIR; locus:2137712; AT4G27550.
DR eggNOG; KOG1050; Eukaryota.
DR HOGENOM; CLU_002351_3_3_1; -.
DR InParanoid; Q9T079; -.
DR OrthoDB; 772297at2759; -.
DR PhylomeDB; Q9T079; -.
DR PRO; PR:Q9T079; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9T079; baseline and differential.
DR Genevisible; Q9T079; AT.
DR GO; GO:0003825; F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity; ISS:TAIR.
DR GO; GO:0004805; F:trehalose-phosphatase activity; IEA:UniProt.
DR GO; GO:0005992; P:trehalose biosynthetic process; ISS:TAIR.
DR GO; GO:0070413; P:trehalose metabolism in response to stress; IBA:GO_Central.
DR CDD; cd03788; GT20_TPS; 1.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR001830; Glyco_trans_20.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR003337; Trehalose_PPase.
DR PANTHER; PTHR10788; PTHR10788; 1.
DR Pfam; PF00982; Glyco_transf_20; 1.
DR Pfam; PF02358; Trehalose_PPase; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..795
FT /note="Probable alpha,alpha-trehalose-phosphate synthase
FT [UDP-forming] 4"
FT /id="PRO_0000324825"
FT REGION 4..469
FT /note="Glycosyltransferase"
SQ SEQUENCE 795 AA; 89474 MW; 4B9191CB2CE56126 CRC64;
MARPRLLVVS MSLPVTAKRT GEESWSFTMS PGGLVSALLG LKEFETKWIG WPGVDVHDAI
GKKTLSITLA EKGCIPVFLE EVCDQYYNGY CNNILWPIFH YLGTPPEYRN DATITYQSQY
EAYKKANQIF FDVVKEHYEE GDVVWCHDYH VMLLPQYLKE YNSKMKVGWF LHTPFPSSEM
YKTLPSRSDL LRSVLTADLV GFHTYDFARH FLNACMCILG VEATSEGIVD QGKVTRVAVF
PIGIEPERFI NTSELSEVVQ YMKKFKNDFG GRKLILGVDR LDTIKGIPQK YQAFEKFLEE
NAEWRGKVML LQIAVPTRNG IGEYQKIKDQ CHYHVGRING RFGSISSVPI IHLDCSIDFN
QLCALYAITD VLLVTSLRDG MNLVSSEFIA CQKAEKGVLI LSEFAGAGQS LGAGAILVNP
WNIKEVSSAI GEALNMSHEE KERKHKINFQ YVKTHSTQQW ADDFMKLTLT NILCSKLIEI
TTSAELGAGL AATLELPEHD VIQQYSKSNN RLLILGFYGT LTQPMKNQER RGDGMNLELH
PQLKERLKEL CSDPKTTVVV LSRSEKCILD KNFGEYNMWL AAENGMFLRH TSGEWVTRIP
EHMNLEWIDG VKHVFKYFTE RTPGSYLETS EASLVWNYEN ADAEFGRAQA RDMLQHLWAG
PISNASVDVV RGGQSVEVHA VGVTKGSAME RILGEIVHNK SMATPIDYVL CIGCFLGKDE
DVYTFFEPEL TKKAKSLSSS GSDSPKKVSS TIVDLKGENY FSVAIGQTHT KARYFLDSSD
DVVKLIGKLC THNNA
