TPS5_RICCO
ID TPS5_RICCO Reviewed; 552 AA.
AC B9SBU9; G5CT98;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2013, sequence version 2.
DT 03-AUG-2022, entry version 50.
DE RecName: Full=Terpene synthase 5;
DE Short=RcSeTPS5;
DE EC=4.2.3.-;
GN Name=TPS5; ORFNames=RCOM_1044790;
OS Ricinus communis (Castor bean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Euphorbiaceae; Acalyphoideae; Acalypheae;
OC Ricinus.
OX NCBI_TaxID=3988;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=22459969; DOI=10.1016/j.phytochem.2012.02.022;
RA Xie X., Kirby J., Keasling J.D.;
RT "Functional characterization of four sesquiterpene synthases from Ricinus
RT communis (castor bean).";
RL Phytochemistry 78:20-28(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Hale;
RX PubMed=20729833; DOI=10.1038/nbt.1674;
RA Chan A.P., Crabtree J., Zhao Q., Lorenzi H., Orvis J., Puiu D.,
RA Melake-Berhan A., Jones K.M., Redman J., Chen G., Cahoon E.B., Gedil M.,
RA Stanke M., Haas B.J., Wortman J.R., Fraser-Liggett C.M., Ravel J.,
RA Rabinowicz P.D.;
RT "Draft genome sequence of the oilseed species Ricinus communis.";
RL Nat. Biotechnol. 28:951-956(2010).
CC -!- FUNCTION: Catalyzes the cyclization of farnesyl diphosphate to multiple
CC sesquiterpenes, such as olefins and sesquiterpene alcohols.
CC {ECO:0000269|PubMed:22459969}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250};
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EEF38927.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; JN315865; AEQ27767.1; -; mRNA.
DR EMBL; EQ973917; EEF38927.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; NP_001310688.1; NM_001323759.1.
DR RefSeq; XP_015577437.1; XM_015721951.1.
DR AlphaFoldDB; B9SBU9; -.
DR SMR; B9SBU9; -.
DR PRIDE; B9SBU9; -.
DR GeneID; 8282476; -.
DR KEGG; rcu:8282476; -.
DR InParanoid; B9SBU9; -.
DR OrthoDB; 360509at2759; -.
DR Proteomes; UP000008311; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010334; F:sesquiterpene synthase activity; IDA:UniProtKB.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR GO; GO:1900674; P:olefin biosynthetic process; IDA:UniProtKB.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 2: Evidence at transcript level;
KW Lyase; Magnesium; Metal-binding; Reference proteome.
FT CHAIN 1..552
FT /note="Terpene synthase 5"
FT /id="PRO_0000422203"
FT MOTIF 307..311
FT /note="DDXXD motif"
FT BINDING 307
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 307
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 311
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 311
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 457
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT CONFLICT 335
FT /note="I -> T (in Ref. 1; AEQ27767)"
FT /evidence="ECO:0000305"
FT CONFLICT 377
FT /note="L -> V (in Ref. 1; AEQ27767)"
FT /evidence="ECO:0000305"
FT CONFLICT 428
FT /note="F -> I (in Ref. 1; AEQ27767)"
FT /evidence="ECO:0000305"
FT CONFLICT 505
FT /note="T -> I (in Ref. 1; AEQ27767)"
FT /evidence="ECO:0000305"
FT CONFLICT 518
FT /note="V -> A (in Ref. 1; AEQ27767)"
FT /evidence="ECO:0000305"
FT CONFLICT 524
FT /note="T -> S (in Ref. 1; AEQ27767)"
FT /evidence="ECO:0000305"
FT CONFLICT 541
FT /note="F -> S (in Ref. 1; AEQ27767)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 552 AA; 64624 MW; 726DD4FA149F0AC5 CRC64;
MATEGLLSAE TDQDVARFLA NFPPTEWGYS FASLLPQDSE FESHTKELDL VKEKVKDMLM
QSRKELTENI EFVNCLCRLG VSYHFESEII EQLSHIFISL PKILEENDYS LYILTLLFRV
LRQHGYKMPC DVFNKFKDSN GEFKKCMTAD VRGLLSLYEA TFLSVHGEDI LDEALAFTRQ
HLETLAEKSS PHLARHIRNA LHLPFHHAPE RLEILQYICF YEGEKSMNET LLKFAKLDFN
RLQLLYRKEL GLLSRWWKDI NLTEKLPYTR DRIVEAYAWA AGIIIDPQFS RARLQFAKHL
ILISVMDDTY DSYGTFDELK HFTAALQRFT FEPTIELPEY MKFLYNILWN FFQETEKDET
QGCACKTSFA REMLKELARS YFAEAEWYND GVLPTFDEFM QFGLVSSTFD YHASAFFLGV
EDLGMKEFIW LRDNPTIAKT AKLFGRLFND IAIREDEQKK GDYPSAIKCY MNDHDVSLEK
AKEDILKMLE DGWKDMNEEL MKPTTVPKIL TKFSINFVRM SDFTYRGGID KYTCGTELKE
FVKKLTIFPL QI
