BTUF_SALTY
ID BTUF_SALTY Reviewed; 266 AA.
AC Q8ZRP7; Q9ZFP9;
DT 05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 05-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Vitamin B12-binding protein;
DE Flags: Precursor;
GN Name=btuF; OrderedLocusNames=STM0206;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=10464235; DOI=10.1128/jb.181.17.5539-5541.1999;
RA Van Bibber M., Bradbeer C., Clark N., Roth J.R.;
RT "A new class of cobalamin transport mutants (btuF) provides genetic
RT evidence for a periplasmic binding protein in Salmonella typhimurium.";
RL J. Bacteriol. 181:5539-5541(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Part of the ABC transporter complex BtuCDF involved in
CC vitamin B12 import. Binds vitamin B12 and delivers it to the
CC periplasmic surface of BtuC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (BtuD),
CC two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the BtuF family. {ECO:0000305}.
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DR EMBL; AF096877; AAC98382.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL19170.1; -; Genomic_DNA.
DR RefSeq; NP_459211.1; NC_003197.2.
DR RefSeq; WP_001118843.1; NC_003197.2.
DR AlphaFoldDB; Q8ZRP7; -.
DR SMR; Q8ZRP7; -.
DR STRING; 99287.STM0206; -.
DR TCDB; 3.A.1.13.1; the atp-binding cassette (abc) superfamily.
DR PaxDb; Q8ZRP7; -.
DR EnsemblBacteria; AAL19170; AAL19170; STM0206.
DR GeneID; 1251724; -.
DR KEGG; stm:STM0206; -.
DR PATRIC; fig|99287.12.peg.219; -.
DR HOGENOM; CLU_038034_2_5_6; -.
DR OMA; WQGINLE; -.
DR PhylomeDB; Q8ZRP7; -.
DR BioCyc; SENT99287:STM0206-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0015889; P:cobalamin transport; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01000; BtuF; 1.
DR InterPro; IPR002491; ABC_transptr_periplasmic_BD.
DR InterPro; IPR023544; ABC_transptr_vit_B12-bd.
DR Pfam; PF01497; Peripla_BP_2; 1.
DR PROSITE; PS50983; FE_B12_PBP; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Periplasm; Reference proteome; Signal; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..266
FT /note="Vitamin B12-binding protein"
FT /id="PRO_0000003506"
FT DOMAIN 25..266
FT /note="Fe/B12 periplasmic-binding"
FT BINDING 50
FT /ligand="cyanocob(III)alamin"
FT /ligand_id="ChEBI:CHEBI:17439"
FT /evidence="ECO:0000250"
FT BINDING 242..246
FT /ligand="cyanocob(III)alamin"
FT /ligand_id="ChEBI:CHEBI:17439"
FT /evidence="ECO:0000250"
FT SITE 72
FT /note="Important for BtuC binding"
FT /evidence="ECO:0000250"
FT SITE 202
FT /note="Important for BtuC binding"
FT /evidence="ECO:0000250"
FT DISULFID 183..259
FT /evidence="ECO:0000250"
FT CONFLICT 149
FT /note="E -> Q (in Ref. 1; AAC98382)"
FT /evidence="ECO:0000305"
FT CONFLICT 154
FT /note="A -> P (in Ref. 1; AAC98382)"
FT /evidence="ECO:0000305"
FT CONFLICT 157
FT /note="R -> H (in Ref. 1; AAC98382)"
FT /evidence="ECO:0000305"
FT CONFLICT 170
FT /note="S -> N (in Ref. 1; AAC98382)"
FT /evidence="ECO:0000305"
FT CONFLICT 181
FT /note="T -> A (in Ref. 1; AAC98382)"
FT /evidence="ECO:0000305"
FT CONFLICT 185
FT /note="G -> W (in Ref. 1; AAC98382)"
FT /evidence="ECO:0000305"
FT CONFLICT 198..200
FT /note="QVS -> HVI (in Ref. 1; AAC98382)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="A -> G (in Ref. 1; AAC98382)"
FT /evidence="ECO:0000305"
FT CONFLICT 210
FT /note="Q -> H (in Ref. 1; AAC98382)"
FT /evidence="ECO:0000305"
FT CONFLICT 218..224
FT /note="AGEILKI -> TVGNQRY (in Ref. 1; AAC98382)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 266 AA; 29284 MW; E08D0EFA813E2729 CRC64;
MAKQMFRALV ALLLTLPVWL YAAPRVITLS PANTELAFAA GITPVGVSSY SDYPPEAQKI
EQVSTWQGMN LERIVALKPD LVVAWRGGNA ERQVNQLTSL GIKVMWVDAV TIEQIADTLR
QLAAWSPQPE KAQQAAQTLL NEYAALNAEY AGKAKKRVFL QFGMNPLFTS GKGSIQHQVL
TTCGGENVFA DSRVPWPQVS REQVLARHPQ AIIVAGKAGE ILKIEQYWGN LLKIPVIPLN
SDWFERASPR IILAAKQLCN ALSQVN
