TPS7_HEDCO
ID TPS7_HEDCO Reviewed; 593 AA.
AC W6HUT3;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 29.
DE RecName: Full=Monoterpene synthase 7, chloroplastic {ECO:0000303|PubMed:25056927};
DE Short=HcTPS7 {ECO:0000303|PubMed:25056927};
DE AltName: Full=Alpha-pinene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.- {ECO:0000269|PubMed:25056927};
DE AltName: Full=Alpha-terpinene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.115 {ECO:0000269|PubMed:25056927};
DE AltName: Full=Beta-phellandrene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.- {ECO:0000269|PubMed:25056927};
DE AltName: Full=Beta-pinene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.- {ECO:0000269|PubMed:25056927};
DE AltName: Full=Gamma-terpinene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.114 {ECO:0000269|PubMed:25056927};
DE AltName: Full=Myrcene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.15 {ECO:0000269|PubMed:25056927};
DE AltName: Full=Sabinene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.- {ECO:0000269|PubMed:25056927};
DE AltName: Full=Terpinolene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.113 {ECO:0000269|PubMed:25056927};
DE Flags: Precursor;
GN Name=TPS7 {ECO:0000303|PubMed:25056927};
OS Hedychium coronarium (White butterfly ginger-lily).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Zingiberales; Zingiberaceae;
OC Hedychium.
OX NCBI_TaxID=71610;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP BIOPHYSICOCHEMICAL PROPERTIES, AND COFACTOR.
RX PubMed=25056927; DOI=10.1007/s00425-014-2127-x;
RA Yue Y., Yu R., Fan Y.;
RT "Characterization of two monoterpene synthases involved in floral scent
RT formation in Hedychium coronarium.";
RL Planta 240:745-762(2014).
CC -!- FUNCTION: Monoterpene synthase involved in the biosynthesis of volatile
CC compounds present in floral scent (PubMed:25056927). Mediates the
CC conversion of (2E)-geranyl diphosphate (GPP) into sabinene and sub-
CC products such as alpha-thujene, alpha-pinene, beta-pinene, myrcene,
CC alpha-phellandrene, alpha-terpinene, beta-phellandrene, gamma-terpinene
CC and terpinolene (PubMed:25056927). Unable to use farnesyl diphosphate
CC (FPP) as substrate (PubMed:25056927). {ECO:0000269|PubMed:25056927}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = diphosphate + sabinene;
CC Xref=Rhea:RHEA:68636, ChEBI:CHEBI:33019, ChEBI:CHEBI:50027,
CC ChEBI:CHEBI:58057; Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68637;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = diphosphate + terpinolene;
CC Xref=Rhea:RHEA:25500, ChEBI:CHEBI:9457, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58057; EC=4.2.3.113;
CC Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25501;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = alpha-pinene + diphosphate;
CC Xref=Rhea:RHEA:25662, ChEBI:CHEBI:33019, ChEBI:CHEBI:36740,
CC ChEBI:CHEBI:58057; Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25663;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = beta-pinene + diphosphate;
CC Xref=Rhea:RHEA:25666, ChEBI:CHEBI:33019, ChEBI:CHEBI:50025,
CC ChEBI:CHEBI:58057; Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25667;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = beta-myrcene + diphosphate;
CC Xref=Rhea:RHEA:16965, ChEBI:CHEBI:17221, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58057; EC=4.2.3.15;
CC Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16966;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = alpha-terpinene + diphosphate;
CC Xref=Rhea:RHEA:32563, ChEBI:CHEBI:10334, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58057; EC=4.2.3.115;
CC Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32564;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = beta-phellandrene + diphosphate;
CC Xref=Rhea:RHEA:25504, ChEBI:CHEBI:33019, ChEBI:CHEBI:48741,
CC ChEBI:CHEBI:58057; Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25505;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = diphosphate + gamma-terpinene;
CC Xref=Rhea:RHEA:32559, ChEBI:CHEBI:10577, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58057; EC=4.2.3.114;
CC Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32560;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:25056927};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000269|PubMed:25056927};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit.
CC {ECO:0000250|UniProtKB:A0A1C9J6A7};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=26.49 uM for (2E)-geranyl diphosphate
CC {ECO:0000269|PubMed:25056927};
CC Vmax=928.92 pmol/sec/mg enzyme with (2E)-geranyl diphosphate as
CC substrate {ECO:0000269|PubMed:25056927};
CC Note=kcat is 0.057 sec(-1) with (2E)-geranyl diphosphate as
CC substrate. {ECO:0000269|PubMed:25056927};
CC pH dependence:
CC Optimum pH is 7.5 (PubMed:25056927). Active at pH between 6.0 and 9.0
CC (PubMed:25056927). {ECO:0000269|PubMed:25056927};
CC Temperature dependence:
CC Optimum temperature is 30 degrees Celsius.
CC {ECO:0000269|PubMed:25056927};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000269|PubMed:25056927}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:25056927}.
CC -!- TISSUE SPECIFICITY: Highly expressed in flowers, petals and sepals, but
CC almost undetectable in vegetative organs.
CC {ECO:0000269|PubMed:25056927}.
CC -!- DEVELOPMENTAL STAGE: During flower development, first observed in
CC petals after about 40 hours, with a sharp increase between 32 and 48
CC hours, and a decrease during flower senescence.
CC {ECO:0000269|PubMed:25056927}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000250|UniProtKB:Q40577}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily.
CC {ECO:0000305}.
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DR EMBL; KF765488; AHJ57305.1; -; mRNA.
DR SMR; W6HUT3; -.
DR UniPathway; UPA00213; -.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0102903; F:gamma-terpinene synthase activity; IDA:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0050551; F:myrcene synthase activity; IDA:UniProtKB.
DR GO; GO:0050550; F:pinene synthase activity; IDA:UniProtKB.
DR GO; GO:0080015; F:sabinene synthase activity; IDA:UniProtKB.
DR GO; GO:0010333; F:terpene synthase activity; IDA:UniProtKB.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR GO; GO:0010597; P:green leaf volatile biosynthetic process; IDA:UniProtKB.
DR GO; GO:0016099; P:monoterpenoid biosynthetic process; IDA:UniProtKB.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Metal-binding; Plastid; Transit peptide.
FT TRANSIT 1..39
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 40..593
FT /note="Monoterpene synthase 7, chloroplastic"
FT /id="PRO_0000454954"
FT MOTIF 348..352
FT /note="DDXXD motif"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 348
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 348
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 352
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 352
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 491
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 499
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
SQ SEQUENCE 593 AA; 68541 MW; D4C65F6F448E4A7A CRC64;
MSVSLSFAAS ATFGFRGGLG GFSRPAAAIK QWRCLPRIQC HSAEQSQSPL RRSGNYQPSI
WTHDRIQSLT LSHTADEDDH GERIKLLKCQ TNKLMEEKKG EVGEQLQLID HLQQLGVAYH
FKDEIKDTLR GFYASFEDIS LQFKDNLHAS ALLFRLLREN GFSVSEDIFK KFKDDQKGQF
EDRLQSQAEG LLSLYEASYL EKDGEELLHE AREFTTKHLK NLLEEEGSLK PGLIREQVAY
ALELPLNRRF QRLHTKWFIG AWQRDPTMDP ALLLLAKLDF NALQNMYKRE LNEVSRWWTD
LGLPQKLPFF RDRLTENYLW AVVFAFEPDS WAFREMDTKT NCFITMIDDV YDVYGTLDEL
ELFTDIMERW DVNAIDKLPE YMKICFLAVF NTVNDAGYEV MRDKGVNIIP YLKRAWAELC
KMYMREARWY HTGYTPTLDE YLDGAWISIS GALILSTAYC MGKDLTKEDL DKFSTYPSIV
QPSCMLLRLH DDFGTSTEEL ARGDVQKAVQ CCMHERKVPE AVAREHIKQV MEAKWRVLNG
NRVAASSFEE YFQNVAINLP RAAQFFYGKG DGYANADGET QKQVMSLLIE PVQ
