TPS8_HEDCO
ID TPS8_HEDCO Reviewed; 591 AA.
AC U5PZT6;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 22-JAN-2014, sequence version 1.
DT 03-AUG-2022, entry version 29.
DE RecName: Full=Monoterpene synthase 8, chloroplastic {ECO:0000303|PubMed:25056927};
DE Short=HcTPS8 {ECO:0000303|PubMed:25056927};
DE AltName: Full=Alpha-bergamotene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.81 {ECO:0000269|PubMed:25056927};
DE AltName: Full=Alpha-curcumene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.- {ECO:0000269|PubMed:25056927};
DE AltName: Full=Beta-bisabolene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.55 {ECO:0000269|PubMed:25056927};
DE AltName: Full=Beta-farnesene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.47 {ECO:0000269|PubMed:25056927};
DE AltName: Full=Beta-sesquiphellandrene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.123 {ECO:0000269|PubMed:25056927};
DE AltName: Full=Cis-alpha-bisabolene synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.38 {ECO:0000269|PubMed:25056927};
DE AltName: Full=Linalool synthase {ECO:0000303|PubMed:25056927};
DE EC=4.2.3.25 {ECO:0000269|PubMed:25056927};
DE EC=4.2.3.26 {ECO:0000269|PubMed:25056927};
DE Flags: Precursor;
GN Name=TPS8 {ECO:0000303|PubMed:25056927};
OS Hedychium coronarium (White butterfly ginger-lily).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Zingiberales; Zingiberaceae;
OC Hedychium.
OX NCBI_TaxID=71610;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP BIOPHYSICOCHEMICAL PROPERTIES, AND COFACTOR.
RX PubMed=25056927; DOI=10.1007/s00425-014-2127-x;
RA Yue Y., Yu R., Fan Y.;
RT "Characterization of two monoterpene synthases involved in floral scent
RT formation in Hedychium coronarium.";
RL Planta 240:745-762(2014).
CC -!- FUNCTION: Sesquiterpene and monoterpene synthase involved in the
CC biosynthesis of volatile compounds present in floral scent
CC (PubMed:25056927). Mediates the conversion of (2E)-geranyl diphosphate
CC (GPP) into linalool, with trace levels of myrcene, limonene and (Z)-
CC beta-ocimene (PubMed:25056927). Acts also as a sesquiterpene synthase
CC by catalyzing the conversion of farnesyl diphosphate (FPP) to alpha-
CC bergamotene and beta-bisabolene and to minor products including alpha-
CC curcumene, cis-alpha-bisabolene, beta-farnesene and beta-
CC sesquiphellandrene, as well as seven other unidentified sesquiterpenes
CC (PubMed:25056927). {ECO:0000269|PubMed:25056927}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate + H2O = (R)-linalool + diphosphate;
CC Xref=Rhea:RHEA:15809, ChEBI:CHEBI:28, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58057; EC=4.2.3.26;
CC Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15810;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate + H2O = (S)-linalool + diphosphate;
CC Xref=Rhea:RHEA:24116, ChEBI:CHEBI:98, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58057; EC=4.2.3.25;
CC Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:24117;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = (S)-beta-bisabolene +
CC diphosphate; Xref=Rhea:RHEA:28266, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:49263, ChEBI:CHEBI:175763; EC=4.2.3.55;
CC Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28267;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = (E,R)-alpha-bisabolene +
CC diphosphate; Xref=Rhea:RHEA:25436, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:49243, ChEBI:CHEBI:175763; EC=4.2.3.38;
CC Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25437;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = (E)-beta-farnesene +
CC diphosphate; Xref=Rhea:RHEA:27425, ChEBI:CHEBI:10418,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:175763; EC=4.2.3.47;
CC Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:27426;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = beta-sesquiphellandrene +
CC diphosphate; Xref=Rhea:RHEA:32699, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:64361, ChEBI:CHEBI:175763; EC=4.2.3.123;
CC Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32700;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = (1S,5S,6R)-alpha-bergamotene +
CC diphosphate; Xref=Rhea:RHEA:31427, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:62756, ChEBI:CHEBI:175763; EC=4.2.3.81;
CC Evidence={ECO:0000269|PubMed:25056927};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31428;
CC Evidence={ECO:0000269|PubMed:25056927};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:25056927};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000269|PubMed:25056927};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit.
CC {ECO:0000250|UniProtKB:A0A1C9J6A7};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=673 uM for Mg(2+) {ECO:0000269|PubMed:25056927};
CC KM=35 uM for Mn(2+) {ECO:0000269|PubMed:25056927};
CC KM=20.54 uM for (2E)-geranyl diphosphate
CC {ECO:0000269|PubMed:25056927};
CC Vmax=1272.33 pmol/sec/mg enzyme with (2E)-geranyl diphosphate as
CC substrate {ECO:0000269|PubMed:25056927};
CC Note=kcat is 0.088 sec(-1) with (2E)-geranyl diphosphate as
CC substrate. {ECO:0000269|PubMed:25056927};
CC pH dependence:
CC Optimum pH is 7.5 (PubMed:25056927). Active at pH between 6.0 and 9.0
CC (PubMed:25056927). {ECO:0000269|PubMed:25056927};
CC Temperature dependence:
CC Optimum temperature is 30 degrees Celsius.
CC {ECO:0000269|PubMed:25056927};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000269|PubMed:25056927}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:25056927}.
CC -!- TISSUE SPECIFICITY: Highly expressed in flowers, petals and sepals, but
CC almost undetectable in vegetative organs.
CC {ECO:0000269|PubMed:25056927}.
CC -!- DEVELOPMENTAL STAGE: During flower development, Progressive accumlation
CC in petals during the first 40 hours, peaking at full bloom, and fades
CC out from 40 to 64 hours. {ECO:0000269|PubMed:25056927}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000250|UniProtKB:Q40577}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily.
CC {ECO:0000305}.
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DR EMBL; KF358246; AGY49283.1; -; mRNA.
DR UniPathway; UPA00213; -.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0034008; F:R-linalool synthase activity; IDA:UniProtKB.
DR GO; GO:0034007; F:S-linalool synthase activity; IDA:UniProtKB.
DR GO; GO:0010333; F:terpene synthase activity; IDA:UniProtKB.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR GO; GO:0010597; P:green leaf volatile biosynthetic process; IDA:UniProtKB.
DR GO; GO:0016099; P:monoterpenoid biosynthetic process; IDA:UniProtKB.
DR GO; GO:0051762; P:sesquiterpene biosynthetic process; IDA:UniProtKB.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Metal-binding; Plastid; Transit peptide.
FT TRANSIT 1..46
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 47..591
FT /note="Monoterpene synthase 8, chloroplastic"
FT /id="PRO_0000454955"
FT MOTIF 346..350
FT /note="DDXXD motif"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 346
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 346
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 350
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 350
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 497
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
SQ SEQUENCE 591 AA; 68357 MW; A3288B647A1DEDCC CRC64;
MSLLLAPPSY FPFRGLRRST AAKQPPCLRL VKCTADRQSP EAARRSAHYQ PNMWSDDYIQ
SLTVESPLKV EEKEQTKKLM LLKERIAEVI CEGKEVEEQL RLIDHLQQLG VAYHFKDDIK
ASLRNIHSSL EEISSTIIFK DGLHASALLF RLLRENGFSI SEDIFEEFRD EKGQYFRSDG
LKNQTDQAML SLYEASYYEK DGEMVLQEAM ECTTKHLENL LEEEGSDLKL KEQAAHALEL
PLNWRMERLH ARWFIEACQR EVMVIDNPLL LEFAKLDFNA VQSIYKKELS ALSRWWTKLG
VVEKLPFARD RLTENYLWTV GWAFEPEHWS FRDAQTKGNC FVTMIDDVYD VYGTLDELEL
FTHVVDRWDI NAIDQLPDYM KILFLALFNT VNDDGYKVMK EKGLDVIPYL KRSWADLCKA
YLVEAKWYHR GYKPTINEYL DNTWISISGP AIFTNAYCMA NNLTKQDLER FSEYPAIAKH
SSMLGRLYND LATSTAEIER GDVPKSIQCC MHERGVSEGV AREQVKELIR GNWRCMNGDR
AAASSFEEML KTVAVDIARA SQFFYHNGDK YGKADGETMT QVMSLLINPI I
