TPSBE_ZINZE
ID TPSBE_ZINZE Reviewed; 554 AA.
AC B1B1U4;
DT 21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=Beta-eudesmol synthase;
DE EC=4.2.3.68;
DE AltName: Full=10-epi-gamma-eudesmol synthase;
DE EC=4.2.3.84;
DE AltName: Full=Alpha-eudesmol synthase;
DE EC=4.2.3.85;
GN Name=ZSS2;
OS Zingiber zerumbet (Shampoo ginger) (Amomum zerumbet).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Zingiberales; Zingiberaceae;
OC Zingiber.
OX NCBI_TaxID=311405;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY,
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=18242187; DOI=10.1016/j.febslet.2008.01.020;
RA Yu F., Harada H., Yamasaki K., Okamoto S., Hirase S., Tanaka Y., Misawa N.,
RA Utsumi R.;
RT "Isolation and functional characterization of a beta-eudesmol synthase, a
RT new sesquiterpene synthase from Zingiber zerumbet Smith.";
RL FEBS Lett. 582:565-572(2008).
RN [2]
RP FUNCTION.
RX PubMed=15586673; DOI=10.1023/b:joec.0000042400.14451.08;
RA Marsaro A.L., Souza R.C., Della Lucia T.M., Fernandes J.B., Silva M.F.,
RA Vieira P.C.;
RT "Behavioral changes in workers of the leaf-cutting ant Atta sexdens
RT rubropilosa induced by chemical components of Eucalyptus maculata leaves.";
RL J. Chem. Ecol. 30:1771-1780(2004).
CC -!- FUNCTION: Involved in the biosynthesis of beta-eudesmol, a
CC sesquiterpene with antifungal activity and responsible for resistance
CC of plants to ant attack. Produces mainly beta-eudesmol, but also
CC smaller amounts of 10-epi-gamma-eudesmol, alpha-eudesmol and
CC aristolene. {ECO:0000269|PubMed:15586673, ECO:0000269|PubMed:18242187}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate + H2O = beta-eudesmol +
CC diphosphate; Xref=Rhea:RHEA:29495, ChEBI:CHEBI:10417,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:33019, ChEBI:CHEBI:175763;
CC EC=4.2.3.68; Evidence={ECO:0000269|PubMed:18242187};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate + H2O = 10-epi-gamma-eudesmol +
CC diphosphate; Xref=Rhea:RHEA:30947, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:62514, ChEBI:CHEBI:175763;
CC EC=4.2.3.84; Evidence={ECO:0000269|PubMed:18242187};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate + H2O = alpha-eudesmol +
CC diphosphate; Xref=Rhea:RHEA:30951, ChEBI:CHEBI:10278,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:33019, ChEBI:CHEBI:175763;
CC EC=4.2.3.85; Evidence={ECO:0000269|PubMed:18242187};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000305};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000305};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in rhizomes. Detected in stems, but not
CC in leaves. {ECO:0000269|PubMed:18242187}.
CC -!- INDUCTION: Peak of expression in summer. {ECO:0000269|PubMed:18242187}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR EMBL; AB247334; BAG12021.1; -; mRNA.
DR EMBL; AB263738; BAG12022.1; -; Genomic_DNA.
DR AlphaFoldDB; B1B1U4; -.
DR SMR; B1B1U4; -.
DR KEGG; ag:BAG12021; -.
DR BioCyc; MetaCyc:MON-14903; -.
DR BRENDA; 4.2.3.68; 12510.
DR BRENDA; 4.2.3.84; 12510.
DR BRENDA; 4.2.3.85; 12510.
DR UniPathway; UPA00213; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Lyase; Magnesium; Manganese; Metal-binding; Plant defense.
FT CHAIN 1..554
FT /note="Beta-eudesmol synthase"
FT /id="PRO_0000412250"
FT MOTIF 305..309
FT /note="DDXXD motif"
FT BINDING 305
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 305
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 309
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 309
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 456
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
SQ SEQUENCE 554 AA; 64382 MW; 3F8E932949B9EA22 CRC64;
MEKQSLTFDG DEEAKIDRKS SKYHPSIWGD YFIQNSSLTH AKESTQRMIK RVEELKVQVK
SMFKDTSDLL QLMNLINSIQ MLGLDYHFEN EIDEALRLIY EVDDKSYGLY ETSLRFQLLR
QHGYHVSADI FNKFKDDNGS FISSLNGDAK GLLSLYNVSY LGTHGETILD EAKSFTKPQL
VSLMSELEQS LAAQVSLFLE LPLCRRNKIL LARKYILIYQ EDAMRNNVIL ELAKLNFNLL
QSLYQEELKK ISIWWNDLAF AKSLSFTRDR VVEGYYWVLT IYFEPQHSRA RVICSKVFAF
LSIMDDIYDN YGILEECTLL TEAIKRWNPQ AIDGLPEYLK DYYLKLLKTF EEFEDELELN
EKYRMLYLQD EVKALAISYL QEAKWGIERH VPSLDEHLHN SLISSGSSTV ICASFVGMGE
VATKEVFDWL SSFPKVVEAC CVIGRLLNDI RSHELEQGRD HTASTVESYM KEHDTNVDVA
CEKLREIVEK AWKDLNNESL NPTKVPRLMI ERIVNLSKSN EEIYKYNDTY TNSDTTMKDN
ISLVLVESCD YFNK
