TPSD6_ABIGR
ID TPSD6_ABIGR Reviewed; 618 AA.
AC Q948Z0;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Camphene synthase, chloroplastic;
DE EC=4.2.3.117;
DE AltName: Full=(-)-(1S,4R)-camphene synthase;
DE AltName: Full=Agg-cam;
DE Flags: Precursor;
GN Name=ag6; Synonyms=AG6.5;
OS Abies grandis (Grand fir) (Pinus grandis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Abies.
OX NCBI_TaxID=46611;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9268308; DOI=10.1074/jbc.272.35.21784;
RA Bohlmann J., Steele C.L., Croteau R.B.;
RT "Monoterpene synthases from grand fir (Abies grandis). cDNA isolation,
RT characterization, and functional expression of myrcene synthase, (-)-(4S)-
RT limonene synthase, and (-)-(1S,5S)-pinene synthase.";
RL J. Biol. Chem. 272:21784-21792(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC TISSUE=Stem;
RX PubMed=10441373; DOI=10.1006/abbi.1999.1332;
RA Bohlmann J., Phillips M., Ramachandiran V., Katoh S., Croteau R.B.;
RT "cDNA cloning, characterization, and functional expression of four new
RT monoterpene synthase members of the Tpsd gene family from grand fir (Abies
RT grandis).";
RL Arch. Biochem. Biophys. 368:232-243(1999).
RN [3]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=9539701; DOI=10.1073/pnas.95.8.4126;
RA Bohlmann J., Meyer-Gauen G., Croteau R.B.;
RT "Plant terpenoid synthases: molecular biology and phylogenetic analysis.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:4126-4133(1998).
RN [4]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=11404343; DOI=10.1093/genetics/158.2.811;
RA Trapp S.C., Croteau R.B.;
RT "Genomic organization of plant terpene synthases and molecular evolutionary
RT implications.";
RL Genetics 158:811-832(2001).
CC -!- FUNCTION: Involved in defensive oleoresin formation in conifers in
CC response to insect attack or other injury. Involved in monoterpene
CC (C10) olefins biosynthesis. {ECO:0000269|PubMed:10441373,
CC ECO:0000269|PubMed:11404343, ECO:0000269|PubMed:9539701}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = (1S,4R)-camphene + diphosphate;
CC Xref=Rhea:RHEA:25484, ChEBI:CHEBI:89, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58057; EC=4.2.3.117;
CC Evidence={ECO:0000269|PubMed:10441373};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC -!- COFACTOR:
CC Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000250};
CC -!- PATHWAY: Terpene metabolism; oleoresin biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- MISCELLANEOUS: The conserved 59-Arg-Arg-60 motif may play a role in the
CC isomerization step of the terpenoid cyclization reaction sequence.
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsd subfamily.
CC {ECO:0000305}.
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DR EMBL; U87910; AAB70707.1; -; mRNA.
DR AlphaFoldDB; Q948Z0; -.
DR SMR; Q948Z0; -.
DR PRIDE; Q948Z0; -.
DR KEGG; ag:AAB70707; -.
DR BioCyc; MetaCyc:AG6-MON; -.
DR BRENDA; 4.2.3.117; 2.
DR BRENDA; 4.2.3.119; 2.
DR UniPathway; UPA00924; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0102703; F:camphene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Manganese; Metal-binding; Plastid;
KW Transit peptide.
FT TRANSIT 1..51
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 52..618
FT /note="Camphene synthase, chloroplastic"
FT /id="PRO_0000033629"
FT MOTIF 369..373
FT /note="DDXXD motif"
FT BINDING 369
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 369
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 373
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 373
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 521
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
SQ SEQUENCE 618 AA; 70750 MW; B07B5185CE5C4CE1 CRC64;
MALLSITPLV SRSCLSSSHE IKALRRTIPT LGICRPGKSV AHSINMCLTS VASTDSVQRR
VGNYHSNLWD DDFIQSLIST PYGAPDYRER ADRLIGEVKD IMFNFKSLED GGNDLLQRLL
LVDDVERLGI DRHFKKEIKT ALDYVNSYWN EKGIGCGRES VVTDLNSTAL GLRTLRLHGY
TVSSDVLNVF KDKNGQFSST ANIQIEGEIR GVLNLFRASL VAFPGEKVMD EAETFSTKYL
REALQKIPAS SILSLEIRDV LEYGWHTNLP RLEARNYMDV FGQHTKNKNA AEKLLELAKL
EFNIFHSLQE RELKHVSRWW KDSGSPEMTF CRHRHVEYYA LASCIAFEPQ HSGFRLGFTK
MSHLITVLDD MYDVFGTVDE LELFTATIKR WDPSAMECLP EYMKGVYMMV YHTVNEMARV
AEKAQGRDTL NYARQAWEAC FDSYMQEAKW IATGYLPTFE EYLENGKVSS AHRPCALQPI
LTLDIPFPDH ILKEVDFPSK LNDLICIILR LRGDTRCYKA DRARGEEASS ISCYMKDNPG
LTEEDALNHI NFMIRDAIRE LNWELLKPDN SVPITSKKHA FDISRVWHHG YRYRDGYSFA
NVETKSLVMR TVIEPVPL
