TPSDB_ABIGR
ID TPSDB_ABIGR Reviewed; 637 AA.
AC Q9M7C9;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Limonene/alpha-pinene synthase, chloroplastic;
DE AltName: Full=(-)-(1S,5S)-alpha-pinene synthase;
DE EC=4.2.3.119;
DE AltName: Full=(-)-(4S)-limonene synthase;
DE EC=4.2.3.16;
DE AltName: Full=Agg-pin2;
DE Flags: Precursor;
GN Name=ag11; Synonyms=agc11;
OS Abies grandis (Grand fir) (Pinus grandis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Abies.
OX NCBI_TaxID=46611;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC TISSUE=Stem;
RX PubMed=10441373; DOI=10.1006/abbi.1999.1332;
RA Bohlmann J., Phillips M., Ramachandiran V., Katoh S., Croteau R.B.;
RT "cDNA cloning, characterization, and functional expression of four new
RT monoterpene synthase members of the Tpsd gene family from grand fir (Abies
RT grandis).";
RL Arch. Biochem. Biophys. 368:232-243(1999).
RN [2]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=9539701; DOI=10.1073/pnas.95.8.4126;
RA Bohlmann J., Meyer-Gauen G., Croteau R.B.;
RT "Plant terpenoid synthases: molecular biology and phylogenetic analysis.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:4126-4133(1998).
RN [3]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=11404343; DOI=10.1093/genetics/158.2.811;
RA Trapp S.C., Croteau R.B.;
RT "Genomic organization of plant terpene synthases and molecular evolutionary
RT implications.";
RL Genetics 158:811-832(2001).
CC -!- FUNCTION: Involved in defensive oleoresin formation in conifers in
CC response to insect attack or other injury. Involved in monoterpene
CC (C10) olefins biosynthesis. {ECO:0000269|PubMed:10441373,
CC ECO:0000269|PubMed:11404343, ECO:0000269|PubMed:9539701}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = (4S)-limonene + diphosphate;
CC Xref=Rhea:RHEA:12869, ChEBI:CHEBI:15383, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58057; EC=4.2.3.16;
CC Evidence={ECO:0000269|PubMed:10441373};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = (1S,5S)-alpha-pinene + diphosphate;
CC Xref=Rhea:RHEA:25488, ChEBI:CHEBI:28660, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58057; EC=4.2.3.119;
CC Evidence={ECO:0000269|PubMed:10441373};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC -!- COFACTOR:
CC Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000250};
CC -!- PATHWAY: Terpene metabolism; oleoresin biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC -!- DOMAIN: The C-terminal part of the protein seems to be involved in the
CC cyclization step(s) required for the synthesis of pinene.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- MISCELLANEOUS: The conserved 70-Arg-Arg-71 motif may play a role in the
CC isomerization step of the terpenoid cyclization reaction sequence.
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsd subfamily.
CC {ECO:0000305}.
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DR EMBL; AF139207; AAF61455.1; -; mRNA.
DR AlphaFoldDB; Q9M7C9; -.
DR SMR; Q9M7C9; -.
DR KEGG; ag:AAF61455; -.
DR BioCyc; MetaCyc:AG11-MON; -.
DR BRENDA; 4.2.3.119; 2.
DR UniPathway; UPA00924; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0050552; F:(4S)-limonene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Manganese; Metal-binding;
KW Multifunctional enzyme; Plastid; Transit peptide.
FT TRANSIT 1..56
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 57..637
FT /note="Limonene/alpha-pinene synthase, chloroplastic"
FT /id="PRO_0000033633"
FT MOTIF 388..392
FT /note="DDXXD motif"
FT BINDING 388
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 388
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 392
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 392
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 540
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
SQ SEQUENCE 637 AA; 73273 MW; B3574986FEC96CFB CRC64;
MALLSIVSLQ VPKSCGLKSL ISSSNVQKAL CISTAVPTLR MRRRQKALVI NMKLTTVSHR
DDNGGGVLQR RIADHHPNLW EDDFIQSLSS PYGGSSYSER AVTVVEEVKE MFNSIPNNRE
LFGSQNDLLT RLWMVDSIER LGIDRHFQNE IRVALDYVYS YWKEKEGIGC GRDSTFPDLN
STALALRTLR LHGYNVSSDV LEYFKDQKGH FACPAILTEG QITRSVLNLY RASLVAFPGE
KVMEEAEIFS ASYLKEVLQK IPVSSFSREI EYVLEYGWHT NLPRLEARNY IDVYGQDSYE
SSNEMPYVNT QKLLKLAKLE FNIFHSLQQK ELQYISRWWK DSCSSHLTFT RHRHVEYYTM
ASCISMEPKH SAFRLGFVKT CHLLTVLDDM YDTFGTLDEL QLFTTAFKRW DLSETKCLPE
YMKAVYMDLY QCLNELAQEA EKTQGRDTLN YIRNAYESHF DSFMHEAKWI SSGYLPTFEE
YLKNGKVSSG SRTATLQPIL TLDVPLPNYI LQEIDYPSRF NDLASSLLRL RGDTRCYKAD
RARGEEASAI SCYMKDHPGS TEEDALNHIN VMISDAIREL NWELLRPDSK SPISSKKHAF
DITRAFHHLY KYRDGYTVAS SETKNLVMKT VLEPVAL
