ID   TPT_MAIZE               Reviewed;         409 AA.
AC   P49133;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Triose phosphate/phosphate translocator, chloroplastic;
DE            Short=cTPT;
DE   Flags: Precursor;
GN   Name=TPT;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 305-316.
RC   TISSUE=Leaf;
RX   PubMed=8148878; DOI=10.1046/j.1365-313x.1994.05020215.x;
RA   Fischer K., Arbinger B., Kammerer B., Busch C., Brink S., Wallmeier H.,
RA   Sauer N., Eckerskorn C., Fluegge U.-I.;
RT   "Cloning and in vivo expression of functional triose phosphate/phosphate
RT   translocators from C3- and C4-plants: evidence for the putative
RT   participation of specific amino acid residues in the recognition of
RT   phosphoenolpyruvate.";
RL   Plant J. 5:215-226(1994).
CC   -!- FUNCTION: Mediates the export of fixed carbons from the chloroplasts
CC       into the cytosol in the form of triose phosphates. In addition, it can
CC       also bind and transport phosphoenolpyruvate, thereby increasing the
CC       photosynthetic efficiency of C4-plants.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane; Multi-pass
CC       membrane protein. Note=Located in zones of contact between the inner
CC       and outer membrane of the chloroplast.
CC   -!- SIMILARITY: Belongs to the TPT transporter family. TPT (TC 2.A.7.9)
CC       subfamily. {ECO:0000305}.
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DR   EMBL; Z26595; CAA81349.1; -; mRNA.
DR   PIR; S37497; S37497.
DR   RefSeq; NP_001105497.1; NM_001112027.1.
DR   AlphaFoldDB; P49133; -.
DR   SMR; P49133; -.
DR   STRING; 4577.GRMZM2G070605_P01; -.
DR   TCDB; 2.A.7.9.1; the drug/metabolite transporter (dmt) superfamily.
DR   PRIDE; P49133; -.
DR   EnsemblPlants; Zm00001eb118870_T001; Zm00001eb118870_P001; Zm00001eb118870.
DR   GeneID; 542473; -.
DR   Gramene; Zm00001eb118870_T001; Zm00001eb118870_P001; Zm00001eb118870.
DR   KEGG; zma:542473; -.
DR   MaizeGDB; 86189; -.
DR   eggNOG; KOG1441; Eukaryota.
DR   OMA; ASLRIPC; -.
DR   OrthoDB; 1453018at2759; -.
DR   Proteomes; UP000007305; Chromosome 3.
DR   ExpressionAtlas; P49133; baseline and differential.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR   GO; GO:0008514; F:organic anion transmembrane transporter activity; IEA:UniProt.
DR   GO; GO:0015605; F:organophosphate ester transmembrane transporter activity; IEA:UniProt.
DR   GO; GO:0015718; P:monocarboxylic acid transport; IEA:UniProt.
DR   InterPro; IPR004853; Sugar_P_trans_dom.
DR   InterPro; IPR004696; Tpt_PEP_transl.
DR   Pfam; PF03151; TPT; 1.
DR   TIGRFAMs; TIGR00817; tpt; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Direct protein sequencing; Membrane; Plastid;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix;
KW   Transport.
FT   TRANSIT         1..85
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           86..409
FT                   /note="Triose phosphate/phosphate translocator,
FT                   chloroplastic"
FT                   /id="PRO_0000035707"
FT   TOPO_DOM        86..104
FT                   /note="Chloroplast intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        105..125
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        126..137
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..158
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        159..215
FT                   /note="Chloroplast intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        216..236
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        237..280
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        281..300
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        301..378
FT                   /note="Chloroplast intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        379..399
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        400..409
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   SITE            225
FT                   /note="May be essential for binding and transport of
FT                   phosphoenolpyruvate"
SQ   SEQUENCE   409 AA;  44090 MW;  8C33700CCDB3D326 CRC64;
     MSALGTLSGG AAGVSGLLRL RRRAAPAPAI AAPSHLPAGT VKCAAAVPDA APIVWGRQLR
     PALLLPAALL PSLQPARRHT LQPPAAAAES AGEAKSVGFL EKYPALVTGF FFFMWYFLNV
     IFNILNKKIY NYFPYPYFVS LIHLVVGVVY CLISWSVGLP KRAPINGTLL KLLFPVALCH
     GIGHITSNVS FAAVAVSFAH TIKALEPFFS AAATQFILGQ QVPFSLWLSL APVVIGVSMA
     SLTELSFNWT GFINAMISNI SFTYRSIYSK KAMTDMDSTN VYAYISIIAL IVCIPPALIF
     EGPKLMQHGF SDAIAKVGLT KFVSDLFLVG LFYHLYNQIA TNTLERVAPL THAVGNVLKR
     VFVIGFSIIV FGNKISTQTG IGTSIAIAGV AMYSYIKAKI EEEKRKKSA