ID   TPT_PEA                 Reviewed;         402 AA.
AC   P21727;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Triose phosphate/phosphate translocator, chloroplastic;
DE            Short=cTPT;
DE   AltName: Full=E30;
DE   AltName: Full=p36;
DE   Flags: Precursor;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 73-98; 249-260; 269-289
RP   AND 324-350.
RC   STRAIN=cv. Progress No. 9; TISSUE=Seedling;
RX   PubMed=2172258; DOI=10.1083/jcb.111.5.1825;
RA   Schnell D.J., Blobel G., Pain D.;
RT   "The chloroplast import receptor is an integral membrane protein of
RT   chloroplast envelope contact sites.";
RL   J. Cell Biol. 111:1825-1838(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 73-82.
RC   TISSUE=Leaf;
RA   Willey D.L., Fischer K., Wachter E., Link T.A., Fluegge U.-I.;
RT   "Molecular cloning and structural analysis of the phosphate translocator
RT   from pea chloroplasts and its comparison to the spinach phosphate
RT   translocator.";
RL   Planta 183:451-461(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Weber A.;
RL   Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mediates the export of fixed carbons from the chloroplasts
CC       into the cytosol in the form of triose phosphates.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane; Multi-pass
CC       membrane protein. Note=Located in zones of contact between the inner
CC       and outer membrane of the chloroplast.
CC   -!- SIMILARITY: Belongs to the TPT transporter family. TPT (TC 2.A.7.9)
CC       subfamily. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to function as a chloroplast protein
CC       import receptor. {ECO:0000305}.
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DR   EMBL; X54639; CAA38451.1; -; mRNA.
DR   EMBL; X68077; CAA48210.1; -; mRNA.
DR   PIR; S23774; S23774.
DR   AlphaFoldDB; P21727; -.
DR   SMR; P21727; -.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015605; F:organophosphate ester transmembrane transporter activity; IEA:UniProt.
DR   GO; GO:0015120; F:phosphoglycerate transmembrane transporter activity; IEA:UniProt.
DR   InterPro; IPR004853; Sugar_P_trans_dom.
DR   InterPro; IPR004696; Tpt_PEP_transl.
DR   Pfam; PF03151; TPT; 1.
DR   TIGRFAMs; TIGR00817; tpt; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Direct protein sequencing; Membrane; Plastid; Transit peptide;
KW   Transmembrane; Transmembrane helix; Transport.
FT   TRANSIT         1..72
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000269|PubMed:2172258, ECO:0000269|Ref.2"
FT   CHAIN           73..402
FT                   /note="Triose phosphate/phosphate translocator,
FT                   chloroplastic"
FT                   /id="PRO_0000035708"
FT   TOPO_DOM        73..96
FT                   /note="Chloroplast intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        118..129
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        130..150
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        151..207
FT                   /note="Chloroplast intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        208..228
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        229..272
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        273..292
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        293..370
FT                   /note="Chloroplast intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        371..391
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        392..402
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   VARIANT         227
FT                   /note="I -> L"
SQ   SEQUENCE   402 AA;  43649 MW;  D6EF731F3CEF7600 CRC64;
     MESRVLSRAT TLSSLPTLNK LHRLPLANAS LPSVKSFGSV SDGGNLVWGR QLRPELCSPV
     LKKGASLLRP CPATAGGNDS AGEEKVAPVG FFSRYPALTT GFFFFTWYFL NVIFNILNKK
     IYNYFPYPYF VSVIHLAVGV VYCLVSWTVG LPKRAPIDGN LLKLLIPVAV CHALGHVTSN
     VSFAAVAVSF THTVKALEPF FNAAASQFIL GQSIPITLWL SLAPVVIGVS MASLTELSFN
     WLGFISAMIS NISFTYRSIY SKKAMTDMDS TNIYAYISII ALIVCIPPAL IIEGPTLLKT
     GFNDAIAKVG LVKFVSDLFW VGMFYHLYNQ VATNTLERVA PLTHAVGNVL KRVFVIGFSI
     IIFGNKISTQ TGIGTGIAIA GVALYSFIKA QIEEEKRQAK AA