ID   TPT_SPIOL               Reviewed;         404 AA.
AC   P11869;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Triose phosphate/phosphate translocator, chloroplastic;
DE            Short=cTPT;
DE   AltName: Full=E29;
DE   AltName: Full=p36;
DE   Flags: Precursor;
OS   Spinacia oleracea (Spinach).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX   NCBI_TaxID=3562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Leaf;
RX   PubMed=2714257; DOI=10.1002/j.1460-2075.1989.tb03346.x;
RA   Fluegge U.-I., Fischer K., Gross A., Lottspeich F., Eckerskorn C.,
RA   Sebald W.;
RT   "The triose phosphate-3-phosphoglycerate-phosphate translocator from
RT   spinach chloroplasts: nucleotide sequence of a full-length cDNA clone and
RT   import of the in vitro synthesized precursor protein into chloroplasts.";
RL   EMBO J. 8:39-46(1989).
RN   [2]
RP   PROTEIN SEQUENCE OF 91-99, AND FUNCTION.
RA   Fluegge U.-I., Weber A., Fischer K., Lottspeich F., Eckerskorn C.,
RA   Waegemann K., Soll J.;
RT   "The major chloroplast envelope polypeptide is the phosphate translocator
RT   and not the protein import receptor.";
RL   Nature 353:364-367(1991).
CC   -!- FUNCTION: Mediates the export of fixed carbons from the chloroplasts
CC       into the cytosol in the form of triose phosphates. {ECO:0000269|Ref.2}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane; Multi-pass
CC       membrane protein. Note=Located in zones of contact between the inner
CC       and outer membrane of the chloroplast.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the TPT transporter family. TPT (TC 2.A.7.9)
CC       subfamily. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to function as a chloroplast protein
CC       import receptor. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X13754; CAA32016.1; -; mRNA.
DR   PIR; S03638; S03638.
DR   AlphaFoldDB; P11869; -.
DR   SMR; P11869; -.
DR   OrthoDB; 1453018at2759; -.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015605; F:organophosphate ester transmembrane transporter activity; IEA:UniProt.
DR   GO; GO:0015120; F:phosphoglycerate transmembrane transporter activity; IEA:UniProt.
DR   InterPro; IPR004853; Sugar_P_trans_dom.
DR   InterPro; IPR004696; Tpt_PEP_transl.
DR   Pfam; PF03151; TPT; 1.
DR   TIGRFAMs; TIGR00817; tpt; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Direct protein sequencing; Membrane; Plastid; Transit peptide;
KW   Transmembrane; Transmembrane helix; Transport.
FT   TRANSIT         1..74
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000250"
FT   CHAIN           75..404
FT                   /note="Triose phosphate/phosphate translocator,
FT                   chloroplastic"
FT                   /id="PRO_0000035710"
FT   TOPO_DOM        75..98
FT                   /note="Chloroplast intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        120..131
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        132..152
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        153..209
FT                   /note="Chloroplast intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        210..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        231..274
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        275..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        295..372
FT                   /note="Chloroplast intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        373..393
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        394..404
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   404 AA;  44234 MW;  20E726F081A24A04 CRC64;
     MESRVLSRTT AIAALPKLFR PSREAASFGF ATGVKTPVGL VKDGGSLTWG RQLRPVLLLE
     PVQTGPVCSR REKTAVQPCR AASGSSGEAK TGFLEKYPAL VTGSFFFMWY FLNVIFNILN
     KKIYNYFPYP YFVSVIHLFV GVVYCLASWS VGLPKRAPMD SKLLKLLIPV AVCHAIGHVT
     SNVSFAAVAV SFTHTIKALE PFFNAAASQF VLGQSIPITL WLSLAPVVIG VSMASLTELS
     FNWLGFISAM ISNVSFTYRS LYSKKAMTDM DSTNIYAYIS IIALFVCLPP AIIVEGPQLM
     KHGFNDAIAK VGLTKFISDL FWVGMFYHLY NQLATNTLER VAPLTHAVGN VLKRVFVIGF
     SIIAFGNKIS TQTAIGTSIA IAGVALYSLI KAKMEEEKRQ MKST