TPX2_XENTR
ID TPX2_XENTR Reviewed; 709 AA.
AC A4IH24;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=Targeting protein for Xklp2;
GN Name=tpx2;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Spindle assembly factor. Required for normal assembly of
CC mitotic spindles. Mediates the binding kif15 and aurka to spindle
CC microtubules. Required for targeting kif15 to microtubule minus ends.
CC Activates aurka by promoting its autophosphorylation and protects the
CC phosphorylated residue against dephosphorylation (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Associates with microtubules. Interacts with aurka and plk1.
CC Interacts with kif15 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm, cytoskeleton,
CC spindle {ECO:0000250}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000250}. Note=Localizes during metaphase on spindle microtubules,
CC with a strong enrichment at spindle poles. Localizes to the minus ends
CC of spindle pole and aster microtubules in a dynein- and dynactin-
CC dependent manner (By similarity). {ECO:0000250}.
CC -!- PTM: Phosphorylated during mitosis. Hyperphosphorylated upon assembly
CC of microtubules (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TPX2 family. {ECO:0000305}.
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DR EMBL; BC135342; AAI35343.1; -; mRNA.
DR RefSeq; NP_001096233.1; NM_001102763.1.
DR AlphaFoldDB; A4IH24; -.
DR SMR; A4IH24; -.
DR STRING; 8364.ENSXETP00000034886; -.
DR PaxDb; A4IH24; -.
DR DNASU; 100124787; -.
DR GeneID; 100124787; -.
DR KEGG; xtr:100124787; -.
DR CTD; 22974; -.
DR Xenbase; XB-GENE-5810264; tpx2.
DR eggNOG; ENOG502QVQS; Eukaryota.
DR InParanoid; A4IH24; -.
DR OrthoDB; 1390734at2759; -.
DR Reactome; R-XTR-8854518; AURKA Activation by TPX2.
DR Proteomes; UP000008143; Chromosome 10.
DR Proteomes; UP000790000; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR GO; GO:0005880; C:nuclear microtubule; IBA:GO_Central.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0030295; F:protein kinase activator activity; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0090307; P:mitotic spindle assembly; IBA:GO_Central.
DR GO; GO:0060236; P:regulation of mitotic spindle organization; IEA:InterPro.
DR InterPro; IPR015128; Aurora-A-bd.
DR InterPro; IPR027329; TPX2_C.
DR InterPro; IPR027330; TPX2_central_dom.
DR InterPro; IPR009675; TPX2_fam.
DR PANTHER; PTHR14326; PTHR14326; 1.
DR Pfam; PF09041; Aurora-A_bind; 1.
DR Pfam; PF06886; TPX2; 3.
DR Pfam; PF12214; TPX2_importin; 2.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Microtubule; Mitosis;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..709
FT /note="Targeting protein for Xklp2"
FT /id="PRO_0000393116"
FT REGION 40..189
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 229..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 309..347
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 641..668
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..85
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..105
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 106..121
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 144..158
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..189
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 230..249
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 206
FT /note="Phosphoserine; by plk1"
FT /evidence="ECO:0000250"
SQ SEQUENCE 709 AA; 81373 MW; 8DC9E282DC4F267D CRC64;
MAEAQDLYTF DAPSTFINFT AFHEDHGADS WFDKVTNAEN IPPDQRRLSE IPAVNAEQNG
MVEPEETSPS KETVSESAAH FSDGKSQARR SSRRMSRKHR QKLLVKMRET RLEKETAQSD
CPPTKKLKGS STKGARTPVI RGQPRSGHGS NTSPRPKAPL TLPSTPTVLK RKNVMVKPKS
SEEQELERMQ ELQKEMLENL KKNEHSMKAA ISGTGPSVKK SAVPITKPVD FHFKTDDRPK
RVADQPKGEE YKEVDFAAAL RKHPPSPVSK AALTVPKPFN LSKGKRKHEE ASEFVSTAEQ
VISFSKKTPA RYHLRSRQRE LEGPSPVKMV RPKLTNPKTP LLQTKQRLRP VMCKSAAELE
AEELERIQQY KFKAQELDSR ILEGAQVLPR KPPVKEPTKA IGFDLEIEKR IQQREKRDEG
EEEAFTFHSR PCPSKLLTEV VGVPLKKLLP VTVPHSPSFA LKNRVRLPAR EEKEEEVPAI
KATAMPHYGV PFRPKLVQQR QVEVCPFSFS DQDRERLLRK EKRMEELRKE EVHKFKAQPL
PEFGHVSLPE KRVKMPTQQE PFVLEIDKRG APRLQRWQQQ VKEEQKQQKE MAVFKARPNT
VVHQEPFLPK KESRCLTATE GFELATEKRA KERQEFEKSL AEMEAQKSLL EEETRQRQEE
EEREEISHLR KELVHKAQPI RKYKAVDVKA SDTPLTIPES PNFSDRFKC
