TR103_MOUSE
ID TR103_MOUSE Reviewed; 312 AA.
AC Q9JKA3; Q7M706;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Taste receptor type 2 member 103;
DE Short=T2R103;
DE AltName: Full=Taste receptor family B member 2;
DE Short=TRB2;
DE AltName: Full=Taste receptor type 2 member 10;
DE Short=T2R10;
GN Name=Tas2r103; Synonyms=Tas2r10;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 36-277.
RC STRAIN=DBA/2J;
RX PubMed=10766242; DOI=10.1038/35007072;
RA Matsunami H., Montmayeur J.-P., Buck L.B.;
RT "A family of candidate taste receptors in human and mouse.";
RL Nature 404:601-604(2000).
RN [3]
RP IDENTIFICATION.
RX PubMed=12679530; DOI=10.1093/molbev/msg083;
RA Shi P., Zhang J., Yang H., Zhang Y.-P.;
RT "Adaptive diversification of bitter taste receptor genes in mammalian
RT evolution.";
RL Mol. Biol. Evol. 20:805-814(2003).
RN [4]
RP REVIEW.
RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [5]
RP REVIEW.
RX PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [6]
RP REVIEW.
RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
CC -!- FUNCTION: Gustducin-coupled receptor implicated in the perception of
CC bitter compounds in the oral cavity and the gastrointestinal tract.
CC Signals through PLCB2 and the calcium-regulated cation channel TRPM5.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC tongue and palate epithelium and exclusively in gustducin-positive
CC cells. Expressed in 15% taste bud cells in circumvallate and foliate
CC papillae but only in 2% in fungiform papillae.
CC -!- MISCELLANEOUS: Several bitter taste receptors are expressed in a single
CC taste receptor cell.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC152822; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AF247732; AAF64509.1; -; Genomic_DNA.
DR EMBL; BK001091; DAA01230.1; -; Genomic_DNA.
DR CCDS; CCDS51933.1; -.
DR RefSeq; NP_444441.1; NM_053211.1.
DR AlphaFoldDB; Q9JKA3; -.
DR SMR; Q9JKA3; -.
DR STRING; 10090.ENSMUSP00000032317; -.
DR GlyGen; Q9JKA3; 1 site.
DR PhosphoSitePlus; Q9JKA3; -.
DR PaxDb; Q9JKA3; -.
DR PRIDE; Q9JKA3; -.
DR Ensembl; ENSMUST00000032317; ENSMUSP00000032317; ENSMUSG00000030196.
DR GeneID; 667992; -.
DR KEGG; mmu:667992; -.
DR UCSC; uc012eug.1; mouse.
DR CTD; 667992; -.
DR MGI; MGI:1890257; Tas2r103.
DR VEuPathDB; HostDB:ENSMUSG00000030196; -.
DR eggNOG; ENOG502SKRK; Eukaryota.
DR GeneTree; ENSGT00960000186648; -.
DR HOGENOM; CLU_072337_3_0_1; -.
DR InParanoid; Q9JKA3; -.
DR OMA; AMTNTHI; -.
DR OrthoDB; 1053374at2759; -.
DR PhylomeDB; Q9JKA3; -.
DR TreeFam; TF335891; -.
DR BioGRID-ORCS; 667992; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q9JKA3; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9JKA3; protein.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0033038; F:bitter taste receptor activity; ISO:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0008527; F:taste receptor activity; ISO:MGI.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; ISO:MGI.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR007960; TAS2R.
DR Pfam; PF05296; TAS2R; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..312
FT /note="Taste receptor type 2 member 103"
FT /id="PRO_0000082243"
FT TOPO_DOM 1..6
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..61
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..92
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 114..132
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..185
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..229
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..264
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..312
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 166
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 52
FT /note="Y -> N (in Ref. 1; AAF64509)"
FT /evidence="ECO:0000305"
FT CONFLICT 72..75
FT /note="Missing (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 125
FT /note="V -> A (in Ref. 1; AAF64509)"
FT /evidence="ECO:0000305"
FT CONFLICT 129..130
FT /note="EA -> KV (in Ref. 1; AAF64509)"
FT /evidence="ECO:0000305"
FT CONFLICT 146
FT /note="I -> F (in Ref. 1; AAF64509)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="T -> S (in Ref. 1; AAF64509)"
FT /evidence="ECO:0000305"
FT CONFLICT 177..178
FT /note="GL -> RR (in Ref. 1; AAF64509)"
FT /evidence="ECO:0000305"
FT CONFLICT 189
FT /note="T -> I (in Ref. 1; AAF64509)"
FT /evidence="ECO:0000305"
FT CONFLICT 213
FT /note="C -> H (in Ref. 1; AAF64509)"
FT /evidence="ECO:0000305"
FT CONFLICT 248
FT /note="L -> V (in Ref. 1; AAF64509)"
FT /evidence="ECO:0000305"
FT CONFLICT 267..272
FT /note="LRSIGV -> FTEYRR (in Ref. 1; AAF64509)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 312 AA; 35156 MW; 25972A2299781111 CRC64;
MVLTIRAILW VTLITIISLE FIIGILGNVF IALVNIIDWV KRGKISAVDK TYMALAISRT
AFLLSLITGF LVSLLDPALL GMRTMVRLLT ISWMVTNHFS VWFATCLSIF YFLKIANFSN
SIFLVLKWEA KKVVSVTLVV SVIILIMNII VINKFTDRLQ VNTLQNCSTS NTLKDYGLFL
FISTGFTLTP FAVSLTMFLL LIFSLWRHLK NMCHSATGSR DVSTVAHIKG LQTVVTFLLL
YTAFVMSLLS ESLNINIQHT NLLSHFLRSI GVAFPTGHSC VLILGNSKLR QASLSVILWL
RYKYKHIENW GP
