TR116_MOUSE
ID TR116_MOUSE Reviewed; 305 AA.
AC Q7M713; Q9JKA1; Q9JKA4;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Taste receptor type 2 member 116;
DE Short=T2R116;
DE Short=TRB1;
DE Short=mT2R56;
DE AltName: Full=TRB4;
GN Name=Tas2r116 {ECO:0000312|MGI:MGI:1890258};
GN Synonyms=T2r56 {ECO:0000303|PubMed:12679530};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0000312|EMBL:AAF64511.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 32-276.
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAF64511.1}, and
RC DBA/2J {ECO:0000312|EMBL:AAF64508.1};
RX PubMed=10766242; DOI=10.1038/35007072;
RA Matsunami H., Montmayeur J.-P., Buck L.B.;
RT "A family of candidate taste receptors in human and mouse.";
RL Nature 404:601-604(2000).
RN [3] {ECO:0000305, ECO:0000312|EMBL:DAA01223.1}
RP IDENTIFICATION.
RX PubMed=12679530; DOI=10.1093/molbev/msg083;
RA Shi P., Zhang J., Yang H., Zhang Y.-P.;
RT "Adaptive diversification of bitter taste receptor genes in mammalian
RT evolution.";
RL Mol. Biol. Evol. 20:805-814(2003).
CC -!- FUNCTION: Putative taste receptor which may play a role in the
CC perception of bitterness. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Several bitter taste receptors are expressed in a single
CC taste receptor cell. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000255}.
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DR EMBL; AC129318; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AF247731; AAF64508.1; -; Genomic_DNA.
DR EMBL; AF247734; AAF64511.1; -; mRNA.
DR EMBL; BK001084; DAA01223.1; -; Genomic_DNA.
DR CCDS; CCDS20625.1; -.
DR RefSeq; NP_444442.1; NM_053212.1.
DR AlphaFoldDB; Q7M713; -.
DR SMR; Q7M713; -.
DR STRING; 10090.ENSMUSP00000032315; -.
DR GlyGen; Q7M713; 3 sites.
DR PaxDb; Q7M713; -.
DR PRIDE; Q7M713; -.
DR DNASU; 112408; -.
DR Ensembl; ENSMUST00000032315; ENSMUSP00000032315; ENSMUSG00000030194.
DR GeneID; 112408; -.
DR KEGG; mmu:112408; -.
DR UCSC; uc009ejr.1; mouse.
DR CTD; 112408; -.
DR MGI; MGI:1890258; Tas2r116.
DR VEuPathDB; HostDB:ENSMUSG00000030194; -.
DR eggNOG; ENOG502SKRK; Eukaryota.
DR GeneTree; ENSGT00960000186648; -.
DR HOGENOM; CLU_072337_3_0_1; -.
DR InParanoid; Q7M713; -.
DR OMA; WLRCRHK; -.
DR OrthoDB; 1010573at2759; -.
DR PhylomeDB; Q7M713; -.
DR TreeFam; TF335891; -.
DR BioGRID-ORCS; 112408; 4 hits in 72 CRISPR screens.
DR PRO; PR:Q7M713; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q7M713; protein.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0033038; F:bitter taste receptor activity; ISO:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0008527; F:taste receptor activity; ISO:MGI.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; ISO:MGI.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR007960; TAS2R.
DR Pfam; PF05296; TAS2R; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..305
FT /note="Taste receptor type 2 member 116"
FT /id="PRO_0000248475"
FT TOPO_DOM 1..8
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 9..29
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..101
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..184
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..236
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 258..261
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 283..305
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 93
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 160
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 170
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 47
FT /note="I -> S (in Ref. 2; AAF64508)"
FT /evidence="ECO:0000305"
FT CONFLICT 70
FT /note="V -> M (in Ref. 2; AAF64508)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 305 AA; 35095 MW; 0DD7D8631B850809 CRC64;
MNGVLQVTFI VILSVEFIIG IFGNGFIAVV NIKDLVKGRK ISSVDQILTA LAISRIALLW
LILVSWWIFV LYPGQWMTDR RVSIMHSIWT TFNQSSLWFA TSLSIFYFFK IANFSNPIFL
YLKVRLKKVM IGTLIMSLIL FCLNIIIMNA PENILITEYN VSMSYSLILN NTQLSMLFPF
ANTMFGFIPF AVSLVTFVLL VFSLWKHQRK MQHSAHGCRD ASTKAHIRAL QTLIASLLLY
SIFFLSHVMK VWSALLLERT LLLLITQVAR TAFPSVHSWV LILGNAKMRK ASLYVFLWLR
CRHKE
