TR120_ARATH
ID TR120_ARATH Reviewed; 1186 AA.
AC Q9FY61;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Trafficking protein particle complex II-specific subunit 120 homolog {ECO:0000305};
DE Short=AtTRS120 {ECO:0000303|PubMed:20713617};
DE Short=TRAPP II-specific subunit 120 homolog {ECO:0000305};
DE AltName: Full=Protein VASCULAR NETWORK DEFECTIVE 4 {ECO:0000303|PubMed:24443495};
GN Name=TRS120 {ECO:0000303|PubMed:20713617};
GN Synonyms=VAN4 {ECO:0000303|PubMed:24443495};
GN OrderedLocusNames=At5g11040 {ECO:0000312|Araport:AT5G11040};
GN ORFNames=T5K6.30 {ECO:0000312|EMBL:CAC03452.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-971, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [5]
RP FUNCTION, COMPONENT OF THE TRAPPII COMPLEX, AND DISRUPTION PHENOTYPE.
RX PubMed=20713617; DOI=10.1104/pp.110.154286;
RA Thellmann M., Rybak K., Thiele K., Wanner G., Assaad F.F.;
RT "Tethering factors required for cytokinesis in Arabidopsis.";
RL Plant Physiol. 154:720-732(2010).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21689172; DOI=10.1111/j.1365-313x.2011.04681.x;
RA Qi X., Kaneda M., Chen J., Geitmann A., Zheng H.;
RT "A specific role for Arabidopsis TRAPPII in post-Golgi trafficking that is
RT crucial for cytokinesis and cell polarity.";
RL Plant J. 68:234-248(2011).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=24443495; DOI=10.1093/pcp/pcu012;
RA Naramoto S., Nodzylski T., Dainobu T., Takatsuka H., Okada T., Friml J.,
RA Fukuda H.;
RT "VAN4 encodes a putative TRS120 that is required for normal cell growth and
RT vein development in Arabidopsis.";
RL Plant Cell Physiol. 55:750-763(2014).
CC -!- FUNCTION: Specific subunit of the TRAPP II complex, a highly conserved
CC vesicle tethering complex that is required for the proper transport of
CC proteins in post-Golgi trafficking pathways to the growing cell plate
CC in mitotic active cells (PubMed:20713617, PubMed:21689172,
CC PubMed:24443495). Required for the polarized and selective transport of
CC PIN2 and probably PIN1 to the plasma membrane (PubMed:21689172,
CC PubMed:24443495). Not required for ER-to-Golgi as well as biosynthetic
CC and endocytic vacuolar transport (PubMed:21689172).
CC {ECO:0000269|PubMed:20713617, ECO:0000269|PubMed:21689172,
CC ECO:0000269|PubMed:24443495}.
CC -!- SUBUNIT: Part of the multisubunit TRAPP (transport protein particle) II
CC complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65,
CC TRS85, TRS120 and TRS130. {ECO:0000303|PubMed:20713617}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network
CC {ECO:0000269|PubMed:24443495}. Early endosome
CC {ECO:0000269|PubMed:24443495}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems and flowers.
CC {ECO:0000269|PubMed:24443495}.
CC -!- DISRUPTION PHENOTYPE: Cytokinesis-defective and seedling-lethal
CC phenotype. {ECO:0000269|PubMed:20713617}.
CC -!- SIMILARITY: Belongs to the TRS120 family. {ECO:0000305}.
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DR EMBL; AL391222; CAC03452.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91628.1; -; Genomic_DNA.
DR EMBL; BT008594; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T51793; T51793.
DR RefSeq; NP_196665.1; NM_121142.3.
DR AlphaFoldDB; Q9FY61; -.
DR STRING; 3702.AT5G11040.1; -.
DR iPTMnet; Q9FY61; -.
DR PaxDb; Q9FY61; -.
DR PRIDE; Q9FY61; -.
DR ProteomicsDB; 234339; -.
DR EnsemblPlants; AT5G11040.1; AT5G11040.1; AT5G11040.
DR GeneID; 830971; -.
DR Gramene; AT5G11040.1; AT5G11040.1; AT5G11040.
DR KEGG; ath:AT5G11040; -.
DR Araport; AT5G11040; -.
DR TAIR; locus:2184901; AT5G11040.
DR eggNOG; KOG1953; Eukaryota.
DR HOGENOM; CLU_001678_0_0_1; -.
DR InParanoid; Q9FY61; -.
DR OMA; NYKKSQD; -.
DR OrthoDB; 71855at2759; -.
DR PhylomeDB; Q9FY61; -.
DR PRO; PR:Q9FY61; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FY61; baseline and differential.
DR Genevisible; Q9FY61; AT.
DR GO; GO:0005769; C:early endosome; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
DR GO; GO:0000919; P:cell plate assembly; IMP:TAIR.
DR GO; GO:0000911; P:cytokinesis by cell plate formation; IMP:TAIR.
DR InterPro; IPR013935; TRAPP_II_complex_Trs120.
DR PANTHER; PTHR21512; PTHR21512; 1.
DR Pfam; PF08626; TRAPPC9-Trs120; 3.
PE 1: Evidence at protein level;
KW Endosome; Golgi apparatus; Phosphoprotein; Reference proteome; Transport.
FT CHAIN 1..1186
FT /note="Trafficking protein particle complex II-specific
FT subunit 120 homolog"
FT /id="PRO_0000431448"
FT REGION 777..824
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 964..984
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 777..800
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 967..984
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 971
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
SQ SEQUENCE 1186 AA; 129595 MW; 1163643DFE18231F CRC64;
MEPDVSIETL SIIRIAVLPI GTIPPTLLRD YHSMLLRHHT IALSAISSFY TEHQKSPFTN
QPWDSGSLRF KFVLGGSPPS PWEDFQSNRK MLAVIGLCHC PSSPDLDSVT EKFNVACKSY
SSALVRRCFA FSPGDSQLED GDKKGENLIL FPPSDKQTQE FHLQTMMQDI AASLLMEFEK
WVLQAESAGT ILKTPLDSQA SLNSEEVIKA KKRRLGRAQK TIGDYSLLAG SPVDANAHYS
TALELARLTG DYFWYAGALE GSVCALLVDR MGQRDVALED EVRYRYTNVI LHYRKSFIQE
IAQRVSPLSF ELEATLKLAR FLCRRELAKE VVELLTNAAD GAKSLIDASD RLILYVEVAR
LFGALGYQRK AAFFCRQVAQ LYLQQDNRLA AISAMQVLSM TTDAYRIQSR ASMSKVSVNN
ETGRLPDAGK MHHHSIVSLF ESHWSTLQMV VLREILLSAV RAGDPLAAWS AAARLLRWHY
PLITPSGQNG LANSLANSAD RLPSGTRCAD PALPFVRLFS FPLHSSQVDI VKRNPAREDW
WTGSAPSGPF IYTPFSKGDA NESSKQELIW VVGEPVQVLV ELANPCCFDL RIDSIYLSAH
SSNFDAFPVS VDIPPNSAKV ITLSGIPTAV GPVTIPGCTV HCFGVITEHV FRDVDNLLLG
AAQGLVFSDP FRSCGSAKLR HVFVPNISVA PPLPLLVANV VGGDGAIILY EGEIREVCIN
FANAGTVPIV QAHVSLSGKN QDAVISIADE ALQSALPLKP GAQVTLPVTL KAWHVGPTDS
DNTMSSGRNA AGNTGRPKDG TSPSLLIHYA GPLSNNGDSQ EKESIVPPGR RLVVPLQICV
LQGLSFVKAR LLSMEIPAHV SDNLRDEDIE RESNADSLVK INPFRGSWGL RFLELELSNP
TDVVFEISVF VQLENSAKED DSSPVQDSPE YEYPKTRIDR DYSARVLIPL EHFKLPVLDG
SFFTKDPPPG SPSSSRNPSF SEKNTKAEIN TLIKNLISKI KVRWQSGRNS SGELDIKDAI
QTALQTTVMD VLLPDPLTFG FRLVRNGLEK DPETKAESPF SKGSVLSHEV TPMEVLVRNN
TSEAIKLNLS VTCRDVAGQN CTEGADATVL WAGALSGISM EVAPLQEARH CFSLFFLVPG
EYTMVAAAVI EDANNVLRAR AGTASPNEPI FCRGPPFHVC VAGGAL
