ID   TR135_RAT               Reviewed;         321 AA.
AC   Q67ES2;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Taste receptor type 2 member 135;
DE            Short=T2R135;
DE            Short=T2R35;
DE   AltName: Full=Taste receptor type 2 member 28;
DE            Short=T2R28;
GN   Name=Tas2r135 {ECO:0000250|UniProtKB:Q7TQA9}; Synonyms=Tas2r28;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1] {ECO:0000312|EMBL:AAR13357.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Sprague-Dawley {ECO:0000312|EMBL:AAR13357.1};
RA   Conte C., Ebeling M., Marcuz A., Andres-Barquin P.J.;
RT   "Identification of new putative rat taste receptors belonging to the T2R
RT   family.";
RL   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Putative taste receptor which may play a role in the
CC       perception of bitterness. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: Several bitter taste receptors are expressed in a single
CC       taste receptor cell. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC       {ECO:0000255}.
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DR   EMBL; AY362748; AAR13357.1; -; Genomic_DNA.
DR   RefSeq; NP_001020233.1; NM_001025062.1.
DR   AlphaFoldDB; Q67ES2; -.
DR   SMR; Q67ES2; -.
DR   STRING; 10116.ENSRNOP00000030941; -.
DR   GlyGen; Q67ES2; 1 site.
DR   iPTMnet; Q67ES2; -.
DR   PhosphoSitePlus; Q67ES2; -.
DR   PaxDb; Q67ES2; -.
DR   GeneID; 502757; -.
DR   KEGG; rno:502757; -.
DR   UCSC; RGD:1561078; rat.
DR   CTD; 387512; -.
DR   RGD; 1561078; Tas2r135.
DR   eggNOG; ENOG502S2SI; Eukaryota.
DR   InParanoid; Q67ES2; -.
DR   OrthoDB; 1010573at2759; -.
DR   PhylomeDB; Q67ES2; -.
DR   Reactome; R-RNO-418594; G alpha (i) signalling events.
DR   Reactome; R-RNO-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR   PRO; PR:Q67ES2; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; ISO:RGD.
DR   GO; GO:0033038; F:bitter taste receptor activity; IBA:GO_Central.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR   GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IBA:GO_Central.
DR   InterPro; IPR007960; TAS2R.
DR   Pfam; PF05296; TAS2R; 1.
PE   3: Inferred from homology;
KW   G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW   Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..321
FT                   /note="Taste receptor type 2 member 135"
FT                   /id="PRO_0000247662"
FT   TOPO_DOM        1..28
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        29..49
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        50..75
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        76..96
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        97..106
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        107..127
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        128..149
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        171..207
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        208..228
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        229..253
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        275..286
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        287..307
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        308..321
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        188
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   321 AA;  36491 MW;  8ECCC9B2C72F1199 CRC64;
     MGPIMSTGET STAHTVLGCQ ITDKTVITLF VILVFSCLVA VVGNGFIIIA LGMKWLLRRT
     LSAHNKLLIS LAASRFCLQC VVIGKNIYVF LNPSSFPYNP VIQLLNLMWD FLTAATIWFC
     SLLGFFYCVK IATLTHPVFV WLKYRLPGWV PWMLLSAVGM SSLTSILCFI GNHMIYQNYA
     RRGHQPWNAT GNSLRHSLEK FYFISIKIIM WTVPTVIFSI FMSLLLVSLV RHMKKTLLAL
     SELRDVWAQA HFKALLPLLS FIILFISCFL TLVLSSASST PYQEFRYWMW QVVIHLCTVI
     HPIVILLSNP VLRVVMKRGC C