TR140_MOUSE
ID TR140_MOUSE Reviewed; 312 AA.
AC Q7TQA4; A6H6Q9; Q9JKA0; Q9JKA2;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Taste receptor type 2 member 140;
DE Short=T2R140;
DE AltName: Full=T2R13;
DE AltName: Full=T2R8;
DE AltName: Full=Taste receptor family B member 3;
DE Short=mTRB3;
DE Short=mTRB5;
DE AltName: Full=Taste receptor type 2 member 40;
DE Short=T2R40;
DE AltName: Full=mT2r64;
GN Name=Tas2r140; Synonyms=T2r64, Tas2r13, Tas2r137, Tas2r40, Tas2r8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|EMBL:AAP40341.1};
RN [1] {ECO:0000312|EMBL:AAP40341.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAP40341.1};
RX PubMed=12734386; DOI=10.1152/physiolgenomics.00060.2003;
RA Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J.;
RT "Evolutionary relationships of the Tas2r receptor gene families in mouse
RT and human.";
RL Physiol. Genomics 14:73-82(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 32-277.
RC STRAIN=C57BL/6J, and DBA/2J;
RX PubMed=10766242; DOI=10.1038/35007072;
RA Matsunami H., Montmayeur J.-P., Buck L.B.;
RT "A family of candidate taste receptors in human and mouse.";
RL Nature 404:601-604(2000).
RN [5] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=12679530; DOI=10.1093/molbev/msg083;
RA Shi P., Zhang J., Yang H., Zhang Y.-P.;
RT "Adaptive diversification of bitter taste receptor genes in mammalian
RT evolution.";
RL Mol. Biol. Evol. 20:805-814(2003).
RN [6]
RP REVIEW.
RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [7]
RP REVIEW.
RX PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [8]
RP REVIEW.
RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
CC -!- FUNCTION: Receptor that may play a role in the perception of bitterness
CC and is gustducin-linked. May play a role in sensing the chemical
CC composition of the gastrointestinal content. The activity of this
CC receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation
CC and lead to the gating of TRPM5.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC tongue and palate epithelium and exclusively in gustducin-positive
CC cells. Expressed in 15% taste bud cells in circumvallate and foliate
CC papillae but only in 2% in fungiform papillae.
CC -!- MISCELLANEOUS: Most taste cells may be activated by a limited number of
CC bitter compounds; individual taste cells can discriminate among bitter
CC stimuli. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000305}.
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DR EMBL; AF532791; AAP40341.1; -; Genomic_DNA.
DR EMBL; AC151055; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC145965; AAI45966.1; -; mRNA.
DR EMBL; BC145967; AAI45968.1; -; mRNA.
DR EMBL; AF247733; AAF64510.1; -; Genomic_DNA.
DR EMBL; AF247735; AAF64512.1; -; mRNA.
DR EMBL; BK001092; DAA01231.1; -; Genomic_DNA.
DR CCDS; CCDS20633.1; -.
DR RefSeq; NP_067537.1; NM_021562.1.
DR AlphaFoldDB; Q7TQA4; -.
DR SMR; Q7TQA4; -.
DR STRING; 10090.ENSMUSP00000093040; -.
DR GlyGen; Q7TQA4; 3 sites.
DR PaxDb; Q7TQA4; -.
DR PRIDE; Q7TQA4; -.
DR Ensembl; ENSMUST00000095391; ENSMUSP00000093040; ENSMUSG00000071147.
DR GeneID; 387616; -.
DR KEGG; mmu:387616; -.
DR UCSC; uc009ejy.1; mouse.
DR CTD; 387616; -.
DR MGI; MGI:2681298; Tas2r140.
DR VEuPathDB; HostDB:ENSMUSG00000071147; -.
DR eggNOG; ENOG502SKRK; Eukaryota.
DR GeneTree; ENSGT00960000186648; -.
DR HOGENOM; CLU_072337_3_0_1; -.
DR InParanoid; Q7TQA4; -.
DR OMA; IHINASI; -.
DR OrthoDB; 1053374at2759; -.
DR PhylomeDB; Q7TQA4; -.
DR TreeFam; TF335891; -.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR Reactome; R-MMU-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-MMU-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR BioGRID-ORCS; 387616; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q7TQA4; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q7TQA4; protein.
DR Bgee; ENSMUSG00000071147; Expressed in spermatid.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0033038; F:bitter taste receptor activity; ISO:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0008527; F:taste receptor activity; ISO:MGI.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; ISO:MGI.
DR InterPro; IPR007960; TAS2R.
DR Pfam; PF05296; TAS2R; 1.
PE 2: Evidence at transcript level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..312
FT /note="Taste receptor type 2 member 140"
FT /id="PRO_0000082293"
FT TOPO_DOM 1..9
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..90
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..184
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..229
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..264
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..312
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 2
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 86
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 162
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 32
FT /note="I -> G (in Ref. 4; AAF64512)"
FT /evidence="ECO:0000305"
FT CONFLICT 68
FT /note="T -> M (in Ref. 4; AAF64510)"
FT /evidence="ECO:0000305"
FT CONFLICT 110
FT /note="M -> K (in Ref. 4; AAF64510)"
FT /evidence="ECO:0000305"
FT CONFLICT 135
FT /note="L -> M (in Ref. 4; AAF64510)"
FT /evidence="ECO:0000305"
FT CONFLICT 173
FT /note="I -> L (in Ref. 4; AAF64510)"
FT /evidence="ECO:0000305"
FT CONFLICT 197
FT /note="I -> T (in Ref. 4; AAF64510)"
FT /evidence="ECO:0000305"
FT CONFLICT 263
FT /note="F -> L (in Ref. 4; AAF64510)"
FT /evidence="ECO:0000305"
FT CONFLICT 265
FT /note="Y -> F (in Ref. 4; AAF64510)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 312 AA; 35555 MW; CCECA4E0EB4FF8B2 CRC64;
MNATVKCTLL IILGVEIIIG CLGNGFIAVV NIMDWAKRRK ISLVDQIFTA LAISRLAFVW
SLLTVLFTSE LYSALMTTRK VLIIFNNSWT VINHFNIWLA TCLSIFYFLM IANFSNSIFL
SLRWRVKTVV SVTLLMSLLL LFVNVLVINT FIVISVDVYK VNTSYSSHSD NNIHISRIFL
FTNTIFTFIP FSVTLTIFLL LIFSLWTHLK NMQHNAKDSR DPSTTAHIKA LQMMVTFLLL
YTIFFLALVM QSSKMKFLSS TVFNYFFEVI SLAFPSGHSC VLILGNSKLR QTFISTVWWL
KSSFNAAELP GP
