ID   TR143_RAT               Reviewed;         294 AA.
AC   Q67ES3;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Taste receptor type 2 member 143;
DE            Short=T2R143;
DE   AltName: Full=Taste receptor type 2 member 27;
DE            Short=T2R27;
GN   Name=Tas2r143 {ECO:0000250|UniProtKB:Q7TQB9}; Synonyms=Tas2r27;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1] {ECO:0000312|EMBL:AAR13356.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Sprague-Dawley {ECO:0000312|EMBL:AAR13356.1};
RA   Conte C., Ebeling M., Marcuz A., Andres-Barquin P.J.;
RT   "Identification of new putative rat taste receptors belonging to the T2R
RT   family.";
RL   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Putative taste receptor which may play a role in the
CC       perception of bitterness. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: Several bitter taste receptors are expressed in a single
CC       taste receptor cell. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC       {ECO:0000255}.
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DR   EMBL; AY362747; AAR13356.1; -; Genomic_DNA.
DR   RefSeq; NP_001020232.1; NM_001025061.1.
DR   RefSeq; XP_008761149.1; XM_008762927.2.
DR   RefSeq; XP_008773926.1; XM_008775704.2.
DR   AlphaFoldDB; Q67ES3; -.
DR   SMR; Q67ES3; -.
DR   STRING; 10116.ENSRNOP00000036134; -.
DR   GlyGen; Q67ES3; 1 site.
DR   PaxDb; Q67ES3; -.
DR   PRIDE; Q67ES3; -.
DR   Ensembl; ENSRNOT00000085902; ENSRNOP00000073280; ENSRNOG00000053213.
DR   GeneID; 502756; -.
DR   KEGG; rno:502756; -.
DR   UCSC; RGD:1566290; rat.
DR   CTD; 387514; -.
DR   RGD; 1566290; Tas2r143.
DR   eggNOG; ENOG502S2SI; Eukaryota.
DR   GeneTree; ENSGT00960000186648; -.
DR   HOGENOM; CLU_072337_1_1_1; -.
DR   InParanoid; Q67ES3; -.
DR   OMA; ALWEFIN; -.
DR   OrthoDB; 1282504at2759; -.
DR   PhylomeDB; Q67ES3; -.
DR   TreeFam; TF335891; -.
DR   PRO; PR:Q67ES3; -.
DR   Proteomes; UP000002494; Chromosome 4.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0033038; F:bitter taste receptor activity; IBA:GO_Central.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR   GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IBA:GO_Central.
DR   InterPro; IPR007960; TAS2R.
DR   Pfam; PF05296; TAS2R; 1.
PE   3: Inferred from homology;
KW   G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW   Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..294
FT                   /note="Taste receptor type 2 member 143"
FT                   /id="PRO_0000247665"
FT   TOPO_DOM        1..7
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        8..28
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        29..43
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..64
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        65..80
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..101
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        102..128
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        129..149
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        150..180
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        181..201
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        202..227
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        228..248
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        249..260
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        261..281
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        282..294
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        162
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   294 AA;  34111 MW;  F7F77A04ADCF38C5 CRC64;
     MPSTPTLIFI VIFFLVSVAS MLQNGFMIIV LGREWMRNRA LPAVDMIVAS LASSRFCLHG
     IAILNNFLAS FDFCYQANFV GILWDFINTL ILWLTAWLAI FYCVKISSFS HPVLFWLKWR
     ISQLVPRLLL VSLIMGGLSA IISATGNIIA NQMIISQGFH GNCTFGHMSL DFYRYYYLSH
     AVLMWFTPFF LFLVSIIFLM FSLYRHVEKM RGHRPGPWDP RTQAHTMALK SLTVFITFYI
     LFFLALIISS TKSKTMHSYW YWVREIIIYT GIFLNSIILV LSNPKLRKAL KMRF