ID   TR2M_AGRRH              Reviewed;         749 AA.
AC   Q09109; Q841R5;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Tryptophan 2-monooxygenase;
DE            EC=1.13.12.3;
GN   Name=aux1;
OS   Agrobacterium rhizogenes.
OG   Plasmid pRiA4.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A4;
RX   PubMed=1932811; DOI=10.1094/mpmi-4-155;
RA   Camilleri C., Jouanin L.;
RT   "The TR-DNA region carrying the auxin synthesis genes of the Agrobacterium
RT   rhizogenes agropine-type plasmid pRiA4: nucleotide sequence analysis and
RT   introduction into tobacco plants.";
RL   Mol. Plant Microbe Interact. 4:155-162(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
RC   STRAIN=A4;
RX   PubMed=8510649; DOI=10.1007/bf00281622;
RA   Gaudin V., Camilleri C., Jouanin L.;
RT   "Multiple regions of a divergent promoter control the expression of the
RT   Agrobacterium rhizogenes aux1 and aux2 plant oncogenes.";
RL   Mol. Gen. Genet. 239:225-234(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-tryptophan + O2 = CO2 + H2O + indole-3-acetamide;
CC         Xref=Rhea:RHEA:16165, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16031, ChEBI:CHEBI:16526, ChEBI:CHEBI:57912;
CC         EC=1.13.12.3;
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC       Note=Binds 1 FMN per subunit. {ECO:0000250};
CC   -!- PATHWAY: Plant hormone metabolism; auxin biosynthesis.
CC   -!- SIMILARITY: Belongs to the tryptophan 2-monooxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; M61151; AAA22080.1; -; Genomic_DNA.
DR   EMBL; S62276; AAP13934.1; -; Genomic_DNA.
DR   PIR; I39708; I39708.
DR   AlphaFoldDB; Q09109; -.
DR   SMR; Q09109; -.
DR   STRING; 359.CN09_18940; -.
DR   eggNOG; COG1231; Bacteria.
DR   UniPathway; UPA00151; -.
DR   GO; GO:0050361; F:tryptophan 2-monooxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009851; P:auxin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR006064; Glycosidase.
DR   InterPro; IPR012142; Trp_2-mOase.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   Pfam; PF02027; RolB_RolC; 1.
DR   PIRSF; PIRSF000319; Trp_2-mono_O2ase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   3: Inferred from homology;
KW   Auxin biosynthesis; Flavoprotein; FMN; Monooxygenase; Oxidoreductase;
KW   Plasmid.
FT   CHAIN           1..749
FT                   /note="Tryptophan 2-monooxygenase"
FT                   /id="PRO_0000065585"
FT   BINDING         232
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         252
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         260
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         280
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         280
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   749 AA;  83178 MW;  8B7793970206F97B CRC64;
     MAGSSFTLPS TGSAPLDMML IDDSDLLQLG LQQVFSKRYT ETPQSRYKLT RRASPDVSSG
     EGNVHALAFI YVNAETLQMI KNARSLTEAN GVKDLVAIDV PPFRNDFSRA LLLQVINLLG
     NNRNADDDLS HFIAVALPNS ARSKILTTAP FEGSLSENFR GFPITREGNV ACEVLAYGNN
     LMPKACSDSF PTVDLLYDYG KFFESCAADG RIGYFPEGVT KPKVAIIGAG FSGLVAASEL
     LHAGVDDVTV YEASDRLGGK LWSHGFKSAP NVIAEMGAMR FPRSESCLFF YLKKHGLDSV
     GLFPNPGSVD TALFYRGRQY IWKAGEEPPE LFRRVHHGWR AFLQDGYLHD GVMLASPLAI
     VDALNLGHLQ QAHGFWQSWL TYFERESFSS GIEKMFLGNH PPGGEQWNSL DDLDLFKALG
     IGSGGFGPVF ESGFIEILRL VVNGYEDNVR LSYEGISELP HRIASQVING RSIRERTIHV
     QVEQIDREED KINIKIKGGK VEVYDRVLVT SGFANIEMRH LLTSSNAFFH ADVSHAIGNS
     HMTGASKLFL LTNEKFWLQH HLPSCILTTG VAKAVYCLDY DPRDPSGKGL VLISYTWEDD
     SHKLLAVPDK RERFASLQRD IGRAFPDFAK HLTPADGNYD DNIVQHDWLT DPHAGGAFKL
     NRRGNDVYSE RLFFQPFDVM HPADDKGLYL AGCSCSFTGG WVHGAIQTAC NATCAIIYGS
     GHLQELIHWR HLKEGNPLAH AWKRYRYQA