ID   TR2M_RHIRD              Reviewed;         755 AA.
AC   P0A3V3; P04029;
DT   23-OCT-1986, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=Tryptophan 2-monooxygenase;
DE            EC=1.13.12.3;
GN   Name=tms1;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OG   Plasmid pTiA6NC.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6584906; DOI=10.1073/pnas.81.6.1728;
RA   Klee H., Montoya A., Horodyski F., Lichtenstein C., Garfinkel D.,
RA   Fuller S., Flores C., Peschon J., Nester E., Gordon M.;
RT   "Nucleotide sequence of the tms genes of the pTiA6NC octopine Ti plasmid:
RT   two gene products involved in plant tumorigenesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:1728-1732(1984).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-tryptophan + O2 = CO2 + H2O + indole-3-acetamide;
CC         Xref=Rhea:RHEA:16165, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16031, ChEBI:CHEBI:16526, ChEBI:CHEBI:57912;
CC         EC=1.13.12.3;
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC       Note=Binds 1 FMN per subunit. {ECO:0000250};
CC   -!- PATHWAY: Plant hormone metabolism; auxin biosynthesis.
CC   -!- MISCELLANEOUS: The sequence shown is that of Ach5.
CC   -!- SIMILARITY: Belongs to the tryptophan 2-monooxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; K02554; AAA92550.1; -; Genomic_DNA.
DR   PIR; A04497; QQAG4T.
DR   AlphaFoldDB; P0A3V3; -.
DR   SMR; P0A3V3; -.
DR   BioCyc; MetaCyc:MON-7863; -.
DR   UniPathway; UPA00151; -.
DR   GO; GO:0050361; F:tryptophan 2-monooxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009851; P:auxin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR006064; Glycosidase.
DR   InterPro; IPR012142; Trp_2-mOase.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   Pfam; PF02027; RolB_RolC; 1.
DR   PIRSF; PIRSF000319; Trp_2-mono_O2ase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   3: Inferred from homology;
KW   Auxin biosynthesis; Crown gall tumor; Flavoprotein; FMN; Monooxygenase;
KW   Oxidoreductase; Plasmid.
FT   CHAIN           1..755
FT                   /note="Tryptophan 2-monooxygenase"
FT                   /id="PRO_0000065587"
FT   BINDING         247
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         267
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         275
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         295
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         295
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   755 AA;  83892 MW;  84D2A325594964D8 CRC64;
     MSASPLLDNQ CDHLPTKMVD LTMVDKADEL DRRVSDAFLE REASRGRRIT QISTECSAGL
     ACKRLADGRF PEISAGGKVA VLSAYIYIGK EILGRILESK PWARATVSGL VAIDLAPFCM
     DFSEAQLIQA LFLLSGKRCA PIDLSHFVAI SISKTAGFRT LPMPLYENGT MKCVTGFTIT
     LEGAVPFDMV AYGRNLMLKG SAGSFPTIDL LYDYRPFFDQ CSDSGRIGFF PEDVPKPKVA
     VIGAGISGLV VANELLHAGV DDVTIYEASD RVGGKLWSHA FRDAPSVVAE MGAMRFPPAA
     FCLFFFLERY GLSSMRPFPN PGTVDTYLVY QGVQYMWKAG QLPPKLFHRV YNGWRAFLKD
     GFYERDIVLA SPVAITQALK SGDIRWAHDS WQIWLNRFGR ESFSSGIERI FLGTHPPGGE
     TWSFPHDWDL FKLMGIGSGG FGPVFESGFI EILRLVINGY EENQRMCPEG ISELPRRIAS
     EVVNGVSVSQ RICHVQVRAI QKEKTKIKIR LKSGISELYD KVVVTSGLAN IQLRHCLTCD
     TNIFQAPVNQ AVDNSHMTGS SKLFLMTERK FWLDHILPSC VLMDGIAKAV YCLDYEPQDP
     NGKGLVLISY TWEDDSHKLL AVPDKKERLC LLRDAISRSF PAFAQHLFPA CADYDQNVIQ
     HDWLTDENAG GAFKLNRRGE DFYSEELFFQ ALDTANDTGV YLAGCSCSFT GGWVEGAIQT
     ACNAVCAIIH NCGGILAKGN PLEHSWKRYN YRTRN