TR732_YEAST
ID TR732_YEAST Reviewed; 1420 AA.
AC Q03496; D6W085; Q04862;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=tRNA (cytidine(32)-2'-O)-methyltransferase non-catalytic subunit TRM732;
GN Name=TRM732; OrderedLocusNames=YMR259C; ORFNames=YM8156.01C, YM9920.13C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, AND INTERACTION WITH TRM7.
RX PubMed=22912484; DOI=10.1261/rna.035287.112;
RA Guy M.P., Podyma B.M., Preston M.A., Shaheen H.H., Krivos K.L.,
RA Limbach P.A., Hopper A.K., Phizicky E.M.;
RT "Yeast Trm7 interacts with distinct proteins for critical modifications of
RT the tRNAPhe anticodon loop.";
RL RNA 18:1921-1933(2012).
CC -!- FUNCTION: Required together with TRM7 for the methylation of the 2'-O-
CC ribose of cytidine at position 32 of the tRNA anticodon loop of
CC tRNA(Phe) and tRNA(Leu(UAA)). {ECO:0000269|PubMed:22912484}.
CC -!- SUBUNIT: Interacts with TRM7; for 2'-O-methylation of cytidine 32 in
CC substrate tRNAs. {ECO:0000269|PubMed:22912484}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 7110 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z49260; CAA89242.1; -; Genomic_DNA.
DR EMBL; Z48639; CAA88586.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10159.1; -; Genomic_DNA.
DR PIR; S54471; S54471.
DR RefSeq; NP_013986.1; NM_001182766.1.
DR AlphaFoldDB; Q03496; -.
DR BioGRID; 35437; 32.
DR MINT; Q03496; -.
DR STRING; 4932.YMR259C; -.
DR iPTMnet; Q03496; -.
DR MaxQB; Q03496; -.
DR PaxDb; Q03496; -.
DR PRIDE; Q03496; -.
DR EnsemblFungi; YMR259C_mRNA; YMR259C; YMR259C.
DR GeneID; 855301; -.
DR KEGG; sce:YMR259C; -.
DR SGD; S000004872; TRM732.
DR VEuPathDB; FungiDB:YMR259C; -.
DR eggNOG; KOG1810; Eukaryota.
DR GeneTree; ENSGT00390000015500; -.
DR HOGENOM; CLU_001011_2_0_1; -.
DR OMA; TQHITRR; -.
DR BioCyc; YEAST:G3O-32934-MON; -.
DR PRO; PR:Q03496; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q03496; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR GO; GO:0002130; P:wobble position ribose methylation; IMP:SGD.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR019442; THADA/TRM732_DUF2428.
DR Pfam; PF10350; DUF2428; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome; tRNA processing.
FT CHAIN 1..1420
FT /note="tRNA (cytidine(32)-2'-O)-methyltransferase non-
FT catalytic subunit TRM732"
FT /id="PRO_0000203343"
SQ SEQUENCE 1420 AA; 163577 MW; AFBB0CF83CBB169D CRC64;
MTTDVQFSSQ EIELFRVKEF LIANNPAKIN NENKDAVLTQ IEHDFRYLIQ YIKDGLPNLN
ESTRLIFPDT FSICLLRSHQ IIASKKIDSQ EFLSAVKEQL LTEANANIIF EYVLDFWADG
GAPLMNALRD LFSKLLNLLK ITYPMSTLKD VLFNWMNEIL EVPSTLRVQY YLIDALSSDF
DLYYIIEKKP HFIDNSFSLM NSDLLANSVG KCIVSLLLNI YEKHFKKNES FVQEWIQLWK
SCALKYIHDK QYTKSINLYI MIPLFKNMPN SAFTLFLECM SNKDPSLLLS LLKIGQELGI
EEEPFCDNKY TTVDSVNKLI EQDEYKLQVF EILTFSTKKS KPIRPFVFKT IKQYLYVFFV
DTELERRNYF CSSMKHFIFR TRDCAYSLAR DARKLKKAEK FPDEQREKLA QVEEARAFLV
WLCNFIKYNL APGTLYQANV TSLKLMHILI KSGVDKSTPQ KFLDNQNKRE YPFSIPILQD
VTFLRLLIDL LVSNYADVRE LSKEMLFIMI SADESRGLFL DTLDANALKW TATSLLSDYE
KGDAGATVYE FIFTVMGSQR SFIDQTIDIL AQMVQNLQND SIGCAENSIG PHFAALSLIL
NKFNSEENHQ DTSKIISKLI NLVLKSWEAT RNVVCHDSAH GILPEKYANC GVPDQVIISH
AFRAIKEASY LLETILKKYP LTRDQLDSIG DLFIVQLSTI RHSGAFQAVL PGLKAFCIRC
QLEFPAILEE LLSKSVKSLK SKTQHITRRS GGLPFLVTTV LSAEVTKGRP LLQKTFENLL
LVARLPIPPH QDEFDLPQVN AINCINAIFV EPKLSVHCTS FVSEALELAL LNFDCDIWAL
RNCSIMLFTS LQNRIFGKVG RSVSAKLFFT KYSGLRQLLL NILNSSIAQY SGSERKSYQI
ESIFLVLNVL LRLRPTAGYT GLKEFNVSVY ECLSNENWKI RDMASRVLHM LSENFEEEIR
KLLDLASIAK QNQLHGHLLA LQQLVPQYLS GTRDMELIQR ILEKKRMLLL ENKCFITKKA
YLKLTCCILE TCDIPDSILK DYISTLRNTF IAENNEYVVD GSKQLYLAQI LDMLLKYEDS
IYLDDICLLG LYSPFYEAQL STLQYMNTNF HWETTRNSEF LEQLQLLLRV PDLLPMAKAL
VVKILSRKKN TLSLTTCTDL LKTNNSEDTK LAAVSSLSAK LSSQTFHQVW NLLQGFFADS
CSKDFRLASL ECLTAYPESC KNSRILLQLY NFLWDDDSEI REKASFYLNK NFIQTADWEY
NRNTSVTALI FTKKFVDVFT SSEVVEELCL QLFQYLNEYD MFAAEESAKN CLFTIEKDNQ
FINELQKAMH ILNMIKLTGR DISKCYKDQI HHLKSALLEH FNTEDFKDSP LGWCSNAEIF
SRITLLKELI QHYSPSDYEN FINVLTKHSV HPLIISYSQL
