TRA1_SCHPO
ID TRA1_SCHPO Reviewed; 3699 AA.
AC Q9HFE8;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Transcription-associated protein 1;
GN Name=tra1; ORFNames=SPBP16F5.03c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Essential component of histone acetyltransferase (HAT)
CC complexes, which serves as a target for activators during recruitment
CC of HAT complexes. Essential for vegetative growth. Functions as a
CC component of the transcription regulatory histone acetylation (HAT)
CC complexes SAGA, SALSA and SLIK. At the promoters, SAGA is required for
CC recruitment of the basal transcription machinery. It influences RNA
CC polymerase II transcriptional activity through different activities
CC such as TBP interaction and promoter selectivity, interaction with
CC transcription activators, and chromatin modification through histone
CC acetylation and deubiquitination. SAGA acetylates nucleosomal histone
CC H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA
CC interacts with DNA via upstream activating sequences (UASs). SALSA, an
CC altered form of SAGA, may be involved in positive transcriptional
CC regulation (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the SAGA complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Although strongly related to the PI3/PI4-kinase family,
CC it lacks the typical motifs that constitute the catalytic site of
CC PI3/PI4-kinase proteins, suggesting that it may lack such activity.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. TRA1 subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CU329671; CAC08542.1; -; Genomic_DNA.
DR RefSeq; NP_595777.1; NM_001021677.2.
DR SMR; Q9HFE8; -.
DR BioGRID; 277779; 38.
DR IntAct; Q9HFE8; 24.
DR MINT; Q9HFE8; -.
DR STRING; 4896.SPBP16F5.03c.1; -.
DR iPTMnet; Q9HFE8; -.
DR MaxQB; Q9HFE8; -.
DR PaxDb; Q9HFE8; -.
DR EnsemblFungi; SPBP16F5.03c.1; SPBP16F5.03c.1:pep; SPBP16F5.03c.
DR GeneID; 2541265; -.
DR KEGG; spo:SPBP16F5.03c; -.
DR PomBase; SPBP16F5.03c; tra1.
DR VEuPathDB; FungiDB:SPBP16F5.03c; -.
DR eggNOG; KOG0889; Eukaryota.
DR HOGENOM; CLU_000129_1_0_1; -.
DR InParanoid; Q9HFE8; -.
DR OMA; NPIFAMD; -.
DR PhylomeDB; Q9HFE8; -.
DR PRO; PR:Q9HFE8; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0070209; C:ASTRA complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000124; C:SAGA complex; IDA:PomBase.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR GO; GO:0016573; P:histone acetylation; IEA:InterPro.
DR GO; GO:0010674; P:negative regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle; IMP:PomBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:GOC.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; EXP:PomBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR InterPro; IPR033317; TRA1/TRRAP.
DR PANTHER; PTHR11139:SF1; PTHR11139:SF1; 2.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF48371; SSF48371; 3.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 2.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW Activator; Chromatin regulator; Nucleus; Reference proteome; Repeat;
KW TPR repeat; Transcription; Transcription regulation.
FT CHAIN 1..3699
FT /note="Transcription-associated protein 1"
FT /id="PRO_0000314773"
FT REPEAT 314..347
FT /note="TPR 1"
FT REPEAT 2082..2114
FT /note="TPR 2"
FT DOMAIN 2546..3107
FT /note="FAT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00534"
FT REPEAT 2610..2643
FT /note="TPR 3"
FT DOMAIN 3333..3669
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT DOMAIN 3667..3699
FT /note="FATC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00534,
FT ECO:0000255|PROSITE-ProRule:PRU00535"
FT REGION 197..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3339..3345
FT /note="G-loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 3535..3543
FT /note="Catalytic loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 3555..3580
FT /note="Activation loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT COMPBIAS 197..215
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3699 AA; 422339 MW; 1B5810C719C89BAB CRC64;
MQLALSHNIP IVIVKYLGKR IRVQLEFKKE LAQRSKHLMD VSQCEHWHNR LCDVGLDSKQ
KANTAIEIRD ALDDVLVTEK TNFETFIPLL EDTLSLLEKE RPVFSSLAAT HRLRIALLEL
LKKSGSYKGF EAFVNRTFAV LLRIVVNDNE EMAVLALKLV VLLFKDHSSL AKGHVQEFLS
IVVENYKSMT TVVSEAFPPR SAPNTPSSHP MSAASSASPA EIGMEHAGPK MIPKASSSFK
VTAEFPIIVF LLFQTYKDLI PKMLPLLAPL VLQFISLRPP PQAEARRLAE SQKEVFIGVV
PSLRRNHLYN DLISAQIKSF SFLAYLLRSF GAALKQFESS IPICTLQLFM DCPSELYQTR
RELLVATRHV LSTDYLRGFL PYVDQLLDTK ILVGSGITSQ HSLRPMAFSM LADMLHYVRM
ELSPQQIYKV ILLYFSILMD DFYTSAIQAM ATKLILNLVE RIVALEDFST SRSLLFAILL
CLLRKLTSLN FEFMKLRDSL QENADLKQIK IEENKHDLPM FENPTGAAQP SGLDKLKDCI
FLFKNTLLGI KPVLFGLKQR NIPLANGSIF TAQEWSEKLH LSSTNEVLLF RRLLVESLKG
FSYYQTDEKT GVFKSSKNLA YSQLDSSLTT NPSKLLEEKE LLEMLATLFL HLDPSVFVEI
LESEFPNIFE CLVDNLALLH IFQFWMSNEV TSVNCTGIVL SFCCDNLAKI GSGQSTRVSV
LLRLFKLAFM TVNVFPEKNA EVLRPHISYI ISTSLELTTD AVEPLNYVYL MKALFRNISG
GKFDSLYKEI LPLLQVMLEC FNRLIFTVTS TSQKELYAEL CLTLPIRLSV LLPHMNFLMK
PLIVALKGPP EIASQGLRIF ELCLDNLTQE FLDPLLDSIM PDLLICLWNH SRLNQSNNQL
HQSAVRILGK MGGRNRQIYL GTFGFDFLQD ENIFPSIQFS FQGSSQNFSL EHSKFLMSSC
AVLNNQNSDL EEKKQAFQMV KNSYLLLFAS AKPDEDFWES IDTMCRAVVD RMDKNLQQVS
NGRLCPDKDE SYYLQRSIVS NIFKSLVGSM SCVEFVAEAR ETINRSLEWL IVLDLVNYAD
SLQIKDQNIF DNLQSIKMLD LTTCINGIFE SLCSENENTR SNALSCIDHY LNAHKMLLNT
TLDISKLPSF QNLVTVFCQS CHKELWYQKN AGFLGLKAIL SYDSHHKLWI QDRLHDILKA
LFFILKDTPT DYGVLKLTEV RSFIVDITTQ FCILQDVLAP KERANNIINA FSPFFLELLH
PNDHVRNTVQ QAIENISNNS KLSVVDLLLP IKDRLLSPIF GKPLRALPFT IQIGHIDAIT
YCLHRSPSFL DLTDELYRLF RETIALADAE DEALVTMLKT SQSKDSSSLR KLRATCLHLL
FASLVAHKFD QPQHAQTRTK IIAIFFKDLY SPHKEIYSVA IDALRHVLSQ NQKLPKELLQ
SGLRPILMNL SDHNKLSVNG LEGLSRLLRL LTNYFKVEIG RKLLQHLNVL SDSKVLETAS
LSLLKTNPRI EIIVSLVNVF RDLPPLSAQF LGDLLSSVVN IEAVLRKYSN SPLRKPLYSF
MDLHANDTWM YILNNARNGD LITRFVGALN DPMSEKLRET AGNYWGKLLE LISQPVSVEN
LAPMYAVDII ATVFPYISAN VDAGVISAKF IVLSKSLYGM LSSYNEYLFL PIRRCISSIT
KMLLSSLKKI EKKLEFSLEV FRFKADDDND FLPEYIDSLC GCLITSTSAA EKKSIFLVCC
SIVGDKSVRP FFKAFLLDKV INPLVFKSCG ENNFIDKDVV HSVYTHVWRV SIRDFAEVSG
ATDSFYMGIM CLTTALCKYH SALLNDYRKS VIMSAWNYIK LEDPMVKQAA YATIACFISA
YDTPAKIVTP VYVSILKTYQ PEVRAFIEFS LASLLSVLTA RLSSPSDSTF PLWAKLPRLV
ISEDVQGISQ PLTVYQFICK APDLFFSCCS HFIVPMVNAL PKLVSFSSAS TEPRKLALDI
VQTFINWQRK QNESENSETT LFSNSHIEAI LTFLIKFLSL FPEPVEENPL SKKGLSLFND
LFSFPRWKDC QLNSNFFEKI LVDMDFNDNN YRTVANTLFV FGVILKNRGM EYIQREYSHI
IALIDKSLRC GKLPVTHSLE QIILLLLQSH PTQAEEEEDT NEADDFKQLL LSVIHDNLAA
ATNIESAICY LQIVKSSNPE ALDGLLLPLN KCFQKVARDH IVACMQSAIQ ASGKVTLPSA
SDTVSKLLIS FIEIIRVRMA SLGDQRRWFL SVVVQLLEKS SSFELCEHIL NVTKEWVIVK
RDSFLTVKEK TALLLKMRTF EGRFDNKLYI EACDLLSTIY RDPIFAHTEL TARLKQAFLL
ATASKDTKIR MDFMDIFDSS MSRNVYSRLT FILDATSWDT IPSIYWIKQA NYILLGAINA
KQPVRLTDNS LRFAPVPMTK PILSSLPEVF SKHNGSAIPL GRFTFFKQLD LFLKRNKELT
VQKIIFPLAH IQMLSDADAN KLWQYIFPLA WKILSSDNRS DLSKSLIYLL TRDYHIKQVN
NRPNVISTLV SSFVKCAAKL ELPPHLVKYL GKLYGVYHES VSLLEIQLSD KYDMYQNAKV
QESRADAVAE LYASLNEDDM FYGHWRRNCK YLQTQVALSY EQLGMWGRAQ QLYEQAQTKA
RSEAIPFSES EYNLWEDQWV MCAQKLQQWD VLTELAKHEG SSELLLECAW RISDWSNNRE
SLEVAIKSLS DVPTPRKLTF QCFMTLQKSV SQPLAIKEFQ QVLSEAIQLA LIKWHQLPEK
VNQSHYSLLH LFQQFVELQE AATIYSHLNA INFQNLPTNV QLIKSALQVW QERLPNVWDD
INLWRDLISW RQIVFSMINR VYLPLVPTIQ ANSSADSSNP PNTSFLFRGY HETAWLINRF
AHVARKHKLP SVCLNQLTKI YTLPNIEIQE AFYKLREQVL CYLQNPRDLK TGLEVVTNTN
LMYFNSRQKS EFVTLKGKFL EKLNRGEEAN QMYAAAVQID LGLPKAWAEW GRYNDLLFNK
SPDNLSAACN AISCYLQAAG TYQSSKARKM LARVLWLLSL NDSAGTIVKA FESYKGDIPV
WHWLTFIPQL LNSLSKGDTK CAPVVLKKIA KSYPQALFFT LRTAREDIAQ VKRTEMAAWK
SNTTDENKRN EDILSIQSNF LSTNQSTNAP ATNKEDGLSK KVWEYIDEIM SILKTAYPLL
ALTMETLVDQ IQAKFKCKND GDAFRLVVAL LNDAVQHSIR LGIVTDEMKL PSSTESNLSL
FADNILPDYC KQLFKEDFIV NSNGLKSYIF KLRKWRSYFE RLLSKVPKKQ YLEQYSSFLC
EFHHQKFDEI EVPGQYLLHK DNNNSFSCIE RFLPEVELIV GHGVCYRRLS IRSNGGTIHP
FVIQYPSARN SRREERFMQL TRYLNDALAL NCETRRRCLK FYIPAVIPLS SHIRLLEDQP
SSITLQKIYE IYSERNNFSR DDPKELFTNE LSKHMMELNS QISQESTDAE KLANRKQLFS
RRIGMFENIQ KLYSPSTILK DYFSSIFTNY SDLWLFRKNF SYQYACFSFI TYILSINNRI
PAKLVFSRDS GGVWTTEALP SMVSSTPVYH NGEIVPFRFT PNIKEFIGKT CTEGLLGPSI
MAIARALSKP DFDLDMYLGI FIRDDLFWWL AQQTKGVPAD FSMLNKVNSN TDLIMRRVAS
LSQVAYGNLP VNQTAIDYLA QASSSKVLAQ MDVLWAPWL
