TRA2_CAEBR
ID TRA2_CAEBR Reviewed; 1489 AA.
AC Q17307; A8XCP0; Q8ST82; Q8STA3;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 3.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Sex-determining transformer protein 2 {ECO:0000312|WormBase:CBG11194a};
DE Short=Cbr-Tra-2A {ECO:0000312|WormBase:CBG11194a};
DE Short=Cbr-tra-2 {ECO:0000303|PubMed:24098152};
DE Flags: Precursor;
GN Name=tra-2 {ECO:0000312|WormBase:CBG11194a};
GN ORFNames=CBG11194 {ECO:0000312|WormBase:CBG11194a};
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7971272; DOI=10.1093/nar/22.21.4414;
RA Kuwabara P.E., Shah S.;
RT "Cloning by synteny: identifying C. briggsae homologues of C. elegans
RT genes.";
RL Nucleic Acids Res. 22:4414-4418(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND ALTERNATIVE SPLICING.
RC STRAIN=Gujarat;
RX PubMed=8889523; DOI=10.1093/genetics/144.2.597;
RA Kuwabara P.E.;
RT "Interspecies comparison reveals evolution of control regions in the
RT nematode sex-determining gene tra-2.";
RL Genetics 144:597-607(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-137, AND VARIANTS ASN-30; SER-40;
RP GLU-57 AND THR-120.
RC STRAIN=AF16, HK104, HK105, PB800, PB826, and VT847;
RX PubMed=12019226; DOI=10.1093/genetics/161.1.99;
RA Graustein A., Gaspar J.M., Walters J.R., Palopoli M.F.;
RT "Levels of DNA polymorphism vary with mating system in the nematode genus
RT Caenorhabditis.";
RL Genetics 161:99-107(2002).
RN [5]
RP FUNCTION, AND INTERACTION WITH TRA-1.
RX PubMed=11250902; DOI=10.1093/emboj/20.6.1363;
RA Wang S., Kimble J.;
RT "The TRA-1 transcription factor binds TRA-2 to regulate sexual fates in
RT Caenorhabditis elegans.";
RL EMBO J. 20:1363-1372(2001).
RN [6]
RP FUNCTION, AND INTERACTION WITH FEM-3.
RX PubMed=12477393; DOI=10.1016/s0960-9822(02)01333-7;
RA Haag E.S., Wang S., Kimble J.;
RT "Rapid coevolution of the nematode sex-determining genes fem-3 and tra-2.";
RL Curr. Biol. 12:2035-2041(2002).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24098152; DOI=10.1371/journal.pgen.1003850;
RA Guo Y., Chen X., Ellis R.E.;
RT "Evolutionary change within a bipotential switch shaped the sperm/oocyte
RT decision in hermaphroditic nematodes.";
RL PLoS Genet. 9:E1003850-E1003850(2013).
CC -!- FUNCTION: Plays a major role in controlling sexual cell fates
CC (PubMed:8889523, PubMed:11250902, PubMed:24098152). Promotes female
CC development in XX animals where it sequesters one or more of the FEM
CC proteins to the membrane thereby freeing the tra-1 protein (a putative
CC transcription factor) to enter the nucleus and promote female
CC development (By similarity). In XO animals it acts as a receptor for
CC her-1 which prevents it from binding to FEM proteins thereby repressing
CC the activity of tra-1 (By similarity). Negatively regulates male
CC development when bound to fem-3 and is required together with tra-1 for
CC promoting spermatogenesis (PubMed:12477393). Also required for
CC feminizing tra-3 activity (By similarity).
CC {ECO:0000250|UniProtKB:P34709, ECO:0000269|PubMed:11250902,
CC ECO:0000269|PubMed:12477393, ECO:0000269|PubMed:24098152,
CC ECO:0000269|PubMed:8889523}.
CC -!- SUBUNIT: Interacts with tra-1 and fem-3. {ECO:0000269|PubMed:11250902,
CC ECO:0000269|PubMed:12477393}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced.;
CC Name=a;
CC IsoId=Q17307-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: Null hermaphrodite mutants have masculinized germ
CC lines, producing only sperm. Animals make incomplete male tails,
CC producing shorter stubby tails that lack sensory structures called
CC rays. {ECO:0000269|PubMed:24098152}.
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DR EMBL; U59879; AAB49333.1; -; mRNA.
DR EMBL; HE601002; CAP30389.1; -; Genomic_DNA.
DR EMBL; AF491464; AAM09704.1; -; Genomic_DNA.
DR EMBL; AF491465; AAM09705.1; -; Genomic_DNA.
DR EMBL; AF491466; AAM09706.1; -; Genomic_DNA.
DR EMBL; AF491467; AAM09707.1; -; Genomic_DNA.
DR EMBL; AF491468; AAM09708.1; -; Genomic_DNA.
DR EMBL; AF491469; AAM09709.1; -; Genomic_DNA.
DR PIR; S72250; S72250.
DR AlphaFoldDB; Q17307; -.
DR IntAct; Q17307; 1.
DR STRING; 6238.CBG11194; -.
DR PRIDE; Q17307; -.
DR WormBase; CBG11194a; CBP38209; WBGene00032357; Cbr-tra-2.
DR eggNOG; ENOG502R9RT; Eukaryota.
DR HOGENOM; CLU_005001_0_0_1; -.
DR InParanoid; Q17307; -.
DR OMA; SIWTIER; -.
DR OrthoDB; 1388750at2759; -.
DR Proteomes; UP000008549; Chromosome II.
DR GO; GO:0016021; C:integral component of membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0018992; P:germ-line sex determination; IMP:UniProtKB.
DR GO; GO:0040021; P:hermaphrodite germ-line sex determination; IEA:InterPro.
DR GO; GO:0042001; P:hermaphrodite somatic sex determination; IEA:InterPro.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR032848; Ce-Tra-2.
DR PANTHER; PTHR39365; PTHR39365; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Differentiation; Membrane;
KW Receptor; Reference proteome; Sexual differentiation; Signal;
KW Spermatogenesis; Transmembrane; Transmembrane helix.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..1489
FT /note="Sex-determining transformer protein 2"
FT /id="PRO_0000022573"
FT TRANSMEM 454..474
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 489..509
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 513..533
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 600..620
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 622..642
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 749..769
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 931..951
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 958..978
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 986..1006
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1041..1061
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1066..1086
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1138..1288
FT /note="Interaction with fem-3"
FT /evidence="ECO:0000250"
FT REGION 1143..1176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1233..1393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1402..1423
FT /note="MX regulatory domain; required for tra-1 binding"
FT REGION 1412..1489
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1275..1302
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1423..1438
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1439..1453
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1459..1483
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 30
FT /note="T -> N (in strain: HK104, HK105, PB800 and PB826)"
FT /evidence="ECO:0000269|PubMed:12019226"
FT VARIANT 40
FT /note="I -> S (in strain: HK104, HK105, PB800 and PB826)"
FT /evidence="ECO:0000269|PubMed:12019226"
FT VARIANT 57
FT /note="Q -> E (in strain: HK104, HK105, PB800 and PB826)"
FT /evidence="ECO:0000269|PubMed:12019226"
FT VARIANT 120
FT /note="A -> T (in strain: HK104, HK105, PB800 and PB826)"
FT /evidence="ECO:0000269|PubMed:12019226"
FT CONFLICT 55
FT /note="T -> M (in Ref. 1; AAB49333)"
FT /evidence="ECO:0000305"
FT CONFLICT 825..830
FT /note="GRRVLI -> WKKSFDRYERRIYQ (in Ref. 1; AAB49333)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1489 AA; 171114 MW; 979CABEAADCE5949 CRC64;
MKLAFNKLLV ASVVFTVLSF GLLLASLFTT TATTPSEWTI LLPEFRFPVN KKQTTEQFLV
EKIVHEHEEG EDVRSALYLT HHGYFMNAIA NMKVTYRQKS YTVNDVCFKP HSAIFENVPA
PENIDKLPAY FQRLLLEMQR LSPCLIVTPL NCFYDSYHIH SEISNWNAST DYLNRRLRNS
YLEAIEEKDS RPYVKSTYGP ELIKEWARHM FAIPSKPLSN FSKSDLYSRV KTWLSSIAAR
KKICADPMRS CDETLDAENY FNVCTVMQQI NDYDERRKQR LKFQLEYGDE EFTTRLDCVE
DREKFIEWMQ ERNLRDMLKL FASSVEIPDH KEIPNQVCDG IYHDLDTSSG LELFRGARSF
SNNTSAYDTI NVELGFMTPE NLLTTMRHSD FVNGFESIWT IERARELLNE FRLALKVEVT
KFSESRSSRR VKVTTRIVNQ IEEEGSDEEM EYHMIYFILG ACALMVALFA AFAFSEAFLT
SLSMFLLRGF ITGLLFIFLC KSGGLILIDS NFLCYITMHL AFNLVMTARV TFICYRIGGC
VQSEKDFVKS NFSSLGSVPV DSLKEDSCKR HVQYVLAKYT KFQVAQDAYS EEPFEKLPKY
WFLIAIVLVP VIGVYWFFID SDVQKICIVL LPAFLIAAFE EMRVKNQLLR ERRIKKAIQR
LQKEENTRIM SRGEIDNLLS GNAELSGEKS HYESKQGVLH HGSAGGLFEL SRSTYDVSLI
MAYPNQMIRN LRLCALGAYF RLFKMKYCAV VVSSVAALLI LLSIGLLFIP VQRSSVPKEL
QQDELSIDFA IPNVSSSSWE SINEYLEEFN SEIDSITNLQ TITNGRRVLI NSTSKINNFL
KWVDDEPISW YLTAPLTRPY RKTHLPNPFR FQFRYGFDSI QKSTIIDVVE RIDTLLTKYT
ETLSFPKAIG FLYEHYHQKA VVWNSFAYHE IFAAAVLAGF FSIIVVFFSI GPVVLPTLAF
AFFVVGNRLE IAAIVSLFSL EYHQCYTNVA VFVGFLAAWT PFCDLARFRG RLLYKDQTRR
TPELATQRRI RVPHVAAVDT VQIFAIFLTA TILLIVITAI IPQFRAFFIP TVILLITLLL
AVFNSLAVSL AAYQMFEHEV RHCYHDQLQS LTTTGKVCDM TRKKLLPREE DLSIPMEEFS
IRPTENTKHY APRPIDNSDP PEQAADEEVV NQDPSMEAAR RQYVEFTHRT TGMPIELINQ
FVDNFPVFNV PANFLPNYFA LGGAPLDANN GVLLRQPGIA PPPRPNREED EEERFGLGGG
EDDDSYPSSG DDIGDPAKEQ QEVTDDVATR YKEEEVRKKV QPAVPNYDDP NVPGPSNPVP
RQVEQVSREA PEDSPNREPR ILVYQRPPRL HEIPQISHGR NPLHDPPSME EYVQKYDDPN
QPPSRRADQY PPSFTPAMVG YCEDVYWKYN ERNLPDNVPM PPRPRDWDQR RLVELPPPED
FDEVPPPGRS AIPIPPGAIR LRERRREQHL REQEARRNRP ESPDDTPGL
