TRA4_CAEEL
ID TRA4_CAEEL Reviewed; 543 AA.
AC Q20709;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=Zinc finger protein tra-4 {ECO:0000305};
DE AltName: Full=PLZF-like protein tra-4 {ECO:0000303|PubMed:17011494};
GN Name=tra-4 {ECO:0000312|WormBase:F53B3.1};
GN ORFNames=F53B3.1 {ECO:0000312|WormBase:F53B3.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, INTERACTION WITH HDA-1 AND NASP-1, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND MUTAGENESIS OF 381-TYR--GLU-543 AND HIS-493.
RX PubMed=17011494; DOI=10.1016/j.devcel.2006.07.015;
RA Grote P., Conradt B.;
RT "The PLZF-like protein TRA-4 cooperates with the Gli-like transcription
RT factor TRA-1 to promote female development in C. elegans.";
RL Dev. Cell 11:561-573(2006).
CC -!- FUNCTION: Probable transcription factor (PubMed:17011494). Promotes
CC normal hermaphrodite (XX) development, in concert with histone
CC deacetylase hda-1 and nasp-1, perhaps as components of a complex
CC (PubMed:17011494). May cooperate with transcription factor tra-1 to
CC repress male-specific genes in hermaphrodites (PubMed:17011494).
CC Synthetic multivulva (synMuv) class B protein, required to repress the
CC induction of vulval development by let-60 Ras signaling
CC (PubMed:17011494). {ECO:0000269|PubMed:17011494}.
CC -!- SUBUNIT: Interacts with histone deacetylase hda-1 (PubMed:17011494).
CC May interact with nasp-1 (PubMed:17011494).
CC {ECO:0000269|PubMed:17011494}.
CC -!- INTERACTION:
CC Q20709; Q22174: CELE_T04H1.2; NbExp=3; IntAct=EBI-315012, EBI-311938;
CC Q20709; Q9NAN2: par-6; NbExp=5; IntAct=EBI-315012, EBI-318782;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17011494}.
CC -!- DEVELOPMENTAL STAGE: Expressed widely from the 28-cell stage of
CC embryogenesis and throughout development and adult life.
CC {ECO:0000269|PubMed:17011494}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; BX284606; CCD61859.1; -; Genomic_DNA.
DR PIR; T16449; T16449.
DR RefSeq; NP_508494.1; NM_076093.3.
DR AlphaFoldDB; Q20709; -.
DR DIP; DIP-25182N; -.
DR IntAct; Q20709; 21.
DR STRING; 6239.F53B3.1; -.
DR EPD; Q20709; -.
DR PaxDb; Q20709; -.
DR PeptideAtlas; Q20709; -.
DR EnsemblMetazoa; F53B3.1.1; F53B3.1.1; WBGene00018740.
DR EnsemblMetazoa; F53B3.1.2; F53B3.1.2; WBGene00018740.
DR UCSC; F53B3.1; c. elegans.
DR WormBase; F53B3.1; CE28444; WBGene00018740; tra-4.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00970000196688; -.
DR HOGENOM; CLU_030063_0_0_1; -.
DR InParanoid; Q20709; -.
DR OMA; EMVEFKQ; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q20709; -.
DR Reactome; R-CEL-111367; SLBP independent Processing of Histone Pre-mRNAs.
DR Reactome; R-CEL-73856; RNA Polymerase II Transcription Termination.
DR Reactome; R-CEL-77588; SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs.
DR SignaLink; Q20709; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00018740; Expressed in embryo and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0042826; F:histone deacetylase binding; IPI:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0042001; P:hermaphrodite somatic sex determination; IMP:UniProtKB.
DR GO; GO:0040027; P:negative regulation of vulval development; IGI:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR SMART; SM00355; ZnF_C2H2; 7.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Metal-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..543
FT /note="Zinc finger protein tra-4"
FT /id="PRO_0000452695"
FT ZN_FING 218..241
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 327..350
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 381..406
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255"
FT ZN_FING 413..436
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 442..464
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 470..493
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 495..518
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 381..543
FT /note="Missing: In bc250; masculinization of (XX)
FT hermaphrodites. Male-specific cephalic companion neurons
FT (CEMs) found in 97% hermaphrodites, and hermaphrodite-
FT specific neurons (HSNs) found in only 5% of hermaphrodites.
FT On a lin-8 (SynMuv Class A gene) mutant background, induces
FT multiple vulval invaginations."
FT /evidence="ECO:0000269|PubMed:17011494"
FT MUTAGEN 493
FT /note="H->Y: In bc45; weak masculinization of
FT hermaphrodites. Male-specific cephalic companion neurons
FT (CEMs) found in 67% hermaphrodites, and hermaphrodite-
FT specific neurons (HSNs) found in only 24% of
FT hermaphrodites."
FT /evidence="ECO:0000269|PubMed:17011494"
SQ SEQUENCE 543 AA; 63784 MW; 23A5307414BB61C1 CRC64;
MDDPNQCTIK QEDSITRPRP TEAPTIQNLK QEPAIEEGSS STMDNMLMLT IDTNQANWQG
TYDDDEMDLN NKTDLIPLLE TEKTTINEDE LYDDEEDDDD DEEIKKGIGF ELLAQALGMS
AKVPVEKEEP EKERAKLSGV GEFMKQIRGE IKPIQKERIV LDELGFRVRD PSKFPPCRIA
EVQQTLTLAD HQDGIDLPPP NAPTDVRIVR KLIRQKMVRC KKCKNRFIEK NIYERHLRDK
HPDLYEEYIR EQEEEVELQR LEEIEANRIE ELQTGGFIPP ENEISQPSED PNYIPLPGEN
NGGLVPRFDY YGRIKQLKRP YKKKVSPQCP FCDKRFRNEF SLKKHFAKKH EEMVEFQQCL
KCFKCVENDA EMANHDCELT YVCFECTPIR NLCTDNRLLN HRKKFHRGAN SGFRCSFCNM
KFLTPRKLRK HKKMSHVFTK TFQCHFCEEI FISEVAVMTH ERMHTGIIKF ECKVCDFRAN
RYTAMEEHKR DEHGYVCAIC HERHAEYPEM KHHVYEEHGG YLAADEPTAY VETPRMWILY
KGE
