ID   TRA6_GEOSE              Reviewed;         400 AA.
AC   Q45618;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Putative transposase for insertion sequence element IS5376;
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CU21;
RX   PubMed=8382825; DOI=10.1006/plas.1993.1001;
RA   Xu K., He Z.-Q., Mao Y.-M., Shen R.-Q., Sheng Z.-J.;
RT   "On two transposable elements from Bacillus stearothermophilus.";
RL   Plasmid 29:1-9(1993).
CC   -!- FUNCTION: Involved in the transposition of the insertion sequence.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the transposase IS21/IS408/IS1162 family.
CC       {ECO:0000305}.
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DR   EMBL; X67861; CAA48045.1; -; Genomic_DNA.
DR   PIR; S23888; S23888.
DR   AlphaFoldDB; Q45618; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000150; F:DNA strand exchange activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR017894; HTH_IS21_transposase_type.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR006120; Resolvase_HTH_dom.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF02796; HTH_7; 1.
DR   Pfam; PF00665; rve; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50531; HTH_IS21; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Transposable element; Transposition.
FT   CHAIN           1..400
FT                   /note="Putative transposase for insertion sequence element
FT                   IS5376"
FT                   /id="PRO_0000075464"
FT   DOMAIN          5..67
FT                   /note="HTH IS21-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00615"
FT   DOMAIN          113..287
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT   DNA_BIND        20..39
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00615"
FT   REGION          35..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   400 AA;  46818 MW;  E1EB797F0D402EA2 CRC64;
     MITRGEFFMI KEMYERGMSI SDIARELGID RKTVRKYIHS PNPPSKSKRK QRKSKLDPFK
     PYLQKRMLED GVFNSEKLFF EIRQQGYTGG KTILKDYMKP FRETAKKKYT VRYETLPGEQ
     MQVDWKEVGE VVIEGKKVKL SLFVATLGYS RMKYAVFTTS QDQEHLMECL IQSFKYFGGV
     PKKVLFDNMK TVTDGREQGV VKWNQRFSEF ASYYGFIPKV CRPYRAQTKG KVERAIQYIM
     DHFYVGTAFE SIEELNFLLH RWLDQVANRK PNATTGISPQ ERWAEESLKP LPLKDYDTSY
     LSYRKVHWDG SFSYKGEQWL LSAEYAGKEI LVKERLNGDI RLYFRGEEIS HVDQQKKVIS
     FAEKIKKKQT EMAATISPVS VEVDTRPLSV YDAFLRGESS