ID   TRA6_SHIFL              Reviewed;         507 AA.
AC   P0A135; Q57541;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Transposase for insertion sequences IS1326/IS1353;
GN   Name=istA;
OS   Shigella flexneri.
OG   Plasmid IncFII R100 (NR1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TRANSPOSON=In2;
RX   PubMed=8755869; DOI=10.1128/jb.178.15.4429-4437.1996;
RA   Brown H.J., Stokes H.W., Hall R.M.;
RT   "The integrons In0, In2, and In5 are defective transposon derivatives.";
RL   J. Bacteriol. 178:4429-4437(1996).
CC   -!- FUNCTION: Required for the transposition of the insertion element.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the transposase IS21/IS408/IS1162 family.
CC       {ECO:0000305}.
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DR   EMBL; U42226; AAC53729.1; -; Genomic_DNA.
DR   EMBL; U38187; AAA79725.1; -; Genomic_DNA.
DR   RefSeq; WP_001324342.1; NZ_UDTD01000066.1.
DR   AlphaFoldDB; P0A135; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR017894; HTH_IS21_transposase_type.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PROSITE; PS50531; HTH_IS21; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Plasmid; Transposable element;
KW   Transposition.
FT   CHAIN           1..507
FT                   /note="Transposase for insertion sequences IS1326/IS1353"
FT                   /id="PRO_0000075469"
FT   DOMAIN          6..68
FT                   /note="HTH IS21-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00615"
FT   DOMAIN          122..302
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
SQ   SEQUENCE   507 AA;  57965 MW;  C90AA7512A5CCA1F CRC64;
     MINVAILSAI RRWHFRDGAS IREIARRSGL SRNTVRKYLQ SKVVEPQYPA RDSVGKLSPF
     EPKLRQWLST EHKKTKKLRR NLRSMYRDLV ALGFTGSYDR VCAFARQWKD SEQFKAQTSG
     KGCFIPLRFA CGEAFQFDWS EDFARIAGKQ VKLQIAQFKL AHSRAFVLRA YYQQKHEMLF
     DAHWHAFQIF GGIPKRGIYD NMKTAVDSVG RGKERRVNQR FTAMVSHYLF DAQFCNPASG
     WEKGQIEKNV QDSRQRLWQG APDFQSLADL NVWLEHRCKA LWSELRHPEL DQTVQEAFAD
     EQGELMALPN AFDAFVEQTK RVTSTCLVHH EGNRYSVPAS YANRAISLRI YADKLVMAAE
     GQHIAEHPRL FGSGHARRGH TQYDWHHYLS VLQKKPGALR NGAPFAELPP AFKKLQSILL
     QRPGGDRDMV EILALVLHHD EGAVLSAVEL ALECGKPSKE HVLNLLGRLT EEPPPKPIPI
     PKGLRLTLEP QANVNRYDSL RRAHDAA