ID   TRA8_CUPMC              Reviewed;         339 AA.
AC   P37248; Q5NV33;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 2.
DT   25-MAY-2022, entry version 120.
DE   RecName: Full=Transposase for insertion sequence element IS1086;
GN   Name=IS1086; OrderedLocusNames=Rmet_2842, Rmet_4004, Rmet_6282;
GN   ORFNames=RMe0033;
OS   Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS   CH34) (Ralstonia metallidurans).
OG   Plasmid megaplasmid CH34, and Plasmid pMOL28.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=266264;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1334071; DOI=10.1128/jb.174.24.8133-8138.1992;
RA   Dong Q., Sadouk A., van der Lelie D., Taghavi S., Ferhat A., Nuyten J.M.,
RA   Borremans B., Mergeay M., Toussaint A.;
RT   "Cloning and sequencing of IS1086, an Alcaligenes eutrophus insertion
RT   element related to IS30 and IS4351.";
RL   J. Bacteriol. 174:8133-8138(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RC   PLASMID=megaplasmid CH34, and pMOL28;
RX   PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA   Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA   Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA   Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT   "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT   master survivalist in harsh and anthropogenic environments.";
RL   PLoS ONE 5:E10433-E10433(2010).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34; PLASMID=pMOL28;
RA   van der Lelie D., Monchy S., Taghavi S., McCorkle S., Dunn J.,
RA   Benotmane M., Vallaeys T., Lapidus A., Mergeay M.;
RT   "Sequence and features of the Ralstonia metallidurans CH34 heavy metal
RT   plasmids pMOL28 and pMOL30.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for the transposition of the insertion element.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the transposase IS30 family. {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-15 is the initiator.
CC       {ECO:0000305}.
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DR   EMBL; X58441; CAA41347.1; -; Genomic_DNA.
DR   EMBL; CP000352; ABF09715.1; -; Genomic_DNA.
DR   EMBL; CP000353; ABF10872.1; -; Genomic_DNA.
DR   EMBL; X90708; CAI30179.1; -; Genomic_DNA.
DR   EMBL; CP000355; ABF13141.1; -; Genomic_DNA.
DR   RefSeq; WP_011239917.1; NC_007974.2.
DR   RefSeq; YP_161657.1; NC_006525.1.
DR   AlphaFoldDB; P37248; -.
DR   EnsemblBacteria; ABF09715; ABF09715; Rmet_2842.
DR   EnsemblBacteria; ABF10872; ABF10872; Rmet_4004.
DR   EnsemblBacteria; ABF13141; ABF13141; Rmet_6282.
DR   KEGG; rme:Rmet_2842; -.
DR   KEGG; rme:Rmet_4004; -.
DR   KEGG; rme:Rmet_6282; -.
DR   eggNOG; COG2826; Bacteria.
DR   HOGENOM; CLU_035706_0_0_4; -.
DR   OMA; RSVIWRE; -.
DR   OrthoDB; 1780129at2; -.
DR   Proteomes; UP000002429; Chromosome.
DR   Proteomes; UP000002429; Plasmid megaplasmid CH34.
DR   Proteomes; UP000002429; Plasmid pMOL28.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004803; F:transposase activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IEA:InterPro.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR025246; IS30-like_HTH.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR001598; Transposase_IS30_CS.
DR   Pfam; PF13936; HTH_38; 1.
DR   Pfam; PF00665; rve; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
DR   PROSITE; PS01043; TRANSPOSASE_IS30; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Plasmid; Reference proteome;
KW   Transposable element; Transposition.
FT   CHAIN           1..339
FT                   /note="Transposase for insertion sequence element IS1086"
FT                   /id="PRO_0000211894"
FT   DOMAIN          176..329
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT   CONFLICT        102
FT                   /note="A -> G (in Ref. 1; CAA41347)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   339 AA;  38101 MW;  0CC50CC79AF79DE9 CRC64;
     MTRTKYQQLQ PEERMRIEIW KAEDVSLRAM ARRLGRAPST LMRELRRNAT ARGGYGAMSA
     QACRTQRLKA SRPVAKLAPD GVLWGVVRHF LDQKWSPQEI SATLKRAFPD QPDLNVSHET
     IYNAIYAYPR GELRRQLIAC LRQARTKRLP RSRGTDRRGQ IPDMVSIHVR PPEVNDRLMP
     GHWEGDLIKG AGNQSAVGVL VERMSRAVLL VKMPDATAAS ALAGFTGKLQ SLVAPLRQTL
     TYDQGREMAR HAELSAATGV RVYFCDPHSP WQRGTCENTN GLLRQYLPKG TDLSVYSQEE
     LDAIADSLNG RPRKTLNWHS PLQVLAQVLA NPTDRLPVQ