ID   TRAB_AGRFC              Reviewed;         421 AA.
AC   Q44351;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Conjugal transfer protein TraB;
GN   Name=traB; OrderedLocusNames=Atu6129; ORFNames=AGR_pTi_241;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OG   Plasmid pTiC58.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8763953; DOI=10.1128/jb.178.14.4233-4247.1996;
RA   Farrand S.K., Hwang I., Cook D.M.;
RT   "The tra region of the nopaline-type Ti plasmid is a chimera with elements
RT   related to the transfer systems of RSF1010, RP4, and F.";
RL   J. Bacteriol. 178:4233-4247(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: Enhances conjugal transfer of the Ti plasmid.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK91093.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF010180; AAC17208.1; -; Genomic_DNA.
DR   EMBL; AE007871; AAK91093.2; ALT_INIT; Genomic_DNA.
DR   PIR; AG3243; AG3243.
DR   PIR; T03421; T03421.
DR   RefSeq; NP_396652.4; NC_003065.3.
DR   AlphaFoldDB; Q44351; -.
DR   EnsemblBacteria; AAK91093; AAK91093; Atu6129.
DR   KEGG; atu:Atu6129; -.
DR   PATRIC; fig|176299.10.peg.5336; -.
DR   HOGENOM; CLU_042375_0_0_5; -.
DR   OMA; GITGWAH; -.
DR   Proteomes; UP000000813; Plasmid Ti.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 3.60.110.10; -; 1.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR036526; C-N_Hydrolase_sf.
DR   InterPro; IPR016707; Conjugal_tfr_TraB_rhizob.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   PIRSF; PIRSF017932; Conjugal_transfer_TraB_rhizob; 1.
DR   SUPFAM; SSF56317; SSF56317; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
PE   4: Predicted;
KW   Cell membrane; Conjugation; Membrane; Plasmid; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..421
FT                   /note="Conjugal transfer protein TraB"
FT                   /id="PRO_0000065596"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        103..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        144..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        169..189
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        193..213
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        287..307
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        366..386
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        388..408
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          222..421
FT                   /note="CN hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
SQ   SEQUENCE   421 AA;  45195 MW;  3518583863B0AFCD CRC64;
     MIHDTSARSR TAVFSAWRGR CSRLTRDRWR VALLILLSIA VGAVGWSGEA LLLPTAMLFP
     LLWAQSPSRL VAGAVSAGYF LTASRGLPQG VANFYAADFW HGLLLWLAAS AGFVAVHAAF
     WPARLQKRLP GRGALGWGKP VRYLAAAVLM GLPPFGITGW AHPLTAAGIL FPGFGWWGLG
     ATTAGLAMMT SRYWPAAAIA LGGFWFWSAA TWTQPVLPDG WKGVDLEQGQ TLGRDGSLDH
     HRDLIATVRA AAGAETRVIV LPESALGLWT PTVARLWQAG LRGADVTVIA GAAVIDPGGY
     DNVMVTVSEG ETRILYRERM PIPVSMWQPW LQWTGQGGGA QAHFFANPAV DLAGTRIAPL
     ICYEQLIVWP ILHSMLFSPA AIVATGNGWW TEGTSIVAIQ QAGVIAWAKL FGRPVVTAFN
     T