ID   TRAC4_ECOLX             Reviewed;        1061 AA.
AC   P27189; P27184;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=DNA primase TraC;
DE            EC=2.7.7.-;
DE   AltName: Full=Replication primase;
GN   Name=traC;
OS   Escherichia coli.
OG   Plasmid IncP-alpha RP4.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-14 AND
RP   316-333.
RC   STRAIN=ATCC 33694 / HB101;
RX   PubMed=1818755; DOI=10.3109/10425179109039685;
RA   Miele L., Strack B., Kruft V., Lanka E.;
RT   "Gene organization and nucleotide sequence of the primase region of IncP
RT   plasmids RP4 and R751.";
RL   DNA Seq. 2:145-162(1991).
CC   -!- FUNCTION: Required for autonomous replication in E.coli. Transferred
CC       into the recipient cell during bacterial conjugation. Catalyzes the
CC       synthesis of short oligoribonucleotide primers with CpA or pCpA at
CC       their 5'-termini on a single-stranded template DNA.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=TraC-1;
CC         IsoId=P27189-1; Sequence=Displayed;
CC       Name=TraC-2;
CC         IsoId=P27189-2; Sequence=VSP_018716;
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DR   EMBL; X59793; CAA42455.1; -; Genomic_DNA.
DR   EMBL; X59793; CAA42456.1; -; Genomic_DNA.
DR   PIR; S37667; S37667.
DR   RefSeq; WP_011205813.1; NZ_NJTR01000055.1.
DR   AlphaFoldDB; P27189; -.
DR   SMR; P27189; -.
DR   BindingDB; P27189; -.
DR   ChEMBL; CHEMBL1075082; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   CDD; cd01029; TOPRIM_primases; 1.
DR   InterPro; IPR013610; ArdC_N.
DR   InterPro; IPR043764; DUF5710.
DR   InterPro; IPR041459; MPTase-PolyVal.
DR   InterPro; IPR034154; TOPRIM_DnaG/twinkle.
DR   InterPro; IPR006171; TOPRIM_domain.
DR   Pfam; PF08401; ArdcN; 1.
DR   Pfam; PF18974; DUF5710; 2.
DR   Pfam; PF18818; MPTase-PolyVal; 1.
DR   Pfam; PF13362; Toprim_3; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   PROSITE; PS50880; TOPRIM; 1.
PE   1: Evidence at protein level;
KW   Alternative initiation; Direct protein sequencing; DNA replication;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Plasmid;
KW   Transcription; Transferase.
FT   CHAIN           1..1061
FT                   /note="DNA primase TraC"
FT                   /id="PRO_0000007251"
FT   DOMAIN          850..938
FT                   /note="Toprim"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00995"
FT   REGION          1034..1061
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..315
FT                   /note="Missing (in isoform TraC-2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018716"
SQ   SEQUENCE   1061 AA;  116722 MW;  ABC344D2811B9B31 CRC64;
     MAEVKKPFHE QVAERLIEQL KAGTAPWQKP WEPGMPGSFI PLNPTTGKRY KGINAIQLMA
     QGHADPRWMT YKQAAAAGAQ VRRGEKGTPI QYWKFSEEQT KTDEQTGKPV LDANGDPVKV
     TVQLERPRVF FATVFNAEQI DGLPPLERKE QTWSAVERAE HILAASGATI RHGEHDRAFY
     RPSTDSIHLP DKGQFPSADN YYATALHELG HWTGHPSRLD RDLAHPFGSE GYAKEELRAE
     IASMILGDEL GIGHDPGQHA AYVGSWIKAL QEDPLEIFRA AADAEKIQDF VLAFEQKQIQ
     EQTTQQAIEP AQGATMEQQQ DQVARPAIAP ADELIAQTLR MYRAGAEPAE GNQSLAALTE
     TTLGFELPAD WTGRVQVQAN VEVEHDGERS VVPAGDREPE FWGVYANHAW GGHQWLADFA
     GPDAQTNAEA LADRLAVIDA YATANEYEQA AKFARIHEER VRRDPNSTDE DRVAAKEARK
     AAEGTAMLHD EDLQRRIADY EREQQEMAQA MNAAEQPAAA QAPAKPERAY LNVPFKEKDE
     VKALGARWDR QERAWYVPAG VDPAPFAKWA REGATAAVEA RAEAQPTQPT AERPNAAQER
     VYLAVPYGER QVAKAAGAQW DKVAKSWYAG PNADMGKLQR WLPDNVPTQQ SPAVTPEDEF
     AEALKSMGCV VTPGGEHPIM DGKKHRIETE GDKKGEKSGF YVGHLDGHPA GYIKNNRTGV
     EMKWKAKGYA LDPAEKAKMQ AEAAAKLAAR AEEQERQHEA TAQRIGRQAQ SLVPITEPTP
     YLRDKGLQVH AGVLTDQEGQ KTYIPAYDAD GKQWTMQYIQ EDGTKRFAKD SRKEGCFHVV
     GGMDALAAAP ALVIGEGYAT AATVAEALGH ATVAAFDSGN LQAVAEALHA KFPDKPVVIA
     GDDDRQVQIT QGVNPGRTKA QEAAKAVGGK AIFPIFAPGE NAYPKELPPI TPENYRNHLH
     AEKRLADAAA GKVQLSEADT AKLKESLLND GQLAALSNMK KHTDFNDLSE RSSLGKDGVE
     RQVRSAVGKV LLDEGQRQKV QQLKQQDIEQ QEQRQRRART Y