TRAC5_ECOLX
ID TRAC5_ECOLX Reviewed; 1448 AA.
AC P27190; P27185; P27191;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=DNA primase TraC;
DE EC=2.7.7.-;
DE AltName: Full=Replication primase;
GN Name=traC;
OS Escherichia coli.
OG Plasmid IncP-beta R751.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-11; 219-234
RP AND 702-714.
RC STRAIN=ATCC 33694 / HB101;
RX PubMed=1818755; DOI=10.3109/10425179109039685;
RA Miele L., Strack B., Kruft V., Lanka E.;
RT "Gene organization and nucleotide sequence of the primase region of IncP
RT plasmids RP4 and R751.";
RL DNA Seq. 2:145-162(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Thomas C.M.;
RL Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for autonomous replication in E.coli. Transferred
CC into the recipient cell during bacterial conjugation. Catalyzes the
CC synthesis of short oligoribonucleotide primers with CpA or pCpA at
CC their 5'-termini on a single-stranded template DNA.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=3;
CC Name=TraC-2;
CC IsoId=P27190-1; Sequence=Displayed;
CC Name=TraC-3;
CC IsoId=P27190-2; Sequence=VSP_018717;
CC Name=TraC-4;
CC IsoId=P27190-3; Sequence=VSP_018718;
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DR EMBL; X59794; CAA42460.1; -; Genomic_DNA.
DR EMBL; U67194; AAC64468.1; -; Genomic_DNA.
DR PIR; S37669; S37669.
DR RefSeq; WP_006122509.1; NZ_MW574946.1.
DR AlphaFoldDB; P27190; -.
DR SMR; P27190; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd01029; TOPRIM_primases; 1.
DR InterPro; IPR013610; ArdC_N.
DR InterPro; IPR043764; DUF5710.
DR InterPro; IPR041459; MPTase-PolyVal.
DR InterPro; IPR034154; TOPRIM_DnaG/twinkle.
DR InterPro; IPR006171; TOPRIM_domain.
DR Pfam; PF08401; ArdcN; 1.
DR Pfam; PF18974; DUF5710; 2.
DR Pfam; PF18818; MPTase-PolyVal; 1.
DR Pfam; PF13362; Toprim_3; 1.
DR SMART; SM00493; TOPRIM; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 1: Evidence at protein level;
KW Alternative initiation; Direct protein sequencing; DNA replication;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Plasmid;
KW Transcription; Transferase.
FT CHAIN 1..1448
FT /note="DNA primase TraC"
FT /id="PRO_0000007253"
FT DOMAIN 1237..1325
FT /note="Toprim"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00995"
FT REGION 844..915
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 952..982
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1414..1448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 844..868
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 884..903
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1421..1448
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..701
FT /note="Missing (in isoform TraC-4)"
FT /evidence="ECO:0000305"
FT /id="VSP_018718"
FT VAR_SEQ 1..218
FT /note="Missing (in isoform TraC-3)"
FT /evidence="ECO:0000305"
FT /id="VSP_018717"
SQ SEQUENCE 1448 AA; 158951 MW; 1C0EAE67CD0D4ED4 CRC64;
MPITKAEAQG VTRAFVRDYP GALELAYKFR EDAAELYGPR AAEVPADMKG GYVPKETLHA
GRAYRGRVDV PLQNVESASD LLMTLRHEVL GHYGANTFAP GEKRALLDGL AAARNEPTLK
PLWDDVNRRY AGQSLDVRAE EVFALHCEGI EPSQHQVADQ VQQRGQQSFT ETCIARVRPM
QADDLHNIVC MVAQGLRDRS RTQQNFPQFN ELFRRDENME PKKPFHEVVA EKLIEQLKAG
TAPWQKPWEP GEPNAYLPMN PTTGKRYKGI NAIHLMAQGR SDARWMTYKQ AAAVGAQVRK
GEKGTPVQYW KFSEEQDKLD DSGRPVLDAK GQPVKETVML ERPRVFFATV FNGEQIDGLP
PLQPKKEQTW NAVERAEHIL KASGATITHA AGDRAFYRPS TDSITLPERG QFPSSDRYYA
TALHELGHWT GHASRLDRDL AHPFGSEGYA KEELRAEIAS MIVGDELGIG HDPGQHAAYV
GSWIKALQDE PLEVFRAAAD AEKIHDYVLA FEQKQVQEQD QQQSQAQDEA VAQALAVDIA
EVLDNPDVSF SHYQAFQGDT LEDALRSRGL ETVGSITGTD PEQFYAVAHD RLSPVFGIDP
SHTDTDNAYL ERKGLAQEFA NMAEQLHLAQ QLQQHGEQIV SSIDAEARWS DGQRIFAFHD
QDGEPHQVRS LDELNNYAPD QLMALPALTQ QQAAVADQEA NMTPPTIDQA AALLAAHPAD
AVQGIEQAAA ARRQLAAGEI DGQAFADATR QHLGVELPPD WSGELRIVGV AEQDGQTVDA
AQAGIEPQAF QVYARKADAQ FGEDAFAFVA GTRTEGQAEA LAERLHLVDA LGTDNQHERA
AKLARVQEER VRRDPNATEE DISAAKEARK TAEASAMLND SDAQRRAAEL ERQERDRQQA
QPQAEKPERQ YINVPYKEKD EAKSLGARWD RQQQSWYVPP GTDAAPFAKW AQGAATAAVE
PRSAQPAPEA QGEAQKPAQQ AQQARQYLAV PYEQRNAAKA AGALWDKAAK SWYVGPRADA
AKLERWKPEN VQAQQGPAMT PREEFAEAMR SAGLFTGSNA QGDHPIMDGK RHRVPVEGGK
KGALDGFYVG HLDGHPAGRI INNKTGTDIT WKSKGYALSD QEKAKLQAEA AEKLAQRAVE
QDKAQEATAQ RVGRQMADLV PIEQPTPYLQ AKGIQAQAGV MTDREGQKTY IPAFDAEGKQ
WTMQYIQEDG TKRFAKDSKK EGCFHPVGGM DALAAAPALV ISEGYATAAQ VAEAVGHATV
AAFDSGNLEA VAKALHAKFP DKPVIIAGDD DRHLVMTHGN NPGREKAEAA AQAVGGKAIF
PIFAPAENTY PRDLPAITPD SFKTHLRAEQ RLADAAAGKV ELAGDEAAKL KASMLSGAQI
AALSTMKQHT DFNDLAHKSE LGIEGVKRQI GAAISQVQRD EQQHQEQKHV EKKQQQIEQR
PRRAARIG
