ID   TRAD2_ECOLX             Reviewed;         738 AA.
AC   P22708;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Coupling protein TraD;
DE   AltName: Full=DNA transport protein TraD;
GN   Name=traD;
OS   Escherichia coli.
OG   Plasmid IncFII R100 (NR1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2164585; DOI=10.1016/0022-2836(90)90145-c;
RA   Yoshioka Y., Fujita Y., Ohtsubo E.;
RT   "Nucleotide sequence of the promoter-distal region of the tra operon of
RT   plasmid R100, including traI (DNA helicase I) and traD genes.";
RL   J. Mol. Biol. 214:39-53(1990).
CC   -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC       of ssDNA plasmid from a donor to a recipient cell. It is the central
CC       mechanism by which antibiotic resistance and virulence factors are
CC       propagated in bacterial populations. Couples the transferosome to a
CC       type IV secretion system. Probably forms a pore through which single-
CC       stranded plasmid DNA is transferred to the secretion system. Plasmid
CC       specificity is conferred by the TraD-TraM pair (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with relaxosome component TraM. May form a hexamer
CC       in the membrane (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TrwB coupling protein family. {ECO:0000305}.
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DR   EMBL; X55815; CAA39336.1; -; Genomic_DNA.
DR   PIR; S10659; S10659.
DR   RefSeq; WP_000009331.1; NZ_UEFE01000037.1.
DR   AlphaFoldDB; P22708; -.
DR   SMR; P22708; -.
DR   PATRIC; fig|562.9739.peg.5216; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   CDD; cd01127; TrwB; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014128; T4SS_TraD.
DR   InterPro; IPR019476; T4SS_TraD_DNA-bd.
DR   InterPro; IPR022585; TraD_N.
DR   Pfam; PF12615; TraD_N; 1.
DR   Pfam; PF10412; TrwB_AAD_bind; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02759; TraD_Ftype; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell inner membrane; Cell membrane; Conjugation; Membrane;
KW   Nucleotide-binding; Plasmid; Repeat; Transmembrane; Transmembrane helix.
FT   CHAIN           1..738
FT                   /note="Coupling protein TraD"
FT                   /id="PRO_0000068449"
FT   TRANSMEM        28..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        113..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        396..413
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          617..619
FT                   /note="1"
FT   REPEAT          620..622
FT                   /note="2"
FT   REPEAT          623..625
FT                   /note="3"
FT   REPEAT          626..628
FT                   /note="4"
FT   REPEAT          629..631
FT                   /note="5"
FT   REPEAT          632..634
FT                   /note="6"
FT   REPEAT          635..637
FT                   /note="7"
FT   REPEAT          638..640
FT                   /note="8"
FT   REPEAT          641..643
FT                   /note="9"
FT   REPEAT          644..646
FT                   /note="10"
FT   REGION          607..690
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          617..646
FT                   /note="10 X 3 AA tandem repeats of [QE]-Q-P"
FT   COMPBIAS        624..641
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         192..199
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   738 AA;  83901 MW;  84CB1F48245E766F CRC64;
     MSFNAKDMTQ GGQIASMRIR MFSQIANIML YCLFIFFWIL IGLVLWVKIS WQTFINGCIY
     WWCTSLEGMR DLIKSQPVYE IQYYGKTFRM NAAQVLHDKY MIWCGEQLWS AFVLASVVAL
     VICLITFFVV SWILGRQGKQ QSENEVTGGR QLTDNPKDVA RMLKKDGKDS DIRIGDLPII
     RDSEIQNFCL HGTVGAGKSE VIRRLANYAR QRGDMVVIYD RSGEFVKSYY DPSIDKILNP
     LDARCAAWDL WKECLTQPDF DNTANTLIPM GTKEDPFWQG SGRTIFAEAA YLMRNDPNRS
     YSKLVDTLLS IKIEKLRTFL RNSPAANLVE EKIEKTAISI RAVLTNYVKA IRYLQGIEHN
     GDPFTIRDWM RGVREDQKNG WLFISSNADT HASLKPVISM WLSIAIRGLL AMGENRNRRV
     WFFCDELPTL HKLPDLVEIL PEARKFGGCY VFGIQSYAQL EDIYGEKAAA TLFDVMNTRA
     FFRSPSHKIA EFAAGEIGEK EHLKASEQYS YGADPVRDGV STGKDMERQT LVSYSDIQSL
     PDLTCYVTLP GPYPAVKLSL KYQARPKVAP EFIPRDINPE MENRLSAVLA AREAEGRQMA
     SLFEPEVASG EDVTQAEQPQ QPQQPQQPQQ PQQPQQPQQP QQPQQPVSSV INDKKSDAGV
     SVPAGGIEQE LKMKPEEEME QQLPPGISES GEVVDMAAYE AWQQENHPDI QQHMQRREEV
     NINVHRERGE DVEPGDDF