BUB2_YEAST
ID BUB2_YEAST Reviewed; 306 AA.
AC P26448; D6VZN0;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Mitotic check point protein BUB2;
DE AltName: Full=Cell cycle arrest protein BUB2;
GN Name=BUB2; OrderedLocusNames=YMR055C; ORFNames=YM9796.08C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1651171; DOI=10.1016/0092-8674(81)90014-3;
RA Hoyt M.A., Totis L., Roberts B.T.;
RT "S. cerevisiae genes required for cell cycle arrest in response to loss of
RT microtubule function.";
RL Cell 66:507-517(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP CHARACTERIZATION.
RX PubMed=11493673; DOI=10.1242/jcs.114.12.2345;
RA Lee S.E., Jensen S., Frenz L.M., Johnson A.L., Fesquet D., Johnston L.H.;
RT "The Bub2-dependent mitotic pathway in yeast acts every cell cycle and
RT regulates cytokinesis.";
RL J. Cell Sci. 114:2345-2354(2001).
CC -!- FUNCTION: Part of a checkpoint which monitors spindle integrity and
CC prevents premature exit from mitosis. This cell-cycle arrest depends
CC upon inhibition of the GTP-binding protein TEM1 by the BFA1/BUB2
CC complex.
CC -!- SUBUNIT: Interacts with BFA1.
CC -!- INTERACTION:
CC P26448; P47113: BFA1; NbExp=5; IntAct=EBI-3824, EBI-3586;
CC P26448; P38785: GIC1; NbExp=5; IntAct=EBI-3824, EBI-7575;
CC P26448; P38987: TEM1; NbExp=2; IntAct=EBI-3824, EBI-19113;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle pole.
CC -!- SIMILARITY: Belongs to the BUB2 family. {ECO:0000305}.
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DR EMBL; M64706; AAA16885.1; -; Genomic_DNA.
DR EMBL; Z49703; CAA89765.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09954.1; -; Genomic_DNA.
DR PIR; A39654; A39654.
DR RefSeq; NP_013771.1; NM_001182553.1.
DR AlphaFoldDB; P26448; -.
DR SMR; P26448; -.
DR BioGRID; 35231; 225.
DR ComplexPortal; CPX-964; GTPase-Activating Protein BFA1-BUB2 complex.
DR DIP; DIP-1215N; -.
DR IntAct; P26448; 44.
DR MINT; P26448; -.
DR STRING; 4932.YMR055C; -.
DR iPTMnet; P26448; -.
DR MaxQB; P26448; -.
DR PaxDb; P26448; -.
DR PRIDE; P26448; -.
DR TopDownProteomics; P26448; -.
DR EnsemblFungi; YMR055C_mRNA; YMR055C; YMR055C.
DR GeneID; 855077; -.
DR KEGG; sce:YMR055C; -.
DR SGD; S000004659; BUB2.
DR VEuPathDB; FungiDB:YMR055C; -.
DR eggNOG; KOG2058; Eukaryota.
DR GeneTree; ENSGT00730000111374; -.
DR HOGENOM; CLU_029367_2_0_1; -.
DR InParanoid; P26448; -.
DR OMA; RVNGWQS; -.
DR BioCyc; YEAST:G3O-32760-MON; -.
DR PRO; PR:P26448; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P26448; protein.
DR GO; GO:1990334; C:Bfa1-Bub2 complex; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0044732; C:mitotic spindle pole body; IBA:GO_Central.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0005816; C:spindle pole body; IDA:SGD.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0031578; P:mitotic spindle orientation checkpoint signaling; IDA:ComplexPortal.
DR GO; GO:1902543; P:negative regulation of protein localization to mitotic spindle pole body; IMP:SGD.
DR GO; GO:0031030; P:negative regulation of septation initiation signaling; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IDA:ComplexPortal.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Mitosis;
KW Reference proteome.
FT CHAIN 1..306
FT /note="Mitotic check point protein BUB2"
FT /id="PRO_0000208008"
FT DOMAIN 37..235
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
SQ SEQUENCE 306 AA; 35028 MW; A1DDBFB548E81EA3 CRC64;
MTSIEDLISN PPLLLHSSLS QLRYLILSEG LPISEDKQQQ RTRCYVWTVL SQTSMEASTQ
RYLALLKLGP PSTTIYQKIK NDTSRTFQTD PNFRNRVSED ALIRCLSCFA WQTQQRRQKT
RFGRIPVSTY VQGMNVLLAP LLYSCPSEPM AYQLFTKLCY EMIPTYLTKN LNGAQNGAKL
LDISLRIIDP KLSKFLSDNL LTAEIYGMPS ILTLSSCNKP LDQVIKLWDF MFAYGFHMNI
LFVVAFLVKM RSKVFKSDSP VNLLRQFPDF DADEIIRLGV GFIAKIPAQI YDLLVDHLTD
PDIYIP
