BUB31_ARATH
ID BUB31_ARATH Reviewed; 340 AA.
AC Q9LJN8;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 148.
DE RecName: Full=Mitotic checkpoint protein BUB3.1;
DE AltName: Full=Protein BUDDING UNINHIBITED BY BENZYMIDAZOL 3.1;
GN Name=BUB3.1; OrderedLocusNames=At3g19590; ORFNames=MMB12.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION, AND
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=18508582; DOI=10.2741/3076;
RA Lermontova I., Fuchs J., Schubert I.;
RT "The Arabidopsis checkpoint protein Bub3.1 is essential for gametophyte
RT development.";
RL Front. Biosci. 13:5202-5211(2008).
RN [6]
RP FUNCTION, INTERACTION WITH MAD2 AND BUBR1, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION.
RX PubMed=19710914; DOI=10.1371/journal.pone.0006757;
RA Caillaud M.-C., Paganelli L., Lecomte P., Deslandes L., Quentin M.,
RA Pecrix Y., Le Bris M., Marfaing N., Abad P., Favery B.;
RT "Spindle assembly checkpoint protein dynamics reveal conserved and
RT unsuspected roles in plant cell division.";
RL PLoS ONE 4:E6757-E6757(2009).
RN [7]
RP INTERACTION WITH CDC20-1 AND CDC20-2.
RX PubMed=21687678; DOI=10.1371/journal.pone.0020618;
RA Kevei Z., Baloban M., Da Ines O., Tiricz H., Kroll A., Regulski K.,
RA Mergaert P., Kondorosi E.;
RT "Conserved CDC20 cell cycle functions are carried out by two of the five
RT isoforms in Arabidopsis thaliana.";
RL PLoS ONE 6:E20618-E20618(2011).
CC -!- FUNCTION: Has a dual function in spindle-assembly checkpoint signaling
CC and in promoting the establishment of correct kinetochore-microtubule
CC (K-MT) attachments. Promotes the formation of stable end-on bipolar
CC attachments. Necessary for kinetochore localization of BUB1. The
CC BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting
CC complex or cyclosome (APC/C) when spindle-assembly checkpoint is
CC activated and inhibits the ubiquitin ligase activity of APC/C by
CC phosphorylating its activator CDC20 (By similarity). Essential for
CC gametophyte development. {ECO:0000250, ECO:0000269|PubMed:18508582,
CC ECO:0000269|PubMed:19710914}.
CC -!- SUBUNIT: Part of the mitotic checkpoint complex (MCC); interacts with
CC CDC20-1 and CDC20-2. Interacts with MAD2 and BUBR1.
CC {ECO:0000269|PubMed:19710914, ECO:0000269|PubMed:21687678}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore.
CC Cytoplasm, cytoskeleton, phragmoplast. Cytoplasm, cytoskeleton,
CC spindle. Note=Accumulates onto both kinetochores and the spindle
CC microtubules in cell arrested in metaphase. Starts to localize at
CC kinetochores in prometaphase I (Pro-MI) stage and maintains the
CC localization until the metaphase I-anaphase I (MI-AI) transition.
CC Associates with unattached kinetochores upon spindle assembly
CC checkpoint (SAC) activation. Present in the phragmoplast midline during
CC the final step of cell division.
CC -!- TISSUE SPECIFICITY: Expressed in actively dividing tissues, early in
CC organ development, in young leaves, lateral root primordia and root
CC meristems, flower buds, flowers and siliques.
CC {ECO:0000269|PubMed:18508582, ECO:0000269|PubMed:19710914}.
CC -!- INDUCTION: Cell cycle regulated expression with a distinct expression
CC peak at the G2/M boundary. {ECO:0000269|PubMed:18508582,
CC ECO:0000269|PubMed:19710914}.
CC -!- DISRUPTION PHENOTYPE: Survives only in heterozygous state. Displays
CC defects in development of male and female gametophytes.
CC {ECO:0000269|PubMed:18508582}.
CC -!- SIMILARITY: Belongs to the WD repeat BUB3 family. {ECO:0000305}.
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DR EMBL; AP000417; BAB02543.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76263.1; -; Genomic_DNA.
DR EMBL; BT004273; AAO42274.1; -; mRNA.
DR EMBL; BT006152; AAP04137.1; -; mRNA.
DR EMBL; AY087404; AAM64953.1; -; mRNA.
DR PIR; T52386; T52386.
DR RefSeq; NP_566644.1; NM_112849.5.
DR AlphaFoldDB; Q9LJN8; -.
DR SMR; Q9LJN8; -.
DR BioGRID; 6829; 17.
DR IntAct; Q9LJN8; 13.
DR STRING; 3702.AT3G19590.1; -.
DR PaxDb; Q9LJN8; -.
DR PRIDE; Q9LJN8; -.
DR ProteomicsDB; 240297; -.
DR EnsemblPlants; AT3G19590.1; AT3G19590.1; AT3G19590.
DR GeneID; 821496; -.
DR Gramene; AT3G19590.1; AT3G19590.1; AT3G19590.
DR KEGG; ath:AT3G19590; -.
DR Araport; AT3G19590; -.
DR TAIR; locus:2091211; AT3G19590.
DR eggNOG; KOG1036; Eukaryota.
DR HOGENOM; CLU_038526_0_0_1; -.
DR InParanoid; Q9LJN8; -.
DR OMA; WDPFNRK; -.
DR OrthoDB; 1048963at2759; -.
DR PhylomeDB; Q9LJN8; -.
DR PRO; PR:Q9LJN8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LJN8; baseline and differential.
DR Genevisible; Q9LJN8; AT.
DR GO; GO:1990298; C:bub1-bub3 complex; IBA:GO_Central.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:TAIR.
DR GO; GO:0000776; C:kinetochore; IDA:TAIR.
DR GO; GO:0005828; C:kinetochore microtubule; IDA:UniProtKB.
DR GO; GO:0033597; C:mitotic checkpoint complex; IBA:GO_Central.
DR GO; GO:0009524; C:phragmoplast; IDA:TAIR.
DR GO; GO:0005876; C:spindle microtubule; IDA:UniProtKB.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IDA:TAIR.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 3.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Cytoplasm; Cytoskeleton; Kinetochore; Meiosis; Mitosis; Nucleus;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..340
FT /note="Mitotic checkpoint protein BUB3.1"
FT /id="PRO_0000423380"
FT REPEAT 15..54
FT /note="WD 1"
FT REPEAT 96..135
FT /note="WD 2"
FT REPEAT 142..179
FT /note="WD 3"
FT REPEAT 239..278
FT /note="WD 4"
FT REPEAT 281..324
FT /note="WD 5"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 340 AA; 37874 MW; 73BA7A0385617733 CRC64;
MTTVTPSAGR ELSNPPSDGI SNLRFSNNSD HLLVSSWDKR VRLYDVSTNS LKGEFLHGGA
VLDCCFHDDF SGFSVGADYK VRRIVFNVGK EDILGTHDKA VRCVEYSYAA GQVITGSWDK
TVKCWDPRGA SGPERTQVGT YLQPERVYSM SLVGHRLVVA TAGRHVNIYD LRNMSQPEQR
RESSLKYQTR CVRCYPNGTG YALSSVEGRV AMEFFDLSEA AQAKKYAFKC HRKSEAGRDI
VYPVNSIAFH PIYGTFATGG CDGFVNIWDG NNKKRLYQYS KYPTSISALS FSRDGQLLAV
ASSYTFEEGE KSQEPEAIFV RSVNEIEVKP KPKVYPNPAA
